Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference

Fukusaki, Eiichiro; Kawasaki, Kengo; Kajiyama, Shin’ichiro; An, Chung-Il; Suzuki, Ken-ichi; Tanaka, Yoshikazu; Kobayashi, Akio

doi:10.1016/j.jbiotec.2004.02.019

Cited by 114 publications

(61 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The homologous transgene-induced PTGS of CHS resulting in white flowers is also reported in petunia (Napoli et al 1990;Van Der Krol et al 1990). Furthermore, when RNA interference targeted to the 3 0 untranslated region of a CHS was induced in Trenia plants, which have at least two CHS, gene-specific silencing was observed and the other CHS was not suppressed, whereas when RNA interference was targeted to the relatively conserved CHS coding region, simultaneous PTGS was induced (Fukusaki et al 2004). In case of endogenous CHS siRNA, the yellow beans of G. max result from PTGS of CHS; PTGS was shown to occur in a number of CHS by high-throughput sequencing of small RNA (Kurauchi et al 2009;Tuteja et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In the seed coats of yellow soybean (Glycine max), which has nine CHS, all CHS are simultaneously silenced by post-transcriptional gene silencing (PTGS) (Kurauchi et al 2009;Tuteja et al 2009). As an example of a more aggressive gene suppression process, PTGS, RNA interference and virus-induced gene silencing are known to suppress multigene family members and redundant genes simultaneously, e.g., in polyploid species (Napoli et al 1990;Van Der Krol et al 1990;Lawrence and Pikaard 2003;Fukusaki et al 2004;De Paoli et al 2009;Jiang et al 2011). PTGS of CHS producing pure white flowers is observed in several horticultural crops, such as star-type cultivars of Petunia hybrida (Metzlaff et al 1997;Koseki et al 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia

Ohno

Hosokawa

Kojima

et al. 2011

Planta

View full text Add to dashboard Cite

Garden dahlias (Dahlia variabilis) are autoallooctoploids with redundant genes producing wide color variations in flowers. There are no pure white dahlia cultivars, despite its long breeding history. However, the white areas of bicolor flower petals appear to be pure white. The objective of this experiment was to elucidate the mechanism by which the pure white color is expressed in the petals of some bicolor cultivars. A pigment analysis showed that no flavonoid derivatives were detected in the white areas of petals in a star-type cultivar 'Yuino' and the two seedling cultivars 'OriW1' and 'OriW2' borne from a red-white bicolor cultivar, 'Orihime', indicating that their white areas are pure white. Semi-quantitative RT-PCR showed that in the pure white areas, transcripts of two chalcone synthases (CHS), DvCHS1 and DvCHS2 which share 69% nucleotide similarity with each other, were barely detected. Premature mRNA of DvCHS1 and DvCHS2 were detected, indicating that these two CHS genes are silenced post-transcriptionally. RNA gel blot analysis revealed that small interfering RNAs (siRNAs) derived from CHSs were produced in these pure white areas. By high-throughput sequence analysis of small RNAs in the pure white areas with no mismatch acceptance, small RNAs were mapped to two alleles of DvCHS1 and two alleles of DvCHS2 expressed in 'Yuino' petals. Therefore, we concluded that simultaneous siRNA-mediated post-transcriptional gene silencing of redundant CHS genes results in the appearance of pure white color in dahlias.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia

Ohno

Hosokawa

Kojima

et al. 2011

Planta

View full text Add to dashboard Cite

show abstract

“…RNAi has proven to be very efficient in interfering with gene expression in various plant systems such as Petunia hybrida, Arabidopsis (Arabidopsis thaliana), Papaver somniferum, Torenia hybrida, Coffea arabica, and rice (Oryza sativa; Stam et al, 1997;Chuang and Meyerowitz, 2000;Wesley et al, 2001;Stoutjesdijk et al, 2002;Allen et al, 2004;Fukusaki et al, 2004;Lee et al, 2004;Ogita et al, 2004;Miki et al, 2005). The wide use of this powerful technique reflects its ease of application and the possibilities for genome-wide reverse genetics.…”

mentioning

confidence: 99%

RNA Interference-Based Gene Silencing as an Efficient Tool for Functional Genomics in Hexaploid Bread Wheat

2006

View full text Add to dashboard Cite

Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gain-or lossof-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum aestivum), where most of the genes are present in at least three homoeologous copies and conventional insertional mutagenesis is not effective. We have introduced into bread wheat double-stranded RNA-expressing constructs containing fragments of genes encoding Phytoene Desaturase (PDS) or the signal transducer of ethylene, Ethylene Insensitive 2 (EIN2). Transformed plants showed phenotypic changes that were stably inherited over at least two generations. These changes were very similar to mutant phenotypes of the two genes in diploid model plants. Quantitative real-time polymerase chain reaction revealed a good correlation between decreasing mRNA levels and increasingly severe phenotypes. RNAi silencing had the same quantitative effect on all three homoeologous genes. The most severe phenotypes were observed in homozygous plants that showed the strongest mRNA reduction and, interestingly, produced around 2-fold the amount of small RNAs compared to heterozygous plants. This suggests that the effect of RNAi in hexaploid wheat is gene-dosage dependent. Wheat seedlings with low mRNA levels for EIN2 were ethylene insensitive. Thus, EIN2 is a positive regulator of the ethylene-signaling pathway in wheat, very similar to its homologs in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa). Our data show that RNAi results in stably inherited phenotypes and therefore represents an efficient tool for functional genomic studies in polyploid wheat.

show abstract

“…Aida et al (2000) generated transgenic T. fourunieri plants that had lighter flower colors than those of the host through sense and antisense suppression of the CHS or dihydroflavonol 4-reductase (DFR) gene and reported that sense and antisense genes gave different phenotypes. Fukuzaki et al (2004) reported that T. hybrida cv. Summerwave Blue has two CHS genes and that the transcription of the double-strand RNA of the 3Ј-non-coding sequence of one of them could specifically down-regulate that molecular species.…”

mentioning

confidence: 99%

RNAi suppression of the anthocyanidin synthase gene in Torenia hybrida yields white flowers with higher frequency and better stability than antisense and sense suppression

Nakamura

Fukuchi-Mizutani

Miyazaki

et al. 2006

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference

Cited by 114 publications

References 10 publications

Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia

Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia

RNA Interference-Based Gene Silencing as an Efficient Tool for Functional Genomics in Hexaploid Bread Wheat

RNAi suppression of the anthocyanidin synthase gene in Torenia hybrida yields white flowers with higher frequency and better stability than antisense and sense suppression

Contact Info

Product

Resources

About