Morphological Appearance, Growth Kinetics and Glial Fibrillary Acidic Protein (GFAP) Expression in Primary in Vitro Explant Culture of Astrocytic Neoplasms

Kharbanda, Kusum K.; Sarkar, Chitra; Dinda, Amit Kumar; Karak, Asis Kumar; Mathur, Meera; Roy, S. C. Dutta

doi:10.3109/02841869309093599

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…slight SSX4 mRNA expression was observed on three glioma cell lines (U87MG, U138MG, Lab1) and GFAP mRNA expression was not observed on glioma cell lines except for Lab2 (data not shown). This last result is in accordance with previous studies which showed that a large number of glioma cell lines did not express GFAP at the protein level (Bigner et al 1981a;Westphal et al 1990;Bocchini et al 1991;Kharbanda et al 1993;Farr-Jones et al 1999).…”

Section: Resultssupporting

confidence: 95%

“…In the same manner, GFAP mRNA expression diminished after culture in three of the four glioma primary cultures and this diminution was observed in the four tested media (data not shown). This decrease has been observed at the protein level in other published studies (Westphal et al 1990;Bocchini et al 1991;Kharbanda et al 1993;Farr-Jones et al 1999).…”

Section: Resultssupporting

confidence: 59%

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Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy

Clavreul

Jean

Preisser

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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Immunotherapy, particularly active vaccination, may be developed as an effective and safe treatment modality for malignant gliomas, which continue to have a poor prognosis, despite advances in surgical techniques and adjuvant chemotherapy and radiotherapy. Since no glioma-specific tumor-associated antigens (TAAs) have been discovered, autologous tumor cells or well-established glioma cell lines could be used in future vaccination protocols to induce antitumour immunity against unknown TAAs. One obstacle for clinical use of these tumour cell vaccines is related to foetal calf serum (FCS). Efforts are currently being directed toward developing FCS-free media and serum-free alternatives to culture these cell vaccines. In this study, a medium containing human serum and one serum-free medium (UltraCulture), supplemented or not with epidermal growth factor, were tested on morphology, survival, DNA content and TAA expression of human glioma cell lines and glioma biopsy primary cultures. Their effects were compared on FCS-containing medium. Results show that, whatever the medium used, no significant variations in morphology and survival were observed. Furthermore, human serum-containing medium or UltraCulture preserved at early passage cultures the cell population of interest present in the biopsies before culture. In addition, the expression profile of eight TAAs was similar between these media. These data indicate that human serum-containing medium and UltraCulture serum-free medium could be promising candidates to produce tumour-cell vaccines.

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Section: Resultssupporting

confidence: 95%

Section: Resultssupporting

confidence: 59%

Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy

Clavreul

Jean

Preisser

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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“…Several coatings have been used and they have an impact on cellular fate (poly-(L)-lysine [ 99 ]; a film of chicken plasma [ 14 ]; poly-ornithine [ 100 ]; collagen, laminin, gelatin sponge foam or a Millipore filter [ 15 ]). Explants are usually cultivated in DMEM or in neurabasal serum-free media supplemented with N2 and B27 [ 101 ], or with FBS [ 15 , 98 , 102 , 103 ]. To better conserve GBM cellular heterogeneity, our team is currently using a stem-like cell medium supplemented with EGF, bFGF and B27.…”

Section: Organotypic Culturesmentioning

confidence: 99%

“…Throughout the culture, and depending on the medium used, differentiation can be observed. During the first 2 weeks an increase in GFAP staining and a decrease in the stem-like cell markers as A2B5 can be observed [ 99 , 103 , 104 ]. Then, in the second period of growth, cells become more differentiated into astrocytes, which co-express GFAP with vimentin, nestin or S-100 and even into oligodendrocytes [ 99 , 100 ].…”

Section: Organotypic Culturesmentioning

confidence: 99%

“…Then, in the second period of growth, cells become more differentiated into astrocytes, which co-express GFAP with vimentin, nestin or S-100 and even into oligodendrocytes [ 99 , 100 ]. Some authors described a dedifferentiation phase from the third week of culture and onward, with spindle and epithelioid cells becoming predominant [ 103 ]. Cell division by mitoses is commonly seen in the cultures [ 6 , 98 ].…”

Section: Organotypic Culturesmentioning

confidence: 99%

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Practical Review on Preclinical Human 3D Glioblastoma Models: Advances and Challenges for Clinical Translation

Soubéran

Tchoghandjian

2020

Cancers

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Fifteen years after the establishment of the Stupp protocol as the standard of care to treat glioblastomas, no major clinical advances have been achieved and increasing patient’s overall survival remains a challenge. Nevertheless, crucial molecular and cellular findings revealed the intra-tumoral and inter-tumoral complexities of these incurable brain tumors, and the essential role played by cells of the microenvironment in the lack of treatment efficacy. Taking this knowledge into account, fulfilling gaps between preclinical models and clinical samples is necessary to improve the successful rate of clinical trials. Since the beginning of the characterization of brain tumors initiated by Bailey and Cushing in the 1920s, several glioblastoma models have been developed and improved. In this review, we focused on the most widely used 3D human glioblastoma models, including spheroids, tumorospheres, organotypic slices, explants, tumoroids and glioblastoma-derived from cerebral organoids. We discuss their history, development and especially their usefulness.

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Tumors of the Glia: Recent Advances

Suri

Mahajan

Singh

et al. 2022

The Biology of Glial Cells: Recent Advances

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Morphological Appearance, Growth Kinetics and Glial Fibrillary Acidic Protein (GFAP) Expression in Primary in Vitro Explant Culture of Astrocytic Neoplasms

Cited by 6 publications

References 23 publications

Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy

Human glioma cell culture: two FCS-free media could be recommended for clinical use in immunotherapy

Practical Review on Preclinical Human 3D Glioblastoma Models: Advances and Challenges for Clinical Translation

Tumors of the Glia: Recent Advances

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