Flow cytometric study on cell kinetics of brain tumours and their cultured cells

Abstract

With the aid of flow cytometry (FCM), distribution of DNA content in 40 cases of brain tumour, primary culture cell, and secondary culture cell can be determined and chronological change after subculture is studied from the analysis of their cell cycle. In most primary cultures, proliferating index (PI) is likely to decrease, which suggests that environmental change might affect the growth activity. In comparison with that of the original sample, DNA-histogram of the secondary culture can be divided into the following 3 types: the type recovering to the original pattern ("adapting type"), in which astrocytoma, ependymoma, glioblastoma and medulloblastoma are included, 2) the type increasing more at G2 + M phase than the original ("proliferating type"), in which meningioma and some of glioblastoma are included, and 3) the type decreasing so far as to induce degeneration or death ("degenerating type"), in which pituitary adenoma and neurinoma are included. FCM is of great usefulness for the study of cell kinetics of a tumour cell undergoing culture and the present method will be available for the respective study of biological characteristics of the cultured cell, established cell line or sensitivity test for antineoplastic agents.

Morphological Appearance, Growth Kinetics and Glial Fibrillary Acidic Protein (GFAP) Expression in Primary in Vitro Explant Culture of Astrocytic Neoplasms

“…Moreover, LIs of cultured anaplastic astrocytomas and glioblastomas have been found to be lower than the values obtained in cultures of low-grade astrocytomas. Our in vitro cell kinetic results are, however, in agreement with reports of other workers (20)(21)(22).…”

“…More recently, the estimation of various cell kinetic parameters have provided yet another index for more accurate malignancy assessment (6-12). However, very few studies are available on the morphological appearance ( 13-1 3 , GFAP expression (2, 13, [16][17][18][19] and growth kinetics (20)(21)(22)(23) of cultured human astrocytoma cells and no report, to the best of our knowledge, is available correlating all these parameters in vitro with the grade of malignancy of the parent tissue.…”

A sequential cell kinetic study of meningioma cells in primary explant culture using bromodeoxyuridine

“…2). This type of cell kinetic pattern of Group I cases showing a gradual reduction of LI was classified as 'degenerating' type [12]. All of the cases in this group had an LI of above 5 % at the end of first week in culture.…”

“…3). This type of growth pattern with a spurt in the second week LI followed by decline was designated as 'proliferating' type of pattern [12].…”

“…Recently, the in vitro BrdU-LI estimation at the end of first week in primary cultures of meningiomas has been shown to be on par [8] as an alternative to in vivo BrdU-LI [4,5] and DNA-FCM studies [6,7] for predicting the biological aggressiveness of these tumors irrespective of their histological homogeneity. Further, there are reports based on the DNA-FCM analysis which indicate that data obtained from primary cultures in meningiomas can provide equally valuable information for predicting biologic behaviour [9][10][11][12]. However, both the in vitro flow cytometric and BrdU-LI data on primary cultures is mostly limited to initial few days (upto 7 days) of culture, and sequential temporal data within the constraints of maximum permissible limits of maintaining primary culture are not available.…”