Flow cytometric investigation of neutrophil oxidative burst and apoptosis in physiological and pathological situations

Elbim, Carole; Lizard, Gérard

doi:10.1002/cyto.a.20726

Cited by 62 publications

(60 citation statements)

References 68 publications

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“…Not only did this patient have the most extensive disease burden, but the highest ROS Phenotypic Probes of Liposomal Pharmacology measurement. These results may indicate an environment of oxidative stress and imbalanced redox systems (Elbim and Lizard, 2009). …”

Section: Discussionmentioning

confidence: 98%

Translational Studies of Phenotypic Probes for the Mononuclear Phagocyte System and Liposomal Pharmacology

Caron

Lay

Fong

et al. 2013

J Pharmacol Exp Ther

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As nanoparticles (NPs) are cleared via phagocytes of the mononuclear phagocyte system (MPS), we hypothesized that the function of circulating monocytes and dendritic cells (MO/DC) in blood can predict NP clearance (CL). We measured MO/DC phagocytosis and reactive oxygen species (ROS) production in mice, rats, dogs, and patients with refractory solid tumors. Pharmacokinetic studies of polyethylene glycol (PEG)-encapsulated liposomal doxorubicin (PEGylated liposomal doxirubicin [PLD]), CKD-602 (S-CKD602), and cisplatin (SPI-077) were performed at the maximum tolerated dose. MO/DC function was also evaluated in patients with recurrent epithelial ovarian cancer (EOC) administered PLD. Across species, a positive association was observed between cell function and CL of PEGylated liposomes. In patients with EOC, associations were observed between PLD CL and phagocytosis (R 2 5 0.43, P 5 0.04) and ROS production (R 2 5 0.61, P 5 0.008) in blood MO/DC. These findings suggest that probes of MPS function may help predict PEGylated liposome CL across species and PLD CL in patients with EOC.

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Section: Discussionmentioning

confidence: 98%

Translational Studies of Phenotypic Probes for the Mononuclear Phagocyte System and Liposomal Pharmacology

Caron

Lay

Fong

et al. 2013

J Pharmacol Exp Ther

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“…The detection of caspase-3 has been the traditional method used to measure apoptosis. Due to the increase in commercially available fluorescent kits and novel instrumentations in the recent years, the detection of phosphatidylserine using annexin V has become more routinely performed [1,[3][4][5].…”

Section: Introductionmentioning

confidence: 99%

A rapid detection method for apoptosis and necrosis measurement using the Cellometer imaging cytometry

Chan¹,

Lai²,

Wang

et al. 2011

Apoptosis

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Apoptosis and necrosis play an important role in various aspects of preclinical pharmaceutical drug discovery and validation. The ability to quickly determine the cytotoxic effect of chemical compounds on cancer cells allows researchers to efficiently identify potential drug candidates for further development in the pharmaceutical discovery pipeline. Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis. Currently, fluorescence-based cell death assays have not been demonstrated by the Cellometer system, which can potentially provide a quick, simple, and inexpensive alternative method for smaller biomedical research laboratories. In this study, we demonstrate for the first time the use of Cellometer imaging cytometry for necrosis/apoptosis detection by studying the dose-response effect of heat and drug-induced cell death in Jurkat cells labeled with annexin V-FITC (apoptotic) and propidium iodide (necrotic). The experimental results were evaluated to validate the imaging cytometric capabilities of the Cellometer system as compared to the conventional flow cytometry. Similar cell population results were obtained from the two methods. The ability of Cellometer to rapidly and cost-effectively perform fluorescent cell-based assays has the potential of improving research efficiency, especially where a flow or laser scanning cytometer is not available or in situations where a rapid analysis of data is desired.

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“…FACS evaluates intracellular ROS. Of note is the fact that the dihydrorhodamine 123 (DHR) used to detect ROS binds to cellular and mitochondrial membranes, thus it detects an intracellular signal that responds to the presence of H 2 O 2 , specifically (Elbim, Lizard, 2009).…”

Section: Discussionmentioning

confidence: 99%

WBC count and functional changes induced by co-administration of clofazimine and clarithromycin, in single and multiple doses, in Wistar rats

Paina

Miranda

Tavares

et al. 2012

Braz. J. Pharm. Sci.

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Clofazimine and clarithromycin are used to treat leprosy and infections caused by Mycobacterium avium complex. Little data on the toxicity of co-administration of these two drugs are available. Here we evaluated the potential adverse effects of polytherapy with these two drugs in male Wistar rats by determining WBCs counts and other blood cell counts, neutrophilic phagocytosis, and burst oxidative, by flow cytometry. We observed an increase in WBCs, in multiple-dose regimens, and in polymorphonuclear cells, in both single-clarithromycin only and multiple dose regimens. We also observed a reduction in mononuclear cell counts in single and multiple doses. The drugs seem to reverse the mononuclear and polymorphonuclear cell ratio. An increase in oxidative burst was observed in animals treated with the drugs administered either individually or combined. In conclusion, clofazimine and clarithromycin change WBCs counts. Our results may contribute for a better understanding of the mechanisms related to the effects of co-administrating the two drugs.Uniterms: Clofazimine/adverse effects/experimental study. Clarithromycin/adverse effects/experimental study. Leprosy/treatment. Mycobacterium avium/infections/treatment. Leukocytes. Phagocytosis. Oxidative burst.Clofazimina e laritromicina são utilizadas no tratamento da hanseníase e em infecções causadas pelo complexo Mycobacterium avium. Devido à escassez de dados sobre a toxicidade de esquemas terapêuticos que associam estes fármacos, este estudo teve por objetivo avaliar os efeitos adversos desta terapia, em ratos machos Wistar, por meio da determinação da contagem global e específica de leucócitos e ensaios de fagocitose e burst oxidativo de neutrófilos por citometria de fluxo. Houve aumento do número de leucócitos (dose múltipla) e de células polimorfonucleares (doses única e múltipla) nos grupos tratados com claritromicina em monoterapia ou associada à clofazimina e redução das células mononucleares, em doses única e múltipla, nos mesmos grupos. Os fármacos parecem inverter a proporção entre células mono e polimorfonucleares. Observou-se aumento do burst oxidativo nos animais tratados com os fármacos isolados ou associados. Concluindo, clofazimina e claritromicina provocam alterações leucocitárias e os resultados podem contribuir para melhor entendimento dos mecanismos relacionados aos efeitos da administração dos fármacos em associação.Unitermos: Clofazimina/efeitos adversos/estudo experimental. Claritromicina/efeitos adversos/ estudo experimental. Hanseníase/tratamento. Mycobacterium avium/infecções/tratamento. Leucócitos. Fagocitose. Burst oxidativo.

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Flow cytometric investigation of neutrophil oxidative burst and apoptosis in physiological and pathological situations

Cited by 62 publications

References 68 publications

Translational Studies of Phenotypic Probes for the Mononuclear Phagocyte System and Liposomal Pharmacology

Translational Studies of Phenotypic Probes for the Mononuclear Phagocyte System and Liposomal Pharmacology

A rapid detection method for apoptosis and necrosis measurement using the Cellometer imaging cytometry

WBC count and functional changes induced by co-administration of clofazimine and clarithromycin, in single and multiple doses, in Wistar rats

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