Fluid flow induced calcium response in osteoblasts: Mathematical modeling

Su, Jiali; Xu, Feng; Lü, Xin; Lu, Tian Jian

doi:10.1016/j.jbiomech.2011.05.004

Cited by 10 publications

(4 citation statements)

References 39 publications

(54 reference statements)

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“…In previous studies, glass slides were often employed as a substrate to investigate the effect of FSS magnitudes on osteoblasts. It was found that FSS min is 6 dyes/cm 2 and FSS opt is 10–15 dynes/cm 2 . In this study, glass slides (Glass) were employed as a control surface chemistry.…”

Section: Discussionmentioning

confidence: 99%

Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluid shear stress

Wang

Xing

et al. 2016

J. Biomed. Mater. Res.

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Scaffolds provide a physical support for osteoblasts and act as the medium to transfer mechanical stimuli to cells. To verify our hypothesis that the surface chemistry of scaffolds regulates the perception of cells to mechanical stimuli, the sensitivity and tolerability of osteoblasts to fluid shear stress (FSS) of various magnitudes (5, 12, 20 dynes/cm ) were investigated on various surface chemistries (-OH, -CH , -NH ), and their follow-up effects on cell proliferation and differentiation were examined as well. The sensitivity was characterized by the release of adenosine triphosphate (ATP), nitric oxide (NO) and prostaglandin E (PGE ) while the tolerability was by cellular membrane integrity. The cell proliferation was characterized by S-phase cell fraction and the differentiation by ALP activity and ECM expression (fibronectin and type I collagen). As revealed, osteoblasts demonstrated higher sensitivity and lower tolerability on OH and CH surfaces, yet lower sensitivity and higher tolerability on NH surfaces. Observations on the focal adhesion formation, F-actin organization and cellular orientation before and after FSS exposure suggest that the potential mechanism lies in the differential control of F-actin organization and focal adhesion formation by surface chemistry, which further divergently mediates the sensitivity and tolerability of ROBs to FSS and the follow-up cell proliferation and differentiation. These findings are essentially valuable for design/selection of desirable surface chemistry to orchestrate with FSS stimuli, inducing appropriate cell responses and promoting bone formation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2978-2991, 2016.

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Section: Discussionmentioning

confidence: 99%

Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluid shear stress

Wang

Xing

et al. 2016

J. Biomed. Mater. Res.

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“…It is well established that osteoblasts, including UMR106 osteoblast-like cells, are mechano-responsive with fluid flow inducing a rapid influx of Ca 2+ across the plasma membrane (Ban et al, 2011; Hung et al, 1995; Ishihara et al, 2013; Jing et al, 2013; Su et al, 2011; Suzuki et al, 2013; Thompson et al, 2012; Xing et al, 2011; You et al, 2001; Zhang et al, 2006). We validated the FFD by comparing fluid flow-induced Ca 2+ influx with this device to that obtained with the widely used Ibidi chamber; a commercially available device that allows biologically relevant laminar flow derived shear stress on cells cultured within the chambers.…”

Section: Resultsmentioning

confidence: 99%

Novel multi-functional fluid flow device for studying cellular mechanotransduction

Lyons

Iyer

Lovering

et al. 2016

Journal of Biomechanics

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Cells respond to their mechanical environment by initiating multiple mechanotransduction signaling pathways. Defects in mechanotransduction have been implicated in a number of pathologies; thus, there is need for convenient and efficient methods for studying the mechanisms underlying these processes. A widely used and accepted technique for mechanically stimulating cells in culture is the introduction of fluid flow on cell monolayers. Here, we describe a novel, multifunctional fluid flow device for exposing cells to fluid flow in culture. This device integrates with common lab equipment including routine cell culture plates and peristaltic pumps. Further, it allows the fluid flow treated cells to be examined with outcomes at the cell and molecular level. We validated the device using the biologic response of cultured UMR-106 osteoblast-like cells in comparison to a commercially available system of laminar sheer stress to track live cell calcium influx in response to fluid flow. In addition, we demonstrate the fluid flow-dependent activation of phospho-ERK in these cells, consistent with the findings in other fluid flow devices. This device provides a low cost, multi-functional alternative to currently available systems, while still providing the ability to generate physiologically relevant conditions for studying processes involved in mechanotransduction in vitro.

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“…We will see that this model reproduces the Ca 2+ homeostasis both in the presence and absence of IP 3 . Thus, the rate of change of the cytoplasmic Ca 2+ due to the SOC is written as [39]…”

Section: Intracellular Calciummentioning

confidence: 99%

“…includes three important experimental observations: (1) the probability of SOC opening, (2) the fact that the amount of Ca 2+ influx depends on the concentration gradient across the PM, and (3) the observation of no Ca 2+ influx at resting conditions. The proposed model for SOC [39] was modified by adding the last term in Equation ?? to satisfy the third observation.…”

Section: Intracellular Calciummentioning

confidence: 99%

Mathematical modeling of intracellular calcium in presence of receptor: a homeostatic model for endothelial cell

Nayak

Gou

Das

et al. 2022

Biomech Model Mechanobiol

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Calcium is a ubiquitous molecule and second messenger that regulates many cellular functions ranging from exocytosis to cell proliferation at different time scales. In the vasculature, a constant adenosine triphosphate (ATP) concentration is maintained because of ATP released by red blood cells (RBCs). These ATP molecules continuously react with purinergic receptors on the surface of endothelial cells (ECs). Consequently, a cascade of chemical reactions are triggered that result in a transient cytoplasmic calcium (Ca 2+ ), followed by return to its basal concentration. The mathematical models proposed in literature are able to reproduce the transient peak. However, the trailing concentration is always higher than the basal cytoplasmic Ca 2+ concentrations, and the Ca 2+ concentration in endoplasmic reticulum (ER) remains lower than its initial concentration. This means that the intracellular homeostasis is not recovered. We propose, herein, a minimal model of calcium kinetics. We find that the desensitization of EC surface receptors due to phosphorylation and recycling plays a vital role in maintaining calcium homeostasis in the presence of a constant stimulus (ATP). The model is able to capture several experimental observations such as refilling of Ca 2+ in the ER, variation of cytoplasmic Ca 2+ transient peak in ECs, the resting cytoplasmic Ca 2+ concentration, the effect of removing ATP from the plasma on Ca 2+ homeostasis, and the saturation of cytoplasmic Ca 2+ transient peak with increase in ATP concentration. Direct confrontation with several experimental results is conducted.This work paves the way to systematic studies for coupling between blood flow and chemical signaling, and should contribute to a better understanding of the relation between (patho)physiological conditions and Ca 2+ kinetics.

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Fluid flow induced calcium response in osteoblasts: Mathematical modeling

Cited by 10 publications

References 39 publications

Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluid shear stress

Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluid shear stress

Novel multi-functional fluid flow device for studying cellular mechanotransduction

Mathematical modeling of intracellular calcium in presence of receptor: a homeostatic model for endothelial cell

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