Label-free bioelectronic immunoassay for the detection of human telomerase reverse transcriptase in urine

Takata, Masayuki; Kerman, Kağan; Nagatani, Naoki; Konaka, Hiroyuki; Namiki, Mikio; Tamiya, Eiichi

doi:10.1016/j.jelechem.2006.07.025

Cited by 15 publications

(9 citation statements)

References 43 publications

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“…Oxidation signal of the monoclonal antibody against T-PSA (T-PSA-MAb) significantly increased on the interaction of T-PSA with the SWCNTimmobilized TPSA-MAb. The same group developed an immunoassay for human telomerase reverse transcriptase (hTERT) based on the protein electroactivity at carbon electrodes [162]. Instead of SWCNT-modified carbon electrode they used pencil graphite electrode, to which the hTERT monoclonal antibody (hTERT-MAb) was immobilized via protein A. Interaction of this electrode with cell lysate from urine of bladder cancer patients resulted in a decrease of the hTERT-MAb oxidation peak, i.e.…”

Section: Oxidation Of Dna And/or Proteins At Carbon Electrodesmentioning

confidence: 99%

Electroactivity of Nonconjugated Proteins and Peptides. Towards Electroanalysis of All Proteins

2007

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Present proteomics and biomedicine require sensitive analytical methods for all proteins. Recent progress in electrochemical analysis of peptides and proteins based on their intrinsic electroactivity is reviewed. Tyrosine and/or tryptophan-containing proteins are oxidizable at carbon electrodes. At mercury electrodes all peptides and proteins (about 13 peptides and > 25 proteins were tested) produce chronopotentiometric peak H at nanomolar concentrations. This peak is sensitive to changes in protein structure. Microliter sample volumes are sufficient for the analysis. Electrochemical methods can be used in studies of nucleic acid-protein interactions and can be applied in biomedicine. Examples of such applications in neurogenerative diseases and cancer are presented.

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Section: Oxidation Of Dna And/or Proteins At Carbon Electrodesmentioning

confidence: 99%

Electroactivity of Nonconjugated Proteins and Peptides. Towards Electroanalysis of All Proteins

2007

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“…In this proof‐of‐concept study, the electrochemical techniques have been employed to detect the interaction of ApoE4, ApoE3 and ApoE2 with two isoforms of Aβ (Aβ 1–40 and Aβ 1–42 ) on gold screen printed electrodes (AuSPEs) using streptavidin (SA) as a linker between the electrode surface and Aβ. The electrochemical biosensor procedure is cost‐effective, rapid and only requires a low volume (5–10 μL) of reagents.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Isoform‐Specific Interaction between Apolipoprotein E and Amyloid‐β

Jin

Noroozifar

et al. 2018

ChemElectroChem

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Alzheimer's disease (AD) is characterized by the formation of neurofibrillary tangles (NFTs) including amyloid-β (Aβ) in the brain. Apolipoprotein E (ApoE) plays a key role in the homeostasis of cholesterol and other lipids in blood circulation. The interaction between ApoE and Aβ has been implicated in the pathological progression of AD. In this study, the interaction of three isoforms of ApoE4, ApoE3 and ApoE2 with Aβ 1-40 and Aβ 1-42 peptides was investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Interactions between Aβ peptides and ApoE isoforms were determined by monitoring the changes in ΔR ct of Nyquist plots. The affinity between Aβ 1-40 and Aβ 1-42 and ApoE isoforms interpreted from EIS data followed the trend: ApoE4 > ApoE3 > ApoE2. Using surface plasmon resonance (SPR), the binding affinity (K D ) constants for the interaction of ApoE4 with Aβ 1-40 and Aβ 1-42 peptides were determined as 185 � 21 nM and 156 � 18 nM, respectively, in agreement with our electrochemical results. Our proof-of-principle study demonstrates that EIS provides a promising platform for the investigation of protein-protein interactions implicated in AD.

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“…Human serum albumin (HSA) was analyzed by potentiometric stripping analysis through adsorbed anti-HSA IgG onto thick-film carbon working electrode [33]. Our group has reported the label-free detection of human telomerase reverse transcriptase (hTERT) for cancer diagnosis by adsorptive transfer differential pulse anodic stripping voltammetry using cell lysates prepared from urine samples from adults [135]. We have also fabricated a label-free electrochemical sensor (Fig.…”

Section: Diagnostic Applicationmentioning

confidence: 99%

Electrochemical Biosensors for Medical and Food Applications

2008

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Sensing and quantification of DNA and proteins are becoming increasingly important in biochemistry, medicine and biotechnology. Traditional analytical techniques are lagging behind the demand for more information in less time, at a lower cost. An important step forward in this pursuit is to identify an analyte using its electrochemical behavior and to convert its presence and concentration into perceivable and distinct electrical signals. This review covers the strategies for electrochemical sensing of biomolecules, mainly, DNA and proteins by label-based and label-free approaches using disposable electrochemical printed chips and carbon nanotube based field effect transistors. Issues, such as ease of preparation, robustness, sensitivity, and realization of mass production of the detection strategies are also considered. A good coverage of the published literature, mostly, a wide treatment of original research articles reporting novel principles has been made. Finally, this review may help the researchers in developing an understanding of miniaturized electrochemical biosensors and their possible applications in medical and food science, with directions for future research.

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Label-free bioelectronic immunoassay for the detection of human telomerase reverse transcriptase in urine

Cited by 15 publications

References 43 publications

Electroactivity of Nonconjugated Proteins and Peptides. Towards Electroanalysis of All Proteins

Electroactivity of Nonconjugated Proteins and Peptides. Towards Electroanalysis of All Proteins

Electrochemical Detection of Isoform‐Specific Interaction between Apolipoprotein E and Amyloid‐β

Electrochemical Biosensors for Medical and Food Applications

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