Fluidized Bed Design Parameters Affecting Novel Lactic Acid Downstream Processing

Abstract

Lactic acid purification was directly done from fermentation utilizing a fluidized bed column refilled with a strong anionic exchange resin. The purpose of this work was to study the influence of two important design parameters, bed-diameter (D) and bed-height (H), in the lactic acid binding and elution capacity of the matrix. By changing the settled bed height from 2.5 to 5 cm for each diameter of column analyzed it was possible to obtain an 50% increase in the binding capacity of the resin in all experiments. This fact was attributed to a higher contact time between the culture broth and the anionic resin produced by the increase of back mixing and lactic acid residence time.

Expanded bed adsorption of an alkaline lipase from Pseudomona cepacia

“…It introduces the concept that there is a stronger resistance of resin bed in order water (H 2 O) > phosphate buffer (PhB) > biomoleule + buffer (BB). For the fluidizer containing maize malt and salts, there was a large effect of friction on degree of expansion for the particle-particle, particle-liquid and particle-biomolecule interactions, which provoked an increase in the linear velocity of the fluidizer to maintain the bed voidage at the same level as that in the salt system [17,18,[24][25][26][27][28][29][30][31]. Analyzing these figures, it can be observed that the values of Richardson-Zaki index decreased in relation to temperature and to the addition of salt and of biomolecules.…”

“…It can be observed that the errors between the velocities are lower and they are associated with experimental measurement viscosity and specific mass and the reliability of the data in the table. The low error suggests that the Richardson-Zaki equation is the best empirical model to predict the hydrodynamic behavior of adsorbent particles in expanded bed systems, even when the fluidizer contains a larger amount of biological material in suspension [18,19,23,25,26,[28][29][30][31]. The fluidized particle end velocity decreased when the salt and biological materials were added.…”

“…It permits crude feeding into the chromatographic column without an initial treatment to eliminate the biological material suspension, and as the bed expands it increases adsorbent surface contact, making interaction with the targeted molecule more effective [16][17][18][19][20][21][22]. Presently, several studies on expanded bed adsorption behaviors allow as to better understand the effects of adsorbent type and size [17,18,[23][24][25][26][27], bed height, and linear velocity [17,18,[26][27][28][29][30][31] for application in the recovery of important biomolecules.…”

Role of Clay Substrate Molecular Interactions in Some Dairy Technology Applications

“…Here, excessive lactic bacteria biomass upon advanced fermentation level was also found to paradoxically produce less LA [122]. The inhibitory capacity of LA was also demonstrated by the detrimental effect of its prolonged contact time with lactic bacteria in fluidized bed-based extractive fermentation in the presence of anion exchangers [123]. Extractive fermentation involving in situ ion exchange is an interesting technique that allows for overcoming the detrimental effect of LA.…”

Energy and techno-economic analysis of bio-based carboxylic acid recovery by adsorption

“…21 That process binds the carboxylate anion to the resin and the elution of the bound carboxylate anion requires a concentrated acid solution such as 4 M hydrochloric acid. 51 Therefore, every mole of butyric acid requires one mole of sodium hydroxide to convert the butyric acid into carboxylate and one mole of hydrochloric acid (HCl) to elute. This process adds substantial raw material OPEX.…”