Fluorescence-based immunochromatographic test strip for the detection of hyoscyamine

Abstract

Hyoscyamine (HSM) which act as antagonists of acetylcholine muscarinic receptor and can induce a variety of distinct toxic syndromes in mammals (anti-cholinergic poisoning) which hazardous in human health. Therefore, it...

Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

“…Gold nanoparticles (GNPs) were obtained and conjugated with the mAb as described in our previous reports. 34,37…”

“…Gold nanoparticles (GNPs) were obtained and conjugated with the mAb as described in our previous reports. 34,37 Fig. 3 The LFICS consists of a sample pad, nitrocellulose (NC) membrane, an absorbent pad, and a plastic backing sheet.…”

Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange

“…We conducted ic-ELISA as previously reported. 30,33 Briefly, 96-well microplates were coated with 100 μL of coatingantigen in CBS (15 mM Na 2 CO 3 and 34.9 mM NaHCO 3 , pH 9.6), incubated at 37 °C for 2 h, and blocked with 200 μL of blocking buffer (0.2% gelatin in CBS) for 2 h. After washing, we added 50 μL of flumetralin standard and 50 μL of mAb to each well and incubated the microplates at 37 °C for 30 min. Following another wash, we added 100 μL of HRPlabeled goat anti-mouse IgG and incubated the plates for 30 min.…”

“…Gold nanoparticles (GNPs) of ∼15 nm in diameter were synthesized. 33,34 K 2 CO 3 solution (0.1 M) was added to the GNP solution to achieve a pH value of 8.8. MAb 3F5 was added dropwise to the solution and incubated for 1 h at room temperature.…”

Detection of Highly Poisonous Nerium oleander Using Quantitative Real-Time PCR with Specific Primers

“…The absence of diagnostic characteristics restricts the application of morphological and microscopic identification. Chemical analyses based on thin-layer chromatography, high-performance liquid chromatography, and gas chromatography are presently the most popular methods for toxic component testing [21,23]. Nevertheless, for samples with complex and varied compositions, tedious cleanup procedures and precolumn derivatization are usually required to remove interference compounds and improve detection sensitivity, which results in a time-consuming process [24].…”