Flow cytometry: A new approach for the molecular profiling of breast cancer

Lostumbo, Antonella; Mehta, Divyesh; Setty, Suman; Núñez, Rafael

doi:10.1016/j.yexmp.2005.09.007

Cited by 40 publications

(29 citation statements)

References 27 publications

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“…78,79 The current criteria for detection and prognosis of breast cancer include an abnormal breast biopsy, tumor size, histological grade, estrogen and progesterone receptor status, and presence of the HER2/ Neu oncogene. 80,81 Breast cancer can also be diagnosed by detecting the various aberrantly methylated genes. MSP is currently being used to detect the methylation status of various genes in breast biopsy tissues samples.…”

Section: Dna Methylation and Breast Cancermentioning

confidence: 99%

DNA methylation in breast and colorectal cancers

2007

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DNA methylation is one of several epigenetic changes observed in cells. Aberrant methylation of tumor suppressor genes, proto-oncogenes, and vital cell cycle genes has led many scientists to investigate the underlying cellular mechanisms of DNA methylation under normal and pathological conditions. Although DNA methylation is necessary for normal mammalian embryogenesis, both hypo-and hypermethylation of DNA are frequently observed in carcinogenesis and other pathological disorders. DNA hypermethylation silences the transcription of many tumor suppressor genes, resulting in immortalization of tumor cells. The reverse process, demethylation and restoration of normal functional expression of genes, is augmented by DNA methylation inhibitors. Recent studies suggest that DNA hypomethylation may also control gene expression and chromosomal stability. However, the roles of and relationship between hypomethylation and hypermethylation are not well understood. This review provides a brief overview of the mechanism of DNA methylation, its relationship to extrinsic stimulation including dietary intake and aging, and of abnormally methylated DNA in breast and colorectal cancers, which could be used as prognostic and diagnostic markers.

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Section: Dna Methylation and Breast Cancermentioning

confidence: 99%

DNA methylation in breast and colorectal cancers

2007

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“…DEP has been a very successful technique for the manipulation, 23 separation, 24 and detection 25 of bioparticles. In contrast to techniques such as fluorescence activated cell sorting (FACS), 26 chemically functionalized pillar-based microchips, 27 and magnetic bead cell separation, 28 DEP does not require the use of target specific antibodies. Traditionally, DEP utilizes arrays of electrodes to produce non-uniform electric fields to polarize particles and thereby induce movement.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

et al. 2012

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Ovarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a nontumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool. V C 2012 American Institute of Physics. [http://dx

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“…Most widely used labeling-assisted methods are fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS) because of their high separation speed and specificity. 7 These cell sorting systems have been miniaturized to microfluidic devices, demonstrating the possibility of being integrated into lab-on-a-chip devices. 8,9 In contrast, label-free methods typically utilize differences in physical properties such as cell size, 10 density, 11 cell adhesion, [12][13][14][15] and dielectric properties.…”

Section: Introductionmentioning

confidence: 99%

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

et al. 2007

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A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e.g., one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 ml min 21 . The fraction of MCF7 cells was increased from 0.36 ¡ 0.04 to 0.83 ¡ 0.04 under these optimized conditions.

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Flow cytometry: A new approach for the molecular profiling of breast cancer

Cited by 40 publications

References 27 publications

DNA methylation in breast and colorectal cancers

DNA methylation in breast and colorectal cancers

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

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