Flow cytometry in the diagnosis of cancer

Órfão, Alberto; Ciudad, Juana; González, Marcos; López, Antonio; Abad, María del Mar; Bouza, J. I. Paz; Cruz, Juan Jesús; Alonso, Alberto Gómez; Miguel, Jesús F. San

doi:10.3109/00365519509090577

Cited by 30 publications

(17 citation statements)

References 39 publications

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“…DNA ploidy analysis was performed as previously described [38,39]. Briefly, cells were fixed and stained with a saturating concentration (40 μg/ml) of propidium iodide and 100 μg/ml of RNase.…”

Section: Methodsmentioning

confidence: 99%

Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR

Wang¹,

Zhang

et al. 2017

PLoS ONE

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The chicken is an important agricultural animal and model for developmental biology, immunology and virology. Excess fat accumulation continues to be a serious problem for the chicken industry. However, chicken adipogenesis and obesity have not been well investigated, because no chicken preadipocyte cell lines have been generated thus far. Here, we successfully generated two immortalized chicken preadipocyte cell lines through transduction of either chicken telomerase reverse transcriptase (chTERT) alone or in combination with chicken telomerase RNA (chTR). Both of these cell lines have survived >100 population doublings in vitro, display high telomerase activity and have no sign of replicative senescence. Similar to primary chicken preadipocytes, these two cell lines display a fibroblast-like morphology, retain the capacity to differentiate into adipocytes, and do not display any signs of malignant transformation. Isoenzyme analysis and PCR-based analysis confirmed that these two cell lines are of chicken origin and are free from inter-species contamination. To our knowledge, this is the first report demonstrating the generation of immortal chicken cells by introduction of chTERT and chTR. Our established chicken preadipocyte cell lines show great promise as an in vitro model for the investigation of chicken adipogenesis, lipid metabolism, and obesity and its related diseases, and our results also provide clues for immortalizing other avian cell types.

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Section: Methodsmentioning

confidence: 99%

Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR

Wang¹,

Zhang

et al. 2017

PLoS ONE

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“…The resulting DNA histograms were evaluated using SFIT mode. Measures of DNA index were obtained by dividing the modal fluorescence channel of the G 0 /G 1 peak of the abnormal cells by the modal fluorescence channel of the residual G 0 /G 1 normal cells present in the sample [37]. For the purposes of detection by ploidy analysis, aneuploidy was defined as a DNA index greater than 1.02 or less than 0.98.…”

Section: Dna Ploidy Analysismentioning

confidence: 99%

DNA ploidy analysis as an adjunct for the detection of relapse in B-lineage acute lymphoblastic leukemia

Kenney

Zieske

Rinder

et al. 2008

Leukemia & Lymphoma

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Detection of relapse in acute lymphoblastic leukemia (ALL) is essential for proper management. However, immunophenotypic detection of relapse by flow cytometry in B-lineage ALL can be confounded by several factors, including lack of a unique immunophenotype and modulation of aberrant phenotypes after treatment. We hypothesized that flow cytometric DNA ploidy analysis may detect relapse in aneuploid ALL cases that might be missed by flow immunophenotyping. We retrospectively studied ALL cases at our institution between 1991 and 2003 (n = 114). Aneuploid populations were present at diagnosis in 32% of all patients. Sixty-five percent of all patients had "normal" leukemic immunophenotypes, defined as being similar to normal precursor B-cells, while 35% had "aberrant" immunophenotypes with myeloid or T antigen co-expression. In ALL cases that were originally aneuploid, follow-up ploidy-analysis detected relapsed disease in all cases which were also detected by flow immunophenotyping, suggesting that ploidy analysis is highly sensitive for detecting ALL relapse. However, in 5 cases in which the diagnosis of relapse could not be reliably made by flow immunophenotyping, ploidy analysis successfully detected aneuploid cells, i.e., relapse, in all five; these included 3 patients with normal and 2 with aberrant original immunophenotypes. These results suggest that it may be beneficial to perform ploidy analysis as an adjunct to flow immunophenotyping in following patients with B-lineage ALL who demonstrate aneuploidy at diagnosis.

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“…Multiparametric flow cytometry is an effective, reliable and fast technique to characterize tumour cells and identify interindividual heterogeneity and subpopulations within one individual tumour. Using this method, the simultaneous characterization of cell surface antigens, intracellular cytokines, DNA content and apoptosis-associated markers can be used at a single cell level (Hollander and Loken 1988, Brons et al 1990, Stokke et al 1991, Orfao et al 1995, Prussin and Metcalfe 1995, Corver et al 1996, Jason and Larned 1997, Könemann et al 2000, 2003, Bacso and Eliason 2001, Rasola and Geuna 2001.…”

Section: Introductionmentioning

confidence: 99%

Time‐ and dose‐dependent changes of intracellular cytokine and cytokine receptor profile of Ewing tumour subpopulations under the influence of ionizing radiation

Könemann

Bölling

Malath

et al. 2003

International Journal of Radiation Biology

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The observed radiation-induced changes of cytokine and receptor profile are an indication for complex intercellular interactions in view of radioresistance-associated mechanisms between cell populations within one individual tumour. The observed heterogeneous response on radiation might have therapeutic implications for an individualized therapy based on combined radiation and cytokine modulation, defined by flow cytometric characterization of markers potentially informative for radioresistance.

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Flow cytometry in the diagnosis of cancer

Cited by 30 publications

References 39 publications

Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR

Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR

DNA ploidy analysis as an adjunct for the detection of relapse in B-lineage acute lymphoblastic leukemia

Time‐ and dose‐dependent changes of intracellular cytokine and cytokine receptor profile of Ewing tumour subpopulations under the influence of ionizing radiation

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