Fluorescence-based assay with enzyme amplification on a micro-flow immunosensor chip for monitoring coplanar polychlorinated biphenyls

Localized surface plasmon resonance based optical biosensor using surface modified nanoparticle layer for label-free monitoring of antigen–antibody reaction

“…In the past decade, various kinds of biosensors were developed, and biosensor became a part of many fields in research, such as medicine [1], and environmental monitoring [2]. Biosensor experiments involve immobilizing a ligand on a solid substrate surface, and monitoring its interaction with an analyte in sample solution.…”

Dimensionless model for impedimetric sensing of particle laden droplets in digital microfluidic devices

“…As a result, these miniature devices can be used to create portable medical diagnostic units that can be deployed at the point of care (Lee and Lee 2004;Endo et al 2005;Sista et al 2008b;Shah and Kim 2009;Miller et al 2011;Ng et al 2012Ng et al , 2013Hung et al 2015). Lowcost, portable LOC devices provide an opportunity for large-scale public health screening of various medical conditions (Jain et al 2015).…”

“…The model also predicts that particle concentration and measurement frequency can be used to independently control sensitivity Many microfluidic LOC devices perform chemical reactions by binding proteins or other biological material to particles suspended in the flow (Endo et al 2005;Sista et al 2008b;Shah and Kim 2009;Ng et al 2012). These particles can be suspended in a continuous flow in channel based microfluidics (Endo et al 2005), or in a droplet in discrete flow microfluidics (Sista et al 2008b;Shah and Kim 2009;Ng et al 2012). As such, design parameters including: particle size, particle materials, and device geometry should be optimized to maximize detection sensitivity in each particular application.…”

“…Optical detection of a fluorescent or colorimetric signal may be the most common method for process monitoring in microfluidic devices (Endo et al 2005;Sista et al 2008a;Miller et al 2011;Ng et al 2012). This signal is generally detected using external or imbedded fluorescence imaging, electrochemiluminescence, or chemoreceptive neuron MOS transistors (Shen et al 2004;Endo et al 2005;Satoh et al 2005;Luan et al 2008;Miller and Wheeler 2008;Miller et al 2011). Real-time capacitive monitoring of droplet composition and mixing of inert droplets in DMFs has been validated analytically and experimentally (Schertzer et al 2010a).…”

Label-free bioelectronic immunoassay for the detection of human telomerase reverse transcriptase in urine

“…In the past decade, highly advanced transducers enabled the label-free detection of the interaction between proteins, that is, when none of the proteins is modified with a label; such as fluorescent dyes [25,26], has become possible with optical, including surface plasmon resonance (SPR) [27] and localized SPR [28,29] piezoelectrical, such as quartz crystal microbalance (QCM) [30]. Recently, electrochemical detection of proteins has become an important topic in bioanalysis [31][32][33][34].…”