2017
DOI: 10.1371/journal.pone.0180781
|View full text |Cite
|
Sign up to set email alerts
|

Flow velocity-driven differentiation of human mesenchymal stromal cells in silk fibroin scaffolds: A combined experimental and computational approach

Abstract: Mechanical loading plays a major role in bone remodeling and fracture healing. Mimicking the concept of mechanical loading of bone has been widely studied in bone tissue engineering by perfusion cultures. Nevertheless, there is still debate regarding the in-vitro mechanical stimulation regime. This study aims at investigating the effect of two different flow rates (vlow = 0.001m/s and vhigh = 0.061m/s) on the growth of mineralized tissue produced by human mesenchymal stromal cells cultured on 3-D silk fibroin … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

3
53
0

Year Published

2018
2018
2023
2023

Publication Types

Select...
6

Relationship

4
2

Authors

Journals

citations
Cited by 59 publications
(56 citation statements)
references
References 43 publications
3
53
0
Order By: Relevance
“…Computational models (e.g., computational fluid dynamics [CFD], multiphasic finite element [FE]) have been used for calculating the local WSS for a variety of applied flow rates 4‐8 . In most previous CFD analyses, the models merely included the empty scaffold geometries which were obtained either from computer aided design (CAD) or micro‐computer tomography (μCT) scanning images 7‐11 . It was assumed that cells were initially attached flatly onto the scaffold surface prior to ECM deposition with the underlying assumption that the solid volume of cells was negligible and would not affect the fluid flow comparing to that in the empty scaffold 7,9‐11 .…”
Section: Introductionmentioning
confidence: 99%
“…Computational models (e.g., computational fluid dynamics [CFD], multiphasic finite element [FE]) have been used for calculating the local WSS for a variety of applied flow rates 4‐8 . In most previous CFD analyses, the models merely included the empty scaffold geometries which were obtained either from computer aided design (CAD) or micro‐computer tomography (μCT) scanning images 7‐11 . It was assumed that cells were initially attached flatly onto the scaffold surface prior to ECM deposition with the underlying assumption that the solid volume of cells was negligible and would not affect the fluid flow comparing to that in the empty scaffold 7,9‐11 .…”
Section: Introductionmentioning
confidence: 99%
“…1B, panel a) (8,127). Fluid shear influences cell proliferation, differentiation, morphology, and function (30,114,(128)(129)(130)(131)(132)(133)(134)(135)(136)(137)(138)(139)(140). Models developed within the dynamic RWV environment experience excellent mass transfer of nutrients/wastes and exhibit enhanced structure, differentiation, function, and multicellular complexity relative to 2-D monolayers (11,80,(141)(142)(143)(144)(145)(146)(147)(148)(149)(150)(151)(152)(153)(154).…”
Section: Modeling the Microenvironment: 3-d Models For Infectious Dismentioning
confidence: 99%
“…The homogeneous porous media flow (HPMF) model, which considers the scaffold as a homogeneous permeable solid, has been used in recent studies (Kleinhans et al, ; Vetsch, Betts, Muller, & Hofmann, ) to encompass scaffold hydraulic properties (e.g., porosity and permeability) while reducing the complexity of the simulation. Figure a shows the shear stress distribution at the median transverse cut plane predicted by this model for a flowrate of 12 ml/min (Vetsch et al, ). Figure b shows the shear stress distribution in a similar scaffold generated by finite element analysis (FEA) based on the actual scaffold geometry for a lower flowrate of 0.3 ml/min.…”
Section: Obstacles In Defining Optimal Flow Effectsmentioning
confidence: 99%
“…Limit of the homogeneous porous media flow (HPMF) model. (a) Wall shear stress map at the transverse cut plane (50%) in an 8‐mm diameter salt‐leached scaffold (pore size 315–400 µm, porosity 55%) for a 12 ml/min flowrate, calculated with HPMF (from Vetsch et al, ). (b) Wall shear stress map at the transverse cut plane (50%) in an 8 mm diameter salt‐leached scaffold (pore size 250–425 µm, porosity 80%) for a 0.3 ml/min flowrate, calculated with finite element analysis based on CFD using the actual scaffold geometry (from Liu et al, )…”
Section: Obstacles In Defining Optimal Flow Effectsmentioning
confidence: 99%
See 1 more Smart Citation