Flubendiamide Enhances Adipogenesis and Inhibits AMPKα in 3T3-L1 Adipocytes

Abstract

Flubendiamide, a ryanoid class insecticide, is widely used in agriculture. Several insecticides have been reported to promote adipogenesis. However, the potential influence of flubendiamide on adipogenesis is largely unknown. The current study was therefore to determine the effects of flubendiamide on adipogenesis utilizing the 3T3-L1 adipocytes model. Flubendiamide treatment not only enhanced triglyceride content in 3T3-L1 adipocytes, but also increased the expression of cytosine-cytosine-adenosine-adenosine-thymidine (CCAAT)/enhancer-binding protein α and peroxisome proliferator-activated receptor gamma-γ, two important regulators of adipocyte differentiation. Moreover, the expression of the most important regulator of lipogenesis, acetyl coenzyme A carboxylase, was also increased after flubendiamide treatment. Further study revealed that 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) or A769662, two Adenosine 5′-monophosphate (AMP)-activated protein kinase α activators, subverted effects of flubendiamide on enhanced adipogenesis. Together, these results suggest that flubendiamide promotes adipogenesis via an AMPKα-mediated pathway.

Amelioration of oxidative stress-mediated cytotoxicity and genotoxicity induced by copper and flubendiamide in-vivo and in- vitro by potent antioxidants

“…The results of this study showed increased ROS levels and decreased GSH content and reduction in the activities of SOD and GST while increase in GPx activity. Present ndings are in line with the previous toxicity studies of copper and copper nanoparticles 84,85,67,77,72 and ubendiamide toxicity studies [86][87][88] in liver and kidneys.…”

The Impact of Polyphenol on General Nutrient Metabolism in the Monogastric Gastrointestinal Tract

“…It was observed via the activation of AMPK (adenosine monophosphate-activated protein kinase) that curcumin impeded the synthesis of glycerol lipids by hindering the activity of (GPAT-1) glycerol-3-phosphate acyl transferase-1, which in turn esterifies fatty acids into glycerol to form triglycerides for storage [93]. Peng et al as well affirmed in recent research that vitexin, i.e., a flavonoid glycoside (an apigenin-8-C-glucoside), activated AMPKα, which is a key enzyme in controlling and reducing fat accumulation [94][95][96]. Other animal and cell culture studies revealed that the addition of a phenolic compound (resveratrol) to rat isolated hepatocytes turns to hinder the synthesis of triglycerides and fatty acid, i.e., through the lowering effect of lipids.…”

Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole

“…The method for culture 3T3-L1 preadipocytes was determine based on previous studies ( 9 , 16 ). 3T3-L1 preadipocytes were maintained at 37°C in a carbon dioxide incubator in DMEM containing 10% FBS.…”

“…More and more studies are reporting the potential link between pesticides exposure and obesity (9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Our previous study has shown that chlorantraniliprole may enhance adipogenesis in 3T3-L1 adipocytes through inhibiting AMPK (19).…”