Flow cytometric identification of sexually derived polyploids in hop (<i>Humulus lupulus</i>L.) and their use in hop breeding

Beatson, R. A.; Ferguson, A. R.; Weir, Iona E.; Graham, L. T.; Ansell, K. A.; Ding, Hao

doi:10.1023/b:euph.0000003882.23615.c5

Cited by 22 publications

(18 citation statements)

References 7 publications

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“…For each sample, runs were replicated on different days. The samples of the target species and reference plant were co-chopped with a double-sided razor blade in a precooled Petri dish containing 200 ll woody plant buffer (WPB, Loureiro et al, 2007b) with 3% PVP-10 (WPB2) as per Beatson et al (2003). From these preparations, nuclei were isolated and stained using the Cystain® PI Absolute P Kit (Partec, Munster, Germany) with the following modification: the kit extraction buffer was replaced with WPB2.…”

Section: Genome Sizesmentioning

confidence: 99%

Environmental adaptation in stomatal size independent of the effects of genome size

et al. 2014

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Cell sizes are linked across multiple tissues, including stomata, and this variation is closely correlated with genome size. These associations raise the question of whether generic changes in cell size cause suboptimal changes in stomata, requiring subsequent evolution under selection for stomatal size. We tested the relationships among guard cell length, genome size and vegetation type using phylogenetically independent analyses on 67 species of the ecologically and structurally diverse family, Proteaceae. We also compared how genome and stomatal sizes varied at ancient (among genera) and more recent (within genus) levels. The observed 60-fold range in genome size in Proteaceae largely reflected the mean chromosome size. Compared with variation among genera, genome size varied much less within genera (< 6% of total variance) than stomatal size, implying evolution in stomatal size subsequent to changes in genome size. Open vegetation and closed forest had significantly different relationships between stomatal and genome sizes. Ancient changes in genome size clearly influenced stomatal size in Proteaceae, but adaptation to habitat strongly modified the genome–stomatal size relationship. Direct adaptation to the environment in stomatal size argues that new proxies for past concentrations of atmospheric CO2 that incorporate stomatal size are superior to older models based solely on stomatal frequency.

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Section: Genome Sizesmentioning

confidence: 99%

Environmental adaptation in stomatal size independent of the effects of genome size

et al. 2014

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“…This approach is used in breeding of hop (Beatson et al 2003) and may have the added advantage that it will provide a bridge to hybridisation with other acacia taxa, as has been observed in many plant species (Husband 2004).…”

Section: X Breedingmentioning

confidence: 99%

Breeding polyploid varieties of tropical acacias: progress and prospects

Griffin

Quynh

Harbard

et al. 2015

Southern Forests: a Journal of Forest Science

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The paper reviews a decade of progress with breeding new polyploid varieties of Acacia mangium and other tropical acacias in Vietnam, and outlines plans for future strategies to complement conventional diploid breeding. Polyploid breeding is a means of introducing diversity into breeding populations, of making step-change improvements in wood fibre and other properties, and reducing reproductive output of these potentially weedy taxa. To date we have demonstrated that A. mangium tetraploids have larger and thicker wood fibres and we have strong preliminary evidence that the reproductive output of triploids is indeed reduced. Other potential benefits such as wind and stress tolerance and disease resistance are under field evaluation. Trials demonstrate that colchicine-induced tetraploids, whether derived from pure A. mangium seeds or from commercial hybrid clones, are substantially slower growing than diploids. While not the sole determinant of crop value, volume production is hugely important to growers, so long-term success of the program is critically dependent upon demonstration that this problem can be resolved in advanced generations. Future emphasis will be on production of triploid and tetraploids from the interspecific hybrid A. mangium  A. auriculiformis. The better rooting ability contributed by A. auriculiformis maximises prospects for operational cloning of select trees. Highly heterozygous hybrids also offer the better prospect for exploiting heterosis. A strategy for developing a diverse breeding population of neo-tetraploid F 1 hybrids is presented.

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“…However, seedless hops typically produce more essential oil ( Briggs et al ., 2004 ). An alternative method is the production of seedless triploid varieties, which have three sets of chromosomes instead of the normal two (diploid), and produce normal harvest yields ( Beatson et al ., 2003 ).…”

Section: Variation and Changes In Compositionmentioning

confidence: 99%

“…Whole hops are the most susceptible to oxidation during storage, with signifi cant losses of resins and essential oil occurring. Between 50% and 70% of the essential oil can be lost during 6 months storage at 20°C, mainly due to loss of myrcene ( Beatson et al ., 2003 ). Therefore, hop bales should ideally be stored at refrigeration temperatures (0-5°C) ( Forster, 2001 ).…”

Section: Variation and Changes In Compositionmentioning

confidence: 99%