Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

Xu, Xinxin; Guo, Lingling; Kuang, Hua; Xu, Liguang; Liu, Liqiang

doi:10.1039/d2an01366e

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…Compared with the reported anti-CAP mAb (IC 50 , 1.6 ng/mL [18] and 1.5 ng/mL [19]), the sensitivity was improved 6.7-fold and 6.3-fold. Only cyclaniliprole and tetraniliprole showed CRs of 28.8% and 0.3%, respectively, because they have an antraniloiyl group (Table S2), and were similar to those reported previously (CR for cyclaniliprole was 37.5% [19,32].…”

Section: Characterization Of Anti-cap Mabsupporting

confidence: 87%

“…Therefore, the CRs were 5% for tetraniliprole and 50% for cyclaniliprole using the AuNP-LFIA, which were similar to the The comparison between the AuNP-LFIA developed in this study and other methods for CAP is shown in Table S3. The sensitivity of the AuNP-LFIA in this study (visible LOD was 1.25 ng/mL) was improved 2-fold compared to the LFIA reported previously (visible LOD was 2.5 ng/mL) [32]. Although the proposed AuNP-LFIA had similar sensitivity to ELISAs [18,19], the detection time was reduced by more than 13-fold.…”

Section: Specificity Of the Aunp-lfiamentioning

confidence: 43%

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A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

Wu,

Li,

Zhu

et al. 2024

Foods

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Chlorantraniliprole (CAP) is a new type of diamide insecticide that is mainly used to control lepidopteran pests. However, it has been proven to be hazardous to nontarget organisms, and the effects of its residues need to be monitored. In this study, five hybridoma cell lines were developed that produced anti-CAP monoclonal antibodies (mAbs), of which the mAb originating from the cell line 5C5B9 showed the highest sensitivity and was used to develop a gold nanoparticle-based lateral flow immunoassay (AuNP-LFIA) for CAP. The visible limit of detection of the AuNP-LFIA was 1.25 ng/mL, and the detection results were obtained in less than 10 min. The AuNP-LFIA showed no cross-reactivity for CAP analogs, except for tetraniliprole (50%) and cyclaniliprole (5%). In the detection of spiked and blind samples, the accuracy and reliability of the AuNP-LFIA were confirmed by a comparison with spiked concentrations and verified by ultra-performance liquid chromatography–tandem mass spectrometry. Thus, this study provides the core reagents for establishing CAP immunoassays and a AuNP-LFIA for the detection of residual CAP.

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Section: Characterization Of Anti-cap Mabsupporting

confidence: 87%

Section: Specificity Of the Aunp-lfiamentioning

confidence: 43%

A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

Wu,

Li,

Zhu

et al. 2024

Foods

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show abstract

“…Immunogen CBD hapten BSA (CBD-HS-BSA) and coating antigen CBD hapten ovalbumin (CBD-HS-OVA) were produced by coupling CBD-HS with BSA and OVA via an active ester method [35]. Activation solution was prepared by dissolving CBD (2.7 mg), N-hydroxysuccinimide (NHS, 1.5 mg), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 2.5 mg) in N, N-dimethylformamide (DMF, 0.5 mL) and stirring for 6 h at 25 • C. Then, the mixture was added slowly into BSA solution (20.0 mg of BSA added into 0.01 M PBS, 2 mL), stirred for 8 h, and subsequently dialyzed in PBS (pH 7.4) for 72 h at 4 • C to isolate CBD-HS-BSA.…”

Section: Preparation Of Antigenmentioning

confidence: 99%

High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor

Sun,

Zhu,

Tao

et al. 2023

Biosensors

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In order to facilitate monitoring of cannabidiol (CBD), we devised a gold immunochromatographic sensor based on a specific monoclonal antibody (mAb). To prepare the antigen, a novel hapten with CBD moiety and a linear carbon chain was employed. By utilizing hybridoma technology, a specific mAb was screened and identified that exhibited a 50% maximal inhibitory concentration against CBD ranging from 28.97 to 443.97 ng/mL. Extensive optimization led to the establishment of visual limits of detection for CBD, achieving a remarkable sensitivity of 8 μg/mL in the assay buffer. To showcase the accuracy and stability, an analysis of CBD-spiked wine, sparkling water, and sports drink was conducted. The recovery rates observed were as follows: 88.4–109.2% for wine, 89.9–107.8% for sparkling water, and 83.2–95.5% for sports drink. Furthermore, the coefficient of variation remained impressively low, less than 4.38% for wine, less than 2.07% for sparkling water, and less than 6.34% for sports drink. Importantly, the developed sensor exhibited no cross-reaction with tetrahydrocannabinol (THC). In conclusion, the proposed paper sensor, employing gold nanoparticles, offers a user-friendly and efficient approach for the precise, rapid, and dependable determination of CBD in products.

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Rapid and simultaneous detection of five mycotoxins and their analogs with a gold nanoparticle-based multiplex immuno-strip sensor

Zhou,

Zhu,

Song

et al. 2024

Food Microbiology

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Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

Abstract: Anthranilic diamide insecticides, including chlorantraniliprole (CHL), cyantraniliprole (CYA), cyclaniliprole (CYC), and tetrachlorantraniliprole (TEA), are widely used in agricultural production, resulting in potential risk to human health. In this study, we...

Cited by 5 publications

References 38 publications

A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor

Rapid and simultaneous detection of five mycotoxins and their analogs with a gold nanoparticle-based multiplex immuno-strip sensor

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