Fluorescence detection in capillary electrophoresis

Veleado, M. T.; Lara-Quintanar, Pilar; Frutos, Mercedes de; Diez-Masa, Jose Carlos

doi:10.1016/s0166-526x(05)45006-0

Cited by 9 publications

(12 citation statements)

References 235 publications

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“…We found in a previous study, in which several amino acids were on-column derivatized with FQ, that peak height increased with KCN concentration [17]. However, at high KCN concentrations, several broad peaks appeared in the electropherograms, probably due to reactions between impurities of FQ and KCN.…”

Section: Methods Developmentmentioning

confidence: 69%

“…Previous results in our laboratory showed that the derivatization reaction for amino acids is controlled by the pH of the buffer, mixing time, reaction time, reagent concentration, and reaction temperature [17]. In the present work, the effect of these parameters was explored for a mixture of BSA, b-LG, and a-LA at final concentrations 1610 -7 M for BSA and a-LA and 2610 -7 M for b-LG.…”

Section: Methods Developmentmentioning

confidence: 96%

“…The laboratory-made CE apparatus employed was described previously [17]. Briefly, high voltage was provided by a model RS/EH50R (Glassman High Voltage, Whitehouse Station, NJ) power supply.…”

Section: Instrumentationmentioning

confidence: 99%

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Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Veledo¹,

Frutos²,

Diez-Masa³

2005

J of Separation Science

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The main whey proteins have been derivatized on-capillary with 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ) and analyzed using a laboratory-made capillary electrophoresis apparatus provided with a laser-induced fluorescence detector. Several parameters controlling on-capillary derivatization of proteins, including pH, mixing time, reaction time, concentration of the reagents (potassium cyanide and FQ), and reaction temperature, were optimized. Coefficient variations were lower than 1% for migration time and 7% for peak height. Assay detection limits for the different proteins were in the range 5 nM to 10 nM. The method developed was applied to the separation of the major whey proteins in a laboratory-made cheese whey and in an infant food formulated with milk. In addition, the beta-LG content of these samples was quantitated. The results showed good agreement with those given by an RP-HPLC method and with those reported in the literature.

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Section: Methods Developmentmentioning

confidence: 69%

Section: Methods Developmentmentioning

confidence: 96%

See 1 more Smart Citation

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Veledo¹,

Frutos²,

Diez-Masa³

2005

J of Separation Science

Self Cite

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“…Ru(bpy)3 2+ * ⎯→ Ru(bpy)3 2+ + hv (6) It was found that with the aid of CPM (RR′NCH2R″), the quantity of Ru(bpy)3 2+ could be greatly enhanced while Ru(bpy)3 3+ decreased; therefore, the oxide current dramatically increased with a decrease in the reduce current.…”

Section: Cyclic Voltammograms Of Ru(bpy)3 2+ and Cpmmentioning

confidence: 99%

“…It has been widely used in the analysis of inorganic ions, organic substances, amino acids, DNA, protein, and so on. Various detection methods for CE are available, including UV-visible absorbance, 5 fluorescence, 6 mass spectrometry, 7 electrochemistry 8 and electrochemiluminescence (ECL). 9 Among them, ECL, as a special type of chemiluminescence (CL) in that the luminescence reagent is initiated by electrochemical reactions, has become an important and valuable detection technique in analytical chemistry in recent years.…”

mentioning

confidence: 99%

Determination of Chlorpheniramine and Its Binding with Human Serum Albumin by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

Liu

Zhou

2006

ANAL. SCI.

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Capillary Gel Electrophoresis

Kerékgyártó

Guttman

2015

Analytical Separation Science

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Capillary gel electrophoresis is a rapidly developing technique, which has been successfully used in the bioanalytical field, especially for separation of biopolymers like nucleic acids, proteins, and complex carbohydrates. This chapter starts with a summary of the theoretical and practical aspects of capillary gel electrophoresis including separation principles, instrument design, capillary coating techniques, and the most frequently used sieving matrices. It is followed by the description of the various detection options, such as UV ‐ Vis , diode‐array, laser‐induced fluorescence, and light‐emitting diode‐induced fluorescence types. Finally, an overview of the key application areas is given with the main emphasis on nucleic acid, protein, and complex carbohydrate analysis, as well as the latest developments in microchip electrophoresis devices.

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Fluorescence detection in capillary electrophoresis

Cited by 9 publications

References 235 publications

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Determination of Chlorpheniramine and Its Binding with Human Serum Albumin by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

Capillary Gel Electrophoresis

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