Fluid flow to mimic organ function in 3D <i>in vitro</i> models

Juste-Lanas, Yago; Hervas-Raluy, Silvia; Aznar, José Manuel García; González-Loyola, Alejandra

doi:10.1063/5.0146000

Cited by 8 publications

(2 citation statements)

References 332 publications

(329 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, this technology enables the precise delivery of reagents and the extraction of samples for subsequent analyses, all while preserving the integrity of the biological matrix. The efficacy of microfluidic platforms for LoC and OoC devices tailored to in vitro neuronal analyses has already been demonstrated [ 161 , 162 , 163 , 164 ].…”

Section: The Future Of Neurotransmitter Sensorsmentioning

confidence: 99%

Breaking Barriers: Exploring Neurotransmitters through In Vivo vs. In Vitro Rivalry

Lachance,

Gauvreau,

Boisselier

et al. 2024

Sensors

View full text Add to dashboard Cite

Neurotransmitter analysis plays a pivotal role in diagnosing and managing neurodegenerative diseases, often characterized by disturbances in neurotransmitter systems. However, prevailing methods for quantifying neurotransmitters involve invasive procedures or require bulky imaging equipment, therefore restricting accessibility and posing potential risks to patients. The innovation of compact, in vivo instruments for neurotransmission analysis holds the potential to reshape disease management. This innovation can facilitate non-invasive and uninterrupted monitoring of neurotransmitter levels and their activity. Recent strides in microfabrication have led to the emergence of diminutive instruments that also find applicability in in vitro investigations. By harnessing the synergistic potential of microfluidics, micro-optics, and microelectronics, this nascent realm of research holds substantial promise. This review offers an overarching view of the current neurotransmitter sensing techniques, the advances towards in vitro microsensors tailored for monitoring neurotransmission, and the state-of-the-art fabrication techniques that can be used to fabricate those microsensors.

show abstract

Section: The Future Of Neurotransmitter Sensorsmentioning

confidence: 99%

Breaking Barriers: Exploring Neurotransmitters through In Vivo vs. In Vitro Rivalry

Lachance,

Gauvreau,

Boisselier

et al. 2024

Sensors

View full text Add to dashboard Cite

show abstract

“…Bioreactors provide a constant nutrient supply while mimicking the natural cellular environment, enabling indirect monitoring of cellular behaviour in a non-invasive way. 18,19 There is a long history of bioreactor use in cartilage and bone engineering, showing that perfusion of culture medium at optimal flow rate can mimic the blood flow, as well as promote cell maturation and differentiation. 20 We have previously designed and described a bioreactor allowing long-term perfusion culture of whole rat liver ECM scaffold.…”

Section: Introductionmentioning

confidence: 99%

Rat liver extracellular matrix and perfusion bioreactor culture promote human amnion epithelial cell differentiation towards hepatocyte-like cells

Campinoti,

Almeida,

Goudarzi

et al. 2023

J Tissue Eng

View full text Add to dashboard Cite

Congenital and chronic liver diseases have a substantial health burden worldwide. The most effective treatment available for these patients is whole organ transplantation; however, due to the severely limited supply of donor livers and the side effects associated with the immunosuppressive regimen required to accept allograft, the mortality rate in patients with end-stage liver disease is annually rising. Stem cell-based therapy aims to provide alternative treatments by either cell transplantation or bioengineered construct transplantation. Human amnion epithelial cells (AEC) are a widely available, ethically neutral source of cells with the plasticity and potential of multipotent stem cells and immunomodulatory properties of perinatal cells. AEC have been proven to be able to achieve functional improvement towards hepatocyte-like cells, capable of rescuing animals with metabolic disorders; however, they showed limited metabolic activities in vitro. Decellularised extracellular matrix (ECM) scaffolds have gained recognition as adjunct biological support. Decellularised scaffolds maintain native ECM components and the 3D architecture instrumental of the organ, necessary to support cells’ maturation and function. We combined ECM-scaffold technology with primary human AEC, which we demonstrated being equipped with essential ECM-adhesion proteins, and evaluated the effects on AEC differentiation into functional hepatocyte-like cells (HLC). This novel approach included the use of a custom 4D bioreactor to provide constant oxygenation and media perfusion to cells in 3D cultures over time. We successfully generated HLC positive for hepatic markers such as ALB, CYP3A4 and CK18. AEC-derived HLC displayed early signs of hepatocyte phenotype, secreted albumin and urea, and expressed Phase-1 and -2 enzymes. The combination of liver-specific ECM and bioreactor provides a system able to aid differentiation into HLC, indicating that the innovative perfusion ECM-scaffold technology may support the functional improvement of multipotent and pluripotent stem cells, with important repercussions in the bioengineering of constructs for transplantation.

show abstract

Unveiling the Impact of Hemodynamics on Endothelial Inflammation‐Mediated Hepatocellular Carcinoma Metastasis Using a Biomimetic Vascular Flow Model

He,

Duan,

Liu

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Distant metastasis of hepatocellular carcinoma (HCC) mediated by hematogenous dissemination is a leading cause of HCC‐related deaths. The inflammatory response of vascular endothelial cells facilitates this process by promoting the adhesion and invasion of tumor cells in the circulatory system. While the regulation of this inflammation‐mediated metastasis through upregulation of adhesion molecules and disruption of intercellular junctions has been well established, the contribution of hemodynamics to this process remains poorly understood due to the lack of a suitable vascular flow model for investigation. In this study, we developed a vascular flow model with shear stress ranging from 0–6 dyn/cm2, which can mimic physiological blood flow conditions of small and medium vessels, to examine the impact of hemodynamics on endothelial inflammation‐mediated HCC metastasis. We found a significant negative correlation between HCC cells adhesion and shear stress loading during the fluid perfusion, the increasing shear stress will reduce the recruitment of HCC cells by disturbing adhesion forces between endothelium and HCC cells. However, this reduction will be restored by the inflammation. When applying high FSS (4‐6 dyn/cm2) to the inflammatory endothelium, there will be a 4.8‐fold increase in HCC cell adhesions compared to normal condition. Nevertheless, the increase fold of cell adhesions is inapparent, around 1.5‐fold, with low and medium FSS. This effect can be attributed to the FSS‐induced upregulation of ICAM‐1 and VCAM‐1 of the inflammatory endothelium, which serve to strengthen cell binding forces. The suppression of adhesion‐related factors by K7174 hinders the recruitment ability of vascular endothelial cells towards HepG2 cells. These findings indicate that hemodynamics plays a key role in HCC metastasis during endothelial inflammation by regulating the expression of adhesion‐related factors.This article is protected by copyright. All rights reserved

show abstract

Fluid flow to mimic organ function in 3D in vitro models

Cited by 8 publications

References 332 publications

Breaking Barriers: Exploring Neurotransmitters through In Vivo vs. In Vitro Rivalry

Breaking Barriers: Exploring Neurotransmitters through In Vivo vs. In Vitro Rivalry

Rat liver extracellular matrix and perfusion bioreactor culture promote human amnion epithelial cell differentiation towards hepatocyte-like cells

Unveiling the Impact of Hemodynamics on Endothelial Inflammation‐Mediated Hepatocellular Carcinoma Metastasis Using a Biomimetic Vascular Flow Model

Contact Info

Product

Resources

About