Fluorescein mercury(II) acetate and sodium fluorescein as reagents for the determination of bis(2-chloroethyl)sulfide by fluorescence quenching

Hunt, Anya L.; Alder, John F.

doi:10.1016/s0003-2670(99)00110-5

Cited by 10 publications

(4 citation statements)

References 11 publications

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“…At this stage, fluorescence was induced with 10s flash/5min dark time cycle. Since 2-CEES is a reported fluorescence quencher [38], therefore for subsequent fluorimetric studies, higher concentration of chlorophyll i.e. 6mg/L was selected and illuminated with different combinations of flash and dark times.…”

Section: B Optimization Of Conditions For Fluorescence Studiesmentioning

confidence: 99%

Chlorophyll based biosensor for sulfur mustard - a chemical warfare agent

Kaur¹,

Singh²,

Verma³

2012

2012 1st International Symposium on Physics and Technology of Sensors (ISPTS-1)

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Sulfur mustard (SM), a chemical warfare agent (CWA) is a bifunctional blistering and alkylating agent used in military warfare having antimitotic, mutagenic, carcinogenic, teratogenic and cytotoxic effects. Conventional techniques used for the detection of CWAs are complex, expensive and require sophisticated analytical procedures thus entailing the development of alternative analytical tools. Biosensors offer an alternative analytical approach with a promise of selectivity in addition to sensitivity, ease of use, rapid response and negligible sample pre-treatment. Furthermore, biomolecules have the ability to detect toxicity in addition to concentration. This work reports the development of a fluorescence based biosensor for detection of SM. 2-chloroethyl ethyl sulfide (2-CEES), a sulfur mustard mimic which is structurally similar to it but not as lethal was used for the study, utilizing the ability of chlorophyll to detect the said compound owing to fluorescence. For this, chlorophyll extract from a plant source was immobilized on fibre glass discs of 5mm diameter, and its fluorescence was studied by excitation at 437 nm and emission at 667 nm. The exposure of the biocomponent to 2-CEES led to quenching of fluorescence, which varied linearly with increasing concentration of 2-CEES with a detection limit of 7.68 × 10 -10 M. The fluorescence drop mechanism was characterized by HPLC studies which confirmed the conversion of chlorophyll, upon exposure to the analyte, to non-fluorescing catabolic products. The low detection limit was a promising feature of the biosensor.

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Section: B Optimization Of Conditions For Fluorescence Studiesmentioning

confidence: 99%

Chlorophyll based biosensor for sulfur mustard - a chemical warfare agent

Kaur¹,

Singh²,

Verma³

2012

2012 1st International Symposium on Physics and Technology of Sensors (ISPTS-1)

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“…This uorescent compound has been used to quantitate various compounds by measuring the decrease in its uorescence intensity upon its reaction with a sulfur containing moiety. [41][42][43] This reaction was formerly applied for the estimation of penicillamine, 44 and for the quantitation of various anions such as sulde, [45][46][47] cyanide and iodide ions. 48 In the present work, AMF reacts with each of MSN, ACT and TMN in the presence of borate buffer, with subsequent measurement of the decrease in its absorbance at 497 nm in the spectrophotometric procedure (Method I), and measurement of the uorescence quenching of the reagent at l em ¼ 520 nm for the uorimetric procedure (Method II).…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric and spectrofluorimetric determination of mesna, acetylcysteine and timonacic acid through the reaction with acetoxymercuri fluorescein

et al. 2016

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“…Mercury-complexing agents, such as alkaline uorescein mercuric acetate (FMA), have mainly been employed for sulydryl determination. 2,26 Zinc, 27 ruthenium 28 and copper 29 have also been used as luminescent metal-based probes.…”

Section: Introductionmentioning

confidence: 99%