Flow Cytometric Quantification of Peripheral Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived Microparticles in Pulmonary Arterial Hypertension

Rose, Jonathan A.; Wanner, Nicholas; Cheong, Hoi I.; Queisser, Kimberly; Barrett, Patrick; Park, Margaret; Hite, Corrine; Prasad, Sathyamangla V. Naga; Erzurum, Serpil C.; Asosingh, Kewal

doi:10.1371/journal.pone.0156940

Cited by 20 publications

(25 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bristow et al showed loss of β 1 -ARs in the failing RV, depletion of tissue norepinephrine, and lower levels of adenylate cyclase activity at baseline and in response to stimulation, which were identical to findings in left heart failure (13). Another study recently confirmed these findings through identification of lesser numbers of βARs in circulating mononuclear cells in individuals with PAH as compared with healthy controls or those with asthma (15).…”

Section: L I N I C a L M E D I C I N Ementioning

confidence: 73%

Pulmonary arterial hypertension treatment with carvedilol for heart failure: a randomized controlled trial

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Section: L I N I C a L M E D I C I N Ementioning

confidence: 73%

Pulmonary arterial hypertension treatment with carvedilol for heart failure: a randomized controlled trial

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Multiple studies have described mononuclear/macrophage-derived EVs rich in inflammatory effector molecules like cytokines, chemokines, adhesion molecules and proteases to cause alveolar wall destruction and Urine EMPs [97] Group III PH Plasma ↑Tissue factor and endoglin in EMPs [98] Group IV PH Plasma ↑Tissue factor and endoglin in EMPs [98] Plasma ↑PMPs, LMPs and EMPs [99] Asthma Blood ↑Eosinophil derived exoxomes [100,101] BALF ↑ CD63 and CD81 + exosomes carrying leukotrienes biosynthesis enzymes [102] BALF ↑Mitochondria/mitochondrial DNA in exosomes [103] BALF ↓Phosphatidylglycerol, ceramide-phosphates, and ceramides [104] ↑Sphingomyelin in exosomes…”

Section: Role Of Evs In the Pathogenesis Of Lung Diseasesmentioning

confidence: 99%

“…Rose et al showed that endothelial cell-derived MPs can act as biomarkers for right ventricular function in PAH patients, wherein the number of these particles in urine was found to be increased in PAH patients compared to healthy controls [97] (Table 1). A correlation between these urine MP biomarkers and tricuspid annular plane systolic excursion (TAPSE) was observed in PAH patients [97].…”

Section: Pulmonary Hypertension (Ph)mentioning

confidence: 99%

Extracellular vesicles: novel communicators in lung diseases

et al. 2020

View full text Add to dashboard Cite

The lung is the organ with the highest vascular density in the human body. It is therefore perceivable that the endothelium of the lung contributes significantly to the circulation of extracellular vesicles (EVs), which include exosomes, microvesicles, and apoptotic bodies. In addition to the endothelium, EVs may arise from alveolar macrophages, fibroblasts and epithelial cells. Because EVs harbor cargo molecules, such as miRNA, mRNA, and proteins, these intercellular communicators provide important insight into the health and disease condition of donor cells and may serve as useful biomarkers of lung disease processes. This comprehensive review focuses on what is currently known about the role of EVs as markers and mediators of lung pathologies including COPD, pulmonary hypertension, asthma, lung cancer and ALI/ARDS. We also explore the role EVs can potentially serve as therapeutics for these lung diseases when released from healthy progenitor cells, such as mesenchymal stem cells. Extracellular vesicles Cell-to-cell communication is essential for nearly all physiologic and metabolic processes. This intercellular conveyance is achieved through receptor ligands, signaling molecules, hormones, and extracellular vesicles (EVs). Historically, secretion of the cell in the form of EVs was considered as unimportant waste material, cellular "garbage bags," or dust particles [1-5]. However, in recent years, this so-called "waste" is now known to be of profound importance in various biological systems, creating a boon in their exploration across the scientific community. Lipid bilayer membrane-enclosed vesicles are secreted by both prokaryotic and eukaryotic cells [4-9]. Although, the term "extracellular vesicle" is sometimes used in reference to exosomes, it is actually a very broad term that encompasses all different types of vesicles secreted outside the cells [10]. Regardless, the function of all these vesicles appears to be all the same: communication between the cells within an organism or between species [11, 12]. In addition, it is not necessary that all vesicles secreted from cells are functional or have any role in some kind of biological process. Sometimes, they just act as "dustcart" to remove the waste from cells [13]. Of note, there have been discrepancies in the classification of these vesicles in the literature. Some studies divide EVs into two major categories: I) exosomes, defined as vesicles released by exocytosis of the multivesicular bodies; and II) ectosomes, defined as the vesicles which are assembled and released by the plasma membrane [14]. However, most recent studies categorize EVs as either exosome, microvesicles, microparticles, or apoptotic bodies based on vesicle size and how they are formed [15-21] (Fig. 1). Exosomes Exosomes are small EVs with sizes ranging between 30 and 150 nm in diameter that originate from the internal vesicles of multivesicular bodies (MVB) of nearly all cell types. Exosomes originating from different cell types have different composition; however, there are certain

show abstract

“…The level of CD105 + EMVs is related to severity, EC survival and angiogenesis in PH patients . The level of CD144 + EMVs is related to hemodynamic severity, pulmonary artery intima media thickness and right ventricular function . The release of CD144 + EMVs may reflect the structural destruction of the endothelium rather than an inflamed lung.…”

Section: Endothelial Microvesiclesmentioning

confidence: 99%

Endothelial microvesicles in hypoxic hypoxia diseases

Deng

Wang

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Hypoxic hypoxia, including abnormally low partial pressure of inhaled oxygen, external respiratory dysfunction‐induced respiratory hypoxia and venous blood flow into the arterial blood, is characterized by decreased arterial oxygen partial pressure, resulting in tissue oxygen deficiency. The specific characteristics include reduced arterial oxygen partial pressure and oxygen content. Hypoxic hypoxia diseases (HHDs) have attracted increased attention due to their high morbidity and mortality and mounting evidence showing that hypoxia‐induced oxidative stress, coagulation, inflammation and angiogenesis play extremely important roles in the physiological and pathological processes of HHDs‐related vascular endothelial injury. Interestingly, endothelial microvesicles (EMVs), which can be induced by hypoxia, hypoxia‐induced oxidative stress, coagulation and inflammation in HHDs, have emerged as key mediators of intercellular communication and cellular functions. EMVs shed from activated or apoptotic endothelial cells (ECs) reflect the degree of ECs damage, and elevated EMVs levels are present in several HHDs, including obstructive sleep apnoea syndrome and chronic obstructive pulmonary disease. Furthermore, EMVs have procoagulant, proinflammatory and angiogenic functions that affect the pathological processes of HHDs. This review summarizes the emerging roles of EMVs in the diagnosis, staging, treatment and clinical prognosis of HHDs.

show abstract

Flow Cytometric Quantification of Peripheral Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived Microparticles in Pulmonary Arterial Hypertension

Cited by 20 publications

References 44 publications

Pulmonary arterial hypertension treatment with carvedilol for heart failure: a randomized controlled trial

Pulmonary arterial hypertension treatment with carvedilol for heart failure: a randomized controlled trial

Extracellular vesicles: novel communicators in lung diseases

Endothelial microvesicles in hypoxic hypoxia diseases

Contact Info

Product

Resources

About