Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole

Abstract

IntroductionChlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the molecular mechanisms of chlorantraniliprole-induced fat accumulation in 3T3-L1 adipocytes.MethodsWe measured the triglyceride content in chlorantraniliprole-treated 3T3-L1 adipocytes, and collected cell samples treated with chlorantraniliprole for 24 h and without any treatment for RNA sequencing.ResultsCompared with the control group, the content of triglyceride in the treatment group of chlorantraniliprole was significantly increased. The results of RNA sequencing (RNA-seq) showed that 284 differentially expressed genes (DEGs) were identified after treatment with chlorantraniliprole, involving 39 functional groups of gene ontology (GO) and 213 KEGG pathways. Moreover, these DEGs were significantly enriched in several key genes that regulate adipocyte differentiation and lipogenesis including Igf1, Rarres2, Nr1h3, and Psmb8.DiscussionIn general, these results suggest that chlorantraniliprole-induced lipogenesis is attributed to a whole-gene transcriptome response, which promotes further understanding of the potential mechanism of chlorantraniliprole-induced adipogenesis.

The fat accumulation promotion effects of dihydrxytetraphenylmethane and its underlying mechanisms via transcriptome analysis

“…3T3L-1 cells differentiated for 24 h, 48 h, 96 h and 8 d were completely digested with Trizol reagent and collected. Total RNA of the samples was extracted without RNase and cDNA libraries were synthesized using the Revert-Aid First Strand cDNA Synthesis Kits as previously reported ( Chen et al, 2022 ; Qi et al, 2022 ). The RT-qPCR was then conducted on a StepOne Plus real-time PCR system.…”

A Rapid and Sensitive Gold Nanoparticle-Based Lateral Flow Immunoassay for Chlorantraniliprole in Agricultural and Environmental Samples

“…After adding 17 g of water, the mixture was filtered and recrystallized using ethanol to obtain the compound 2-[3-bromo-1-(3-chloro-2-pyridinyl)-1H-5-pyrazol]-6-chloro-8-methyl-4H-benzo [d]. A total of 0.26 mmol of aminobutyric acid and 0.45 mmol of NaOH were added to 5 mL of anhydrous DMF in a 25 mL round-bottomed flask and stirred at 25 [1,3] oxazin-4-one was added. After stirring for 12 h, the solution was mixed with 15 mL of water, adjusted to a pH of 3 using concentrated hydrochloric acid, and then extracted three times with 5 mL of ethyl acetate.…”

“…Recrystallization with hexane and ethyl acetate (3:1, v/v) produced 80 mg of CAP hapten in 64.7% yield, which was characterized by high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance hydrogen spectroscopy ( 1 H NMR). [1,3] oxazin-4-one was added. After stirring for 12 h, the solution was mixed with 15 mL of water, adjusted to a pH of 3 using concentrated hydrochloric acid, and then extracted three times with 5 mL of ethyl acetate.…”

“…However, researchers found that beneficial insects could be harmed by eating nectar treated with CAP residue [2]. In addition, CAP has been proven to promote obesity and cardiac problems by affecting crucial obesity genes and disrupting protein secondary structures [3]. Many countries have regulated the residues of CAP in agricultural products with maximum residue limits (MRLs) to ensure food safety and human health.…”

Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

“…Mouse experiments have demonstrated that long-term exposure to CP (18 months) leads to hepatocyte hypertrophy and increased liver weight [11] . Furthermore, a recent study revealed that CP induces adipogenesis in 3T3-L1 adipocytes through the AMP-activated protein kinase α (AMPKα) pathway [12,13] . However, to the best of our knowledge, there are no reports on the effect of CP exposure on obesity or intestinal flora in vivo.…”