Fluid Dynamics and Biofilm Removal Generated by Syringe-delivered and 2 Ultrasonic-assisted Irrigation Methods: A Novel Experimental Approach

Layton, Gillian; Wu, Wen-I; Selvaganapathy, P. Ravi; Friedman, Shimon; Kishen, Anil

doi:10.1016/j.joen.2015.01.027

Cited by 53 publications

(49 citation statements)

References 34 publications

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“…, Layton et al . ). The canals of bovine teeth were covered with mucin to provide an additional protein platform for the biofilm (Lundstrom et al .…”

Section: Root Canal Biofilm Model Systems: Factors To Considermentioning

confidence: 97%

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Biofilm model systems for root canal disinfection: a literature review

et al. 2018

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The aim of this review was to present an overview of laboratory root canal biofilm model systems described in the endodontic literature and to critically appraise the various factors that constitute these models. The electronic databases MEDLINE, Web of Science and EMBASE were searched up to and including December 2016 to identify laboratory studies using endodontic biofilm models. The following search terms were used in various combinations: biofilm, root canal, in vitro, endodontic, bacteria, root canal infection model, colony‐forming unit. Only English papers from journals with an impact factor were selected. The records were screened by two reviewers, and full‐text articles were assessed according to pre‐defined criteria. The following data were extracted from the included studies: the microbial composition of the biofilm, the substrate, growth conditions, validation and quantification. Seventy‐seven articles met the inclusion criteria. In the majority (86%) of the studies, a monospecies biofilm was cultured. In two studies, a dual‐species biofilm was grown; others cultivated a multispecies biofilm, containing at least three species. Enterococcus faecalis was the most frequently used test species (in 79% of all studies, 92% of the monospecies studies). Four studies used an inoculum derived directly from the oral cavity. Human dentine was the most frequently used substratum (88% of the studies). Incubation times differed considerably, ranging from one to seventy days. The most common quantification method (in 87% of the studies) was bacterial culturing, followed by microscopy techniques. The variation in laboratory root canal biofilm model systems is notable. Because of substantial variation in experimental parameters, it is difficult to compare results between studies. This demonstrates the need for a more standardized approach and a validated endodontic biofilm model.

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“…, Layton et al . ). The canals of bovine teeth were covered with mucin to provide an additional protein platform for the biofilm (Lundstrom et al .…”

Section: Root Canal Biofilm Model Systems: Factors To Considermentioning

confidence: 97%

“…Layton et al . () selected polyethylene glycol (PEG)‐modified polydimethylsiloxane (PDMS) as substrate material.…”

Section: Root Canal Biofilm Model Systems: Factors To Considermentioning

confidence: 99%

Biofilm model systems for root canal disinfection: a literature review

et al. 2018

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“…These mechanisms are responsible for a greater reduction of the intracanal biofilm 23,24) . The ultrasonic application mode might be intermittent or continuous.…”

Section: Introductionmentioning

confidence: 99%

“…Continuous ultrasonic irrigation (CUI) is delivered via an irrigation source that is connected to the ultrasonic needle. It showed effective irrigation dynamics with continuous replacement of irrigant and keeping the canal filled with irrigant 24) . Commercially, the VPro StreamClean system (Vista Dental Products, Racine, WI, USA) is one of the CUI system available with an ultrasonic 30-G needle which is made of nickeltitanium alloy.…”

Section: Introductionmentioning

confidence: 99%

Irrigation effectiveness of continuous ultrasonic irrigation system: An <i>ex vivo</i> study

Jamleh

Suda

Adorno

2018

Dental Materials Journal

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This study evaluated the irrigation extrusion and smear layer (SL) removal of continuous ultrasonic irrigation (CUI). Forty root canals were shaped, embedded in warm sterile saline agar colored with 1% acid red, and distributed into four groups; three groups had the final irrigation sequence of sodium hypochlorite and EDTA solutions performed by using syringe irrigation (SI), EndoVac (EV), and CUI, respectively. Group 4 had the canals ultrasonically irrigated with saline (CUIS). Irrigant extrusion was verified by a color change of the agar. The SL was evaluated under scanning electron microscope. Irrigant extrusion was observed in two and four teeth in the CUI and SI, respectively, whilst none had irrigant extruded in the EV. In term of SL, the CUI was comparable to EV at 1 and 3 mm but different to SI at 1 mm. Although CUI might clean the root canal system effectively, it was unable to avoid irrigant extrusion.

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“…Although recent evidence suggested that the efficacy of NaOCl could be enhanced by irrigant agitation (Layton et al . , Mohmmed et al . ,b) or by increasing its concentration (Mohmmed et al .…”

Section: Introductionmentioning

confidence: 99%