Fluidity of biodegradable substrate regulates carcinoma cell behavior: A novel approach to cancer therapy

Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5

“…Four-branched copolymers poly(ε-caprolactone- co -D,L,lactide) (P(CL- co -DLLA) were synthesized as described in our earlier reports 14 , 15 . The structure and molecular weights were determined by 1 H NMR spectroscopy (JEOL, Tokyo, Japan) and gel permeation chromatography (GPC; JASCO International, Tokyo, Japan) respectively.…”

“…The viscoelastic spectrum (storage modulus, G' and loss modulus, G'') of the substrate was tested as a function of frequency and temperature using a rheometer (MCR 301, Anton Paar, Tokyo, Japan). The non-crosslinked P(CL- co -DLLA) substrate for cell culture was prepared by a spin-coating technique as described in our previous report 14 . Crosslinked substrate was prepared by thermal crosslinking P(CL- co -DLLA) macromonomers as mentioned in our previous reports 14 , 15 .…”

“…The non-crosslinked P(CL- co -DLLA) substrate for cell culture was prepared by a spin-coating technique as described in our previous report 14 . Crosslinked substrate was prepared by thermal crosslinking P(CL- co -DLLA) macromonomers as mentioned in our previous reports 14 , 15 . The mechanical property of the crosslinked substrate was characterized by a tensile test (EZ-S 500N; Shimadzu, Kyoto, Japan).…”

Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds

“…16,17,19 Six types of poly(CL-co-DLLA) were characterized using the following measurements (Table 1): Thermal properties of poly(CL-co-DLLA) samples were characterized by DSC (DSC6100, Seiko Instruments, Chiba, Japan). Viscoelastic properties of poly(CL-co-DLLA) samples were tested at 37 C using a rheometer (MCR 301, Anton Paar, Tokyo, Japan) with parallel plate geometry (rotating top plate of 25 mm diameter).…”

“…We previously reported that viscoelastic and thermal properties of polymers affect cell adhesion and senescence. 16,17 To develop highly functionalized biomaterials, it is important to investigate the combinational effects of biomolecule properties and their immobilized polymer properties. We reported that cell-selective adhesion is significantly influenced by combining the physicochemical properties of peptides and polymers.…”