Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds

Kurimoto, Rio; Kanie, Kei; Uto, Koichiro; Kawai, Shun; Hara, Mitsuo; Nagano, Shusaku; Honda, Hiroyuki; Naito, Masanobu; Ebara, Mitsuhiro; Kato, Ryuji

doi:10.2116/analsci.32.1195

Cited by 6 publications

(5 citation statements)

References 26 publications

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“…A four-branched copolymer, ploy (ε-caprolactone-co-d,l-lactide) (P(CL-co-DLLA)) (CL:DLLA = 60:40), was synthesized by ring-opening copolymerization according to a previously described method [14,17,18]. The basic structure was evaluated by 1 H NMR (400 MHz) (Bruker Nano GmbH, Berlin, Germany).…”

Section: Materials Preparation and Releasing Assaymentioning

confidence: 99%

“…Combining meteorological models with classic ZTD models, such as those of Saastamoinen [5] and Hopfield [6], is commonly used as a ZTD modeling method when in situ meteorological data are difficult to collect. The main representative models are the University of New Brunswick (UNB) series models [7][8][9], the Global Pressure/Temperature (GPT) series models [10][11][12], the Improved Tropospheric Grid (ITG) models [13], and the Chinese Tropospheric Model (Ctrop) model [14]. With the continuous updating of reanalysis data in meteorological modeling, the accuracy and reliability of combined ZTD models can be further enhanced.…”

Section: Rna Sequence Analysismentioning

confidence: 99%

“…Previously, we developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices with high affinity to fibroblasts [14]. The ductile properties of polymer contents help to overcome the poor fracture toughness of HAp.…”

Section: Introductionmentioning

confidence: 99%

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Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

et al. 2020

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Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing pulp capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices. The polymer contents help overcome the poor fracture toughness of hydroxyapatite (HAp)-facilitated osteogenic differentiation of pulp cells. However, the composition of this novel polymer remained unclear. This study evaluated a novel polymer composite, P(CL-co-DLLA) and HAp, as a direct pulp capping carrier for biological molecules. The biocompatibility of the novel polymer composite was evaluated by determining the cytotoxicity and proliferation of human dental stem cells in vitro. The novel polymer composite with BMP-2, which reportedly induced tertiary dentin, was tested as a direct pulp capping material in a rat model. Cytotoxicity and proliferation assays revealed that the biocompatibility of the novel polymer composite was similar to that of the control. The novel polymer composite with BMP-2-induced tertiary dentin, similar to hydraulic calcium-silicate cement, in the direct pulp capping model. The BMP-2 composite upregulated wound healing-related gene expression compared to the novel polymer composite alone. Therefore, we suggest that novel polymer composites could be effective carriers for pulp capping.

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Section: Materials Preparation and Releasing Assaymentioning

confidence: 99%

Section: Rna Sequence Analysismentioning

confidence: 99%

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Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

et al. 2020

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“…Other physical properties such as modulus, morphological features, and thermal features can also dramatically influence cell adhesion and may promote selective cell adhesion in some cases. For example, fibroblasts, generally considered as tough cells that show good adhesion under various conditions, form spherical aggregates on an RGD-immobilized surface with a low modulus . Investigation into the mechanisms of cell sensing on nanoscaled surfaces can provide guidance for the fabrication of cell-selective surfaces as well. , …”

Section: Design and Fabrication Of Cell-selective Surfaces And Interf...mentioning

confidence: 99%

Design and Applications of Cell-Selective Surfaces and Interfaces

et al. 2018

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Tissue regeneration involves versatile types of cells. The accumulation and disorganized behaviors of undesired cells impair the natural healing process, leading to uncontrolled immune response, restenosis, and/or fibrosis. Cell-selective surfaces and interfaces can have specific and positive effects on desired types of cells, allowing tissue regeneration with restored structures and functions. This review outlines the importance of surfaces and interfaces of biomaterials with cell-selective properties. The chemical and biological cues including peptides, antibodies, and other molecules, physical cues such as topography and elasticity, and physiological cues referring mainly to interactions between cells-cells and cell-chemokines or cytokines are effective modulators for achieving cell selectivity upon being applied into the design of biomaterials. Cell-selective biomaterials have also shown practical significance in tissue regeneration, in particular for endothelialization, nerve regeneration, capture of stem cells, and regeneration of tissues of multiple structures and functions.

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“…In recent years, the development of tissue engineering has provided a new strategy for the repair of tissue injuries. The core of tissue engineering is to construct new tissue substitutes composed of biological materials and cells for promoting the recovery and maintenance of biological functions [ 1 , 2 ]. Biological materials not only offer three-dimensional space for cell adhesion, growth, and migration but also form adjustable microenvironments for the nutrition obtainment and waste excretion of cells [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Combining PLGA Scaffold and MSCs for Brain Tissue Engineering: A Potential Tool for Treatment of Brain Injury

Zhou

Fang

et al. 2018

Stem Cells International

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Nerve tissue engineering is an important strategy for the treatment of brain injuries. Mesenchymal stem cell (MSC) transplantation has been proven to be able to promote repair and functional recovery of brain damage, and poly (lactic-co-glycolic acid) (PLGA) has also been found to have the capability of bearing cells. In the present study, to observe the ability of PLGA scaffold in supporting the adherent growth of MSCs and neurons in vivo and vitro and to assess the effects of PLGA scaffold on proliferation and neural differentiation of MSCs, this study undertakes the following steps. First, MSCs and neurons were cultured and labeled with green fluorescent protein (GFP) or otherwise identified and the PLGA scaffold was synthesized. Next, MSCs and neurons were inoculated on PLGA scaffolds and their adhesion rates were investigated and the proliferation of MSCs was evaluated by using MTT assay. After MSCs were induced by a neural induction medium, the morphological change and neural differentiation of MSCs were detected using scanning electron microscopy (SEM) and immunocytochemistry, respectively. Finally, cell migration and adhesion in the PLGA scaffold in vivo were examined by immunohistochemistry, nuclear staining, and SEM. The experimental results demonstrated that PLGA did not interfere with the proliferation and neural differentiation of MSCs and that MSCs and neuron could grow and migrate in PLGA scaffold. These data suggest that the MSC-PLGA complex may be used as tissue engineering material for brain injuries.

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Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds

Cited by 6 publications

References 26 publications

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

Design and Applications of Cell-Selective Surfaces and Interfaces

Combining PLGA Scaffold and MSCs for Brain Tissue Engineering: A Potential Tool for Treatment of Brain Injury

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