Fluidity of Chicken Ventricular Plasma Membranes During Development in-ovo and after Birth: Spin Labelling and Fluorescence Studies

Daveloose, Denis; Vezin, Hervé; Bassé, François; Viret, Jacques

doi:10.1006/jmcc.1993.1160

Cited by 5 publications

(4 citation statements)

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“…Fish were killed by a blow to the head and the liver quickly excised. Semi‐purified membranes were prepared using a modified method developed for chick hearts [22]. The liver was rinsed in Hanks' buffered saline (136.7 m m NaCl, 5.4 m m KCl, 0.8 m m MgSO 4 , 0.33 m m NaH 2 PO 4 , 0.44 m m KH 2 PO 4 , 5.0 m m Hepes, 5.0 m m Hepes‐Na, and 1.0 m m NaHCO 3 , pH 7.63), resuspended in 8 vol.…”

Section: Methodsmentioning

confidence: 99%

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A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

Nickerson

Dugan

Drouin

et al. 2001

European Journal of Biochemistry

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Extensive molecular characterization of mammalian b-adrenoceptors has revealed complex modes of regulation and interaction. Relatively little attention, however, has focused on adrenoceptors from early branching vertebrates such as fish. Using an RT-PCR approach we have cloned a rainbow trout b 2 -adrenoceptor gene that codes for a 409-amino-acid protein with the same seven transmembrane domain structure as its mammalian counterparts. This rainbow trout b 2 -adrenoceptor shares a high degree of amino-acid sequence conservation with other vertebrate b 2 -adrenoceptors. The conclusion that this sequence is a rainbow trout b 2 -adrenoceptor is further supported by phylogenetic analysis of vertebrate b-adrenoceptor sequences and competitive pharmacological binding data. RNase protection assays demonstrate that the rainbow trout b 2 -adrenoceptor gene is highly expressed in the liver and red and white muscle, with lower levels of expression in the gills, heart, kidney and spleen of the rainbow trout. The lack of regulatory phosphorylation sites within the G-proteinbinding domain of the rainbow trout b 2 -adrenoceptor sequence suggests that the in vivo control of trout b 2 -adrenoceptor signaling differs substantially from that of mammals.

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Section: Methodsmentioning

confidence: 99%

“…Fish were killed by a blow to the head and the liver quickly excised. Semi-purified membranes were prepared using a modified method developed for chick hearts [22]. The homogenate was centrifuged for 10 min at 10 8C and 2000 g (Sorvall RG-5B plus, SS 34 rotor).…”

Section: Membrane Preparationmentioning

confidence: 99%

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

Nickerson

Dugan

Drouin

et al. 2001

European Journal of Biochemistry

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“…The direction, called functional, used by this trajectory could be in actual fact an ontogenic or phylogenic direction. This accords with a study of membrane function in chicken cardiocytes, from one day after fertilisation of the egg until the bird was four months old (Daveloose et al, 1993). Generally speaking, like a mountaineer climbing a mountain by opening up a new route, the investment process consists in improving the level of the initial potential, thus creating the opportunity for new functional possibilities.…”

Section: Conclusion Biological Functionmentioning

confidence: 52%

Generalised Biological Function

Viret

2005

Acta Biotheor

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A physiological function can be described as a cycle based on a cusp bifurcation set in catastrophe theory. This cycle involves four phases that are successively developed along a functional potential, which is used to perform a given physiological act. The work we present is firstly based on a detailed study of the global function of vision, which covers a vast field extending from the molecular to cerebral scale. We then present other examples of generalised functions by expanding the frame of reference, from the scale of an organ to the scale of a whole organism with predation function, and to the scale of a social group with crisis management. We observed that the criteria remain the same, whether the function is considered on the scale of a cell membrane or a group of individuals organised into a cohort. Moreover, a so-called generalised function can be broken down into simpler sub-functions, each phase of the initial function containing the physiological act of each sub-function. It seems thus that a fractal extension may characterise the four-cusp spiral. This spiral is increasingly large as the types of sub-functions are multiplied. In functions relative to sociological science, such as crisis management, which are not natural but built and designed by humans, close attention must be paid to following the rules of these generalised biological functions.

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“…Plasma membranes from intestine were purified by density gradient centrifugation (Daveloose et al, 1993). In brief, samples were pooled into three groups (each group pooled from 7-8 samples) and homogenized in isolation medium (300mmoll -1 sucrose, 10mmoll…”

Section: Gut Membrane Fluiditymentioning

confidence: 99%

Mechanisms of transepithelial ammonia excretion and luminal alkalinization in the gut of an intestinal air-breathing fish, Misgurnus anguilliacaudatus

Wilson

Moreira‐Silva

Delgado

et al. 2012

Journal of Experimental Biology

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-1). Carbonic anhydrase does not appear to be associated with gut alkalinization under these conditions as ethoxzolamide was without effect on J base . Membrane fluidity of the posterior intestine was low, suggesting low permeability, which was also reflected in a lower mucosal-serosal J amm in the presence of an imposed gradient, in contrast to that in the anterior intestine. To conclude, although the posterior intestine is highly modified for gas exchange, it is the anterior intestine that is the likely site of ammonia excretion and alkalinization leading to ammonia volatilization in the gut. Supplementary material available online at

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Fluidity of Chicken Ventricular Plasma Membranes During Development in-ovo and after Birth: Spin Labelling and Fluorescence Studies

Cited by 5 publications

References 0 publications

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

Generalised Biological Function

Mechanisms of transepithelial ammonia excretion and luminal alkalinization in the gut of an intestinal air-breathing fish, Misgurnus anguilliacaudatus

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Fluidity of Chicken Ventricular Plasma Membranes During Development in-ovo and after Birth: Spin Labelling and Fluorescence Studies

Cited by 5 publications

References 0 publications

A putative β2‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

A putative β2‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

Generalised Biological Function

Mechanisms of transepithelial ammonia excretion and luminal alkalinization in the gut of an intestinal air-breathing fish, Misgurnus anguilliacaudatus

Contact Info

Product

Resources

About

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)