Fluorescence Characteristics of 5-Hydroxytryptamine (Serotonin)

Udenfriend, Sidney; Bogdanski, Donald F.; Weissbach, Herbert

doi:10.1126/science.122.3177.972

Cited by 140 publications

(52 citation statements)

References 2 publications

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“…Webb and co-workers have shown that the emission arises from an overall 6-photon process with 830 nm photons, and may be resolved into a 4-photon excitation of 5HT to the transient fluorescing intermediate, and a 2-photon excitation of fluorescence 4 . Luminescence in the region of 550 nm has also been reported from 5-hydroxytryptamine in acidic solutions 8 . Shear et al 4 suggest that multiphoton-induced hyperluminescence from 5HT might arise from the 5-indoxyl radical, known to be formed by photoionisation of 5HT by 248 nm laser flash photolysis 9 , and which has also been studied by pulse radiolysis [10][11][12][13] .…”

Section: Introductionmentioning

confidence: 79%

Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan

Bisby

Arvanitidis

Botchway

et al. 2003

Photochem Photobiol Sci

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It is reported (Shear et al, Photochem.Photobiol. 65, 931 (1997) ) that multiphoton near infrared excitation of 5-hydroxytryptophan results in a transient product with green fluorescence. Visible fluorescence from multiphoton excitation enables detection of 5-hydroxytryptophan with extremely high sensitivity and also has potential applications in imaging of biological systems and investigation of protein dynamics. The characteristic fluorescence at 500 nm has now also been observed on one-photon photolysis of solutions at 308 nm, followed by excitation of fluorescence at 430 nm. Fluorescence was observed in aerated and deaerated solutions and in the presence of ascorbate. Enhancement of fluorescence was observed on addition of ethanol. Transient absorption experiments with 308 nm photolysis showed the formation of three transient species. In the presence of ascorbate the radical formed by photoionisation was removed, revealing a long-lived species (  > 1 ms) with a similar absorption spectrum, which is ascribed to the fluorescing species. Fluorescence induced by multiphoton excitation had a lifetime of 910 ± 10 ps and was also unaffected by ascorbate. In the presence of organic solvents there was an increase in fluorescence lifetime, but a decrease in overall fluorescence intensity. The fluorescence intensity and fluorescence lifetime both decreased in acidic solution (pH < 3). It is suggested that the fluorescence originates not from the 5-indoxyl radical, but from a transient product formed by intramolecular rearrangement.2

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Section: Introductionmentioning

confidence: 79%

Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan

Bisby

Arvanitidis

Botchway

et al. 2003

Photochem Photobiol Sci

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“…It is unlikely that brain 5-HT is derived from circulating blood 5-HT, as 5-HT will not penetrate the blood-brain barrier (a fact confirmed by the absence of mental symptoms in patients with argentaffinoma). On the other hand 5-hydroxytryptophan penetrates the blood-brain barrier readily (Udenfriend, Bogdanski and Weissbach, 1956) and injection of 5-hydroxytryptophan, unlike 5-HT, will cause a rise in brain 5-HT levels, as is to be expected from the presence of 5-hydroxytryptophan decarboxylase in the brain.…”

mentioning

confidence: 99%

“…The following modifications were made: (a) to obtain good recoveries of 5-HT it was found essential to have the pH of the borate buffer between 9-5 and 9*8; and (b) the 5-HT was finally extracted into 3N-HC1 instead of dilute HC1, and the 5-HT determined fluorimetrically (cf. Udenfriend, Bogdanski and Weissbach, 1955) using the fluorimeter of Laurence (1957), instead of colorimetrically. The filter for the exciting light was a 3 cm.…”

mentioning

confidence: 99%

Biogenesis of 5-Hydroxytryptophan

Dalgliesh¹,

Dutton²

1957

Br J Cancer

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“…5-Hydroxytryptamine was extracted with n-butanol by the method of Udenfriend, Weissbach, and Clark (1955), as modified by Cargill-Thompson, Jiardwick, and Wiseman (1958) and Baker (1959), and assayed on the rat gastric fundus preparation (Vane, 1957).…”

Section: Methods Of Analysismentioning

confidence: 99%

The Distribution of 5‐hydroxytryptamine and Adenosinetriphosphate in Cytoplasmic Particles of the Dog's Small Intestine

Prusoff

1960

British Journal of Pharmacology and Chemotherapy

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The distribution of 5‐hydroxytryptamine, adenosinetriphosphate, and succinic dehydrogenase in sucrose homogenates of the dog's small intestine has been studied. The adenosinetriphosphate was present in two different layers which could be separated by density gradient centrifugation. The upper layer contained also much succinic dehydrogenase, but no amine; it is probably composed of mitochondria. The lower layer contained not only adenosinetriphosphate but also the major portion of the particle‐held 5‐hydroxytryptamine. The mean molar ratio, amine: adenosinetriphosphate, in the lower layer was 2.6. The experiments suggest that adenosinetriphosphate in the intestine is of importance in the storage of 5‐hydroxytryptamine, resembling the function of adenosinetriphosphate in the storage of the catechol amines of the adrenal medulla.

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Fluorescence Characteristics of 5-Hydroxytryptamine (Serotonin)

Cited by 140 publications

References 2 publications

Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan

Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan

Biogenesis of 5-Hydroxytryptophan

The Distribution of 5‐hydroxytryptamine and Adenosinetriphosphate in Cytoplasmic Particles of the Dog's Small Intestine

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