Flow-cytometry detection of fluorescent magnetic nanoparticle clusters increases sensitivity of dengue immunoassay

Sanjaya, Kiki; Ranzoni, Andrea; Hung, Jacky; Blaskovich, Mark A.T.; Watterson, Daniel; Young, Paul R.; Cooper, Matthew A.

doi:10.1016/j.aca.2020.02.007

Cited by 10 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…187 The synergic effect of MNPs and fluorescent polymer nanoparticles in flow cytometry allows the detection of 100 fold lower concentrations of DenV than polymer nanoparticles alone. 310 Core-shell morphologies constitute the most common class of MNP nanocomposites. MNPs can act as supports for depositing different types of nanomaterials.…”

Section: As Wellmentioning

confidence: 99%

“…In flow cytometry, fluorescence latex nanoparticles (200 nm) function as reporters and are responsible for reducing the DenV detection limit from 150 ng mL À1 to 2.5 ng mL À1 . 310 The intrinsically conducting polypyrrole nanowires shown in Fig. 20B (diameter: 70 nm; length: 1 mm) are conducive to aptamer loading and are capable of distinguishing attomolar concentrations of viral proteins in artificial saliva within 5 s. 450 The blue-emitting polydioctylfluorene dots exhibit theranostic properties due to the synchronous detection and blocking of influenza viruses in the presence of sialyl glycans.…”

Section: As Wellmentioning

confidence: 99%

“…187 The synergic effect of MNPs and fluorescent polymer nanoparticles in flow cytometry allows the detection of 100 fold lower concentrations of DenV than polymer nanoparticles alone. 310…”

Section: Inorganic Nanomaterials In Virus Sensing and Trackingmentioning

confidence: 99%

“…418 Hyper-branched polymers such as dendrimers improve the conjugation between the target and the sensing platform. 88 Polymeric nanoparticles are also used in nanocomposites to tune the properties of MNPs, 310 AuNPs, 419 CNTs, 124 and QDs. 88,420 Such nanocomposites exhibit a symbiotic combination of unique morphologies with ultrasensitivity and facile fabrication processes.…”

Section: Organic Nanomaterials In Virus Sensing and Trackingmentioning

confidence: 99%

See 3 more Smart Citations

Nanomaterials for virus sensing and tracking

Pirzada

Altıntaş

2022

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

Section: As Wellmentioning

confidence: 99%

Section: As Wellmentioning

confidence: 99%

“…187 The synergic effect of MNPs and fluorescent polymer nanoparticles in flow cytometry allows the detection of 100 fold lower concentrations of DenV than polymer nanoparticles alone. 310…”

Section: Inorganic Nanomaterials In Virus Sensing and Trackingmentioning

confidence: 99%

Section: Organic Nanomaterials In Virus Sensing and Trackingmentioning

confidence: 99%

See 2 more Smart Citations

Nanomaterials for virus sensing and tracking

Pirzada

Altıntaş

2022

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…analyte) and background signal. The combination of flow cytometry tests of cluster sizes and fluorescence intensity resulted in declining the background signal and the enhancement of sensitivity [ 122 ]. In Table 4 , the recent studies of plasmonic detection for DENV can be found.…”

Section: Dengue Virus (Denv)mentioning

confidence: 99%

Role of plasmonics in detection of deadliest viruses: a review

et al. 2021

View full text Add to dashboard Cite

Viruses have threatened animal and human lives since a long time ago all over the world. Some of these tiny particles have caused disastrous pandemics that killed a large number of people with subsequent economic downturns. In addition, the quarantine situation itself encounters the challenges like the deficiency in the online educational system, psychiatric problems and poor international relations. Although viruses have a rather simple protein structure, they have structural heterogeneity with a high tendency to mutation that impedes their study. On top of the breadth of such worldwide worrying issues, there are profound scientific gaps, and several unanswered questions, like lack of vaccines or antivirals to combat these pathogens. Various detection techniques like the nucleic acid test, immunoassay, and microscopy have been developed; however, there is a tradeoff between their advantages and disadvantages like safety in sample collecting, invasiveness, sensitivity, response time, etc. One of the highly resolved techniques that can provide early-stage detection with fast experiment duration is plasmonics. This optical technique has the capability to detect viral proteins and genomes at the early stage via highly sensitive interaction between the biological target and the plasmonic chip. The efficiency of this technique could be proved using commercialized techniques like reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) techniques. In this study, we aim to review the role of plasmonic technique in the detection of 11 deadliest viruses besides 2 common genital viruses for the human being. This is a rapidly moving topic of research, and a review article that encompasses the current findings may be useful for guiding strategies to deal with the pandemics . By investigating the potential aspects of this technique, we hope that this study could open new avenues toward the application of point-of-care techniques for virus detection at early stage that may inhibit the progressively hygienic threats.

show abstract