Fluid secretion by the testis

Bp, Setchell

doi:10.1530/jrf.0.0140347

Cited by 21 publications

(14 citation statements)

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“…The secretion of rete testis fluid and the production of spermatozoa seem to be especially sensitive to even the short periods of ischaemia usual in setting up a perfusion. This and the fact that the secretion of fluid seems to be independent of perfusion pressure but dependent on glucose concentration is further evidence that the secretion of fluid by the seminiferous tubules is an active process, not passive filtration (Setchell, 1967a;Waites & Setchell, 1968).…”

Section: Discussionmentioning

confidence: 94%

Metabolism, sperm and fluid production of the isolated perfused testis of the sheep and goat

Linzell¹,

Setchell²

1969

The Journal of Physiology

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SUMMARY1. A total of nineteen ram and three goat testes have been perfused in isolation at 34-35°C for 3j-9 hr with heparinized blood and an added 5-HT antagonist (bromolysergic acid diethylamide) and their function compared with that of the normal ram testes in vtvo.2. The metabolism of the perfused testes and the testes in vivo was similar but blood flow through the perfused testes was two to three times normal.3. Vasoconstriction was produced by adrenaline and noradrenaline (10-20 /tg I.A.) and by electrical stimulation of nerves in the spermatic cord or of the lumbar sympathetic chain; these responses were abolished or reduced by phenoxybenzamine. 4. Flow of fluid from the rete testis continued only if ischaemia was reduced to a minimum and glucose concentration in the blood perfusing the testis was kept above about 25 mg/100 ml.; the fluid secreted during perfusion was of normal composition.5. The perfused testis showed no evidence of autoregulation and the flow of fluid was not affected by changes in perfusion pressure.6. When the temperature of three testes was raised to 40°C for 2 hr, metabolism increased but blood flow was unaltered; the flow of fluid and the concentration of spermatozoa decreased during heating.7. The testes perfused at normal scrotal temperatures (34-35' C) were histologically normal but some abnormalities were observed in the heated testes.

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Section: Discussionmentioning

confidence: 94%

Metabolism, sperm and fluid production of the isolated perfused testis of the sheep and goat

Linzell¹,

Setchell²

1969

The Journal of Physiology

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“…First, acetazolamide and 6_ethoxy-zolamide, inhibitors of carbonic anhydrase (CA) did not affect the Isc response to ATP (Table 1). Rete testis fluid collection following efferent duct ligation has shown no acute effect of acetazolamide on fluid production by the rat testis (Setchell, 1967). Using a histochemical method to detect CA, Cohen et al (1976) and Ekstedt & Ridderstrale (1992) localized CA in the interstitial cells but not in the seminiferous tubules of the rat and rabbit testes.…”

Section: Discussionmentioning

confidence: 99%

“…Although previous studies employing efferent duct ligation in intact rat testes (Setchell, 1967) and micropuncture and microcannulation of the rat seminiferous tubules in vivo (Tuck et al 1970;Levinè & Marsh, 1971) and in vitro (Cheung et al 1977) have provided evidence that the seminiferous tubules elaborate a watery secretion into the tubular lumen, the mechanism of secretion and its regulation have not been fully explored. This study represents a first attempt to unravel secretory mechanisms by measuring electrogenic ion transport across cultured monolayers of rat Sertoli cells.…”

Section: Discussionmentioning

confidence: 99%

Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats

Chan

Chew

et al. 1998

The Journal of Physiology

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Cultured epithelia of Sertoli cells from prepubertal rats were grown on Matrigel‐coated millipore filters for short‐circuit current (Isc) measurements. Under basal conditions, these epithelia exhibited a ‘zero’ transepithelial potential difference, a ‘zero’ short‐circuit current and a transepithelial resistance of 60 Ω cm2. Forskolin (100 μm) and 8‐(4‐chlorophenylthio)‐cAMP (cpt‐cAMP) (100 μm) added to the apical side stimulated the Isc (forskolin, peak ΔIsc= 1.32 ± 0.16 μA cm−1; cpt‐cAMP, peak ΔIsc= 0.88 ± 0.16 μA cm−2). ATP (100 μm) added apically elicited a Isc response (peak ΔIsc= 6.45 ± 0.28 μA cm−2) which was similar in magnitude to that of 1 μm thapsigargin (peak ΔIsc= 6.09 ± 0.44 μA cm−2). The potency of the responses to other nucleotides: UTP ≥ ATP > ADP >> AMP = adenosine indicates the involvement of a mixture of P2Y receptors. Removal of extracellular Cl− and HCO3− reduced the Isc response to ATP by 70 % and 40 %, respectively. Removal of K+ had no effect, whereas removal of Na+ attenuated the Isc response. The response to ATP was insensitive to agents known to block anion secretion (except apical diphenylamine‐2‐carboxylate (DPC) and DIDS). The resistance to perturbation by pharmacological agents may be a unique property of the seminiferous epithelium. Whole‐cell current recordings in cultured rat Sertoli cells demonstrated a DIDS‐sensitive outwardly rectifying Cl− conductance with activating and inactivating characteristics at depolarizing and hyperpolarizing voltages, respectively. The stimulation of electrogenic ion transport by ATP may be part of a complex mechanism regulating fluid secretion by the testis. Cultured Sertoli cell epithelia are shown to provide a useful model to investigate transepithelial transport in the seminiferous epithelium.

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“…Intravenous acetazolamide also reduced the flow of rete testis fluid in rams (Setchell, 1967;Setchell & Linzell, 1968), and it is difficult to explain why oral doses of the inhibitors did not also cause a reduction in flow since they should have been sufficient to give almost complete inhibition of carbonic anhydrase. Similar oral doses of inhibitors cause marked falls in the production of aqueous humour and cerebrospinal fluid (see Maren, 1967).…”

Section: Animalsmentioning

confidence: 99%

The Effect of Metabolic Alkalosis, Hypotension and Inhibitors of Carbonic Anhydrase on Fluid Secretion by Rat Testes

Setchell¹,

Brown²

1972

Reproduction

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Fluid secretion by the testis

Cited by 21 publications

References 0 publications

Metabolism, sperm and fluid production of the isolated perfused testis of the sheep and goat

Metabolism, sperm and fluid production of the isolated perfused testis of the sheep and goat

Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats

The Effect of Metabolic Alkalosis, Hypotension and Inhibitors of Carbonic Anhydrase on Fluid Secretion by Rat Testes

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