Fluorescence Analysis of Oat phyA Deletion Mutants Expressed in Tobacco Suggests that the N-Terminal Domain Determines the Photochemical and Spectroscopic Distinctions between phyA′ and phyA′′

Protochlorophyllide reduction - what is new in 2005?

“…1 and 2 are of no help for the understanding. The comparison of the FR effects on the change in the R value in tobacco transgenic lines over-expressing full-length PhyA or N-terminally truncated (∆7-69 oat PhyA), which belongs to the PhyA" (Sineshchekov et al 1999), leads to the conclusion that PhyA' is responsible for the mediation ). The effect would be inhibited by PhyA".…”

Two modes of the light-induced phytochrome A decline – with and without changes in the proportion of its isoforms (phyA′ and phyA″): evidence from fluorescence investigations of mutant phyA-3D pea

“…The experimental approach was the same as that used earlier (see [11][12][13][14]17,18,21,22] and the literature cited therein). To obtain the phytochrome parameters in the sample, low-temperature (85 K) emission spectra (wavelength of the excitation, k e , 610 or 650 nm) were taken under two different experimental settings (see Figs.…”

“…PhyA 0 is a major, variable by its concentration in plant tissues and light-labile species whereas phyA 00 is a minor, relatively constant by the level of expression in different tissues and less light-labile. The two phyA species are likely to differ by the character of post-translational modification, possibly, phosphorylation, within the 7-69 amino acid residue stretch of the N-terminus [14,15]. Physical interaction of phyA with PKS1 and PKS2 (phytochrome kinase substrates) may also have relevance to the formation of its isoforms [16].…”