Fluorescence correlation spectroscopy of CdSe/ZnS quantum dot optical bioimaging probes with ultra-thin biocompatible coatings

Murcia, Michael J.; Shaw, David L.; Long, Eric C.; Naumann, Christoph

doi:10.1016/j.optcom.2007.07.069

Cited by 40 publications

(24 citation statements)

References 47 publications

(60 reference statements)

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“…The surfaces of naked Qdots are occupied by hydrophobic ligands from theorganometallic compounds during the syntheses of Qdots, such as the stabilizing ligand, trioctylphosphine oxide (TOPO), or hexadecylamine 92 . Ligand capping strategy exploits the physical interaction between hydrophobic ligand from Qdots and hydrophobic part of an amphiphilic polymer.…”

Section: Inorganic Dyes Enabled Biodegradable Fluorescent Polymersmentioning

confidence: 99%

Design strategies for fluorescent biodegradable polymeric biomaterials

2013

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The marriage of biodegradable polymer and fluorescent imaging has resulted in an important area of polymeric biomaterials: biodegradable fluorescent polymers. Researchers have put significant efforts on developing versatile fluorescent biomaterials due to their promising in biological/biomedical labeling, tracking, monitoring, imaging, and diagnostic applications, especially in drug delivery, tissue engineering, and cancer imaging applications. Biodegradable fluorescent polymers can function not only as implant biomaterials but also as imaging probes. Currently, there are two major classes of biodegradable polymers used as fluorescent materials. The first class is the combination of non-fluorescent biodegradable polymers and fluorescent agents such as organic dyes and quantum dots. Another class of polymers shows intrinsic photoluminescence as polymers by themselves carrying integral fluorescent chemical structures in or pendent to their polymer backbone, such as Green Fluorescent protein (GFP), and the recently developed biodegradable photoluminescent polymer (BPLP). Thus there is no need to conjugate or encapsulate additional fluorescent materials for the latter. In the present review, we will review the fluorescent biodegradable polymers with emphases on material fluorescence mechanism, design criteria for fluorescence, and their cutting-edge applications in biomedical engineering. We expect that this review will provide insightful discussion on the fluorescent biomaterial design and lead to innovations for the development of the next generation of fluorescent biomaterials and fluorescence-based biomedical technology.

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Section: Inorganic Dyes Enabled Biodegradable Fluorescent Polymersmentioning

confidence: 99%

Design strategies for fluorescent biodegradable polymeric biomaterials

2013

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“…The most popular are CdSe/ZnS QDs due to their bright and unique emission with the wide excitation spectra and narrow emission bandwidths (Bailey et al, 2004;Dybiec et al, 2007;Jamieson et al, 2007;Kune et al, 2001;Norris et al, 1996;Tessler et al, 2002). The II-VI QDs have been investigated in versatile photonic applications including solar cells (Choi et al, 2006;Kongkanand et al, 2008;Lopez-Luke et al, 2008), optical fibre amplifiers (Liu et al, 2007), color displays using light-emitting diode arrays (Huang et al, 2008: Klude et al, 2002Zhao et al, 2006), optical temperature probes (Liang et al, 2006;Walker et al, 2003), as well as in biology and medicine (Alivisatos et al, 2005;Grodzinski et al, 2006;Hoshino et al, 2007;Murcia et al, 2008;Portney & Ozkan, 2006;Wang et al, 2007). Note that metal, semiconductor, polymer and ceramic nanoparticles in general have gained essential interest for biological and medical applications (Brigger, et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Semiconductor II-VI Quantum Dots with Interface States and Their Biomedical Applications

Torchynska¹,

Vorobiev²

2011

Advanced Biomedical Engineering

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“…The thicker LIPO coating was chosen because it is very stable and was already successfully applied in long-term cellular imaging studies 38. The thinner AEE coating was pursued because the overall probe size can be reduced and because a similar coating, which is based on short poly(ethylene oxide) chains of just two ethylene oxide segments, was shown to prevent the non-specific adsorption of proteins quite effectively 39. As reported recently, confocal fluorescence correlation spectroscopy (FCS) is employed to verify the colloidal stability of AEE - and LIPO -coated QDs and to determine their hydrodynamic radii 40.…”

Section: Introductionmentioning

confidence: 99%

Design of Quantum Dot-Conjugated Lipids for Long-Term, High-Speed Tracking Experiments on Cell Surfaces

et al. 2008

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The current study reports the facile design of quantum dot (QD)-conjugated lipids and their application to high-speed tracking experiments on cell surfaces. CdSe/ZnS core/shell QDs with two types of hydrophilic coatings, 2-(2-aminoethoxy)ethanol (AEE-coating) and a 60:40 molar mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethyleneglycol-2000] (LIPO-coating), are conjugated to sulfhydryl lipids via maleimide reactive groups on the QD surface. Prior to lipid conjugation, the colloidal stability of both types of coated QDs in aqueous solution is confirmed using fluorescence correlation spectroscopy. A sensitive assay based on single lipid tracking experiments on a planar solid-supported phospholipid bilayer is presented that establishes conditions of monovalent conjugation of QDs to lipids. The QD lipids are then employed as single molecule tracking probes in plasma membranes of several cell types. Initial tracking experiments at a frame rate of 30 fps corroborate that QD-lipids diffuse like dye-labeled lipids in the plasma membrane of COS-7, HEK-293, 3T3, and NRK cells, thus confirming monovalent labeling. Finally, QD-lipids are applied for the first time to high-speed single molecule imaging by tracking their lateral mobility in the plasma membrane of NRK fibroblasts with up to 1000 fps. Our high-speed tracking data, which are in excellent agreement to previous tracking experiments with larger 40nm Au labels, not only push the time resolution in long-time, continuous fluorescence-based single molecule tracking, but also show that highly photostable, photoluminescent nanoprobes of 10nm size can be employed (AEE-coated QDs). These probes are also attractive because, unlike Au nanoparticles, they facilitate complex multicolor experiments.

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Fluorescence correlation spectroscopy of CdSe/ZnS quantum dot optical bioimaging probes with ultra-thin biocompatible coatings

Cited by 40 publications

References 47 publications

Design strategies for fluorescent biodegradable polymeric biomaterials

Design strategies for fluorescent biodegradable polymeric biomaterials

Semiconductor II-VI Quantum Dots with Interface States and Their Biomedical Applications

Design of Quantum Dot-Conjugated Lipids for Long-Term, High-Speed Tracking Experiments on Cell Surfaces

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