Flow cytometric DNA ploidy, p53, PCNA, and c-erbB-2 protein expressions as predictors of survival in surgically resected gastric cancer patients

Danesi, Donatella Tirindelli; Spanò, Marcello; Fabiano, Alfredo; Altavista, Pierluigi; Pasqualetti, Patrizio; Toscano, Maria Gabriella; Antonini, Fabiana; Catalano, Piera; Mecozzi, Antonella; Picconi, A; Daffinà, Antonio; Cucchiara, G.

doi:10.1002/(sici)1097-0320(20000215)42:1<27::aid-cyto5>3.0.co;2-n

Cited by 21 publications

(19 citation statements)

References 41 publications

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“…The samples with nuclear p53 only (and negative for nuclear b-catenin) also showed higher ABI of 60% (Figure 4g; Po0.001) than those negative for both. These results are consistent with the report (Danesi et al, 2000) showing association between p53 overexpression and aneuploidy, and suggest the validity of our ABI analysis. They also indicate that b-catenin/TCF transcriptional activation contributes to CIN in the intestinal-type gastric cancer too, independent of the p53 status.…”

Section: Es Cells With High Abi Produce New Chromosomal Aberrationssupporting

confidence: 93%

“…In the intestinal-type gastric cancer, samples with nuclear b-catenin localization had higher ABI, with a stronger statistical significance (Figure 4e; P ¼ 0.014), whereas in the diffuse type, there was less correlation (Figure 4f; P ¼ 0.14). As nuclear expression of p53 is implicated in CIN in gastric cancer (Danesi et al, 2000), we also examined p53 (Figure 4d), and its relationship with ABI. For the intestinal-type gastric cancer, the samples negative for nuclear expression of both b-catenin and p53 showed ABI of 33% (Figure 4g).…”

Section: Es Cells With High Abi Produce New Chromosomal Aberrationsmentioning

confidence: 99%

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Chromosomal instability by β-catenin/TCF transcription in APC or β-catenin mutant cells

Aoki

Sugai

et al. 2006

Oncogene

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Adenomatous polyposis coli (APC/Apc) gene encodes a key tumor suppressor whose mutations activate b-catenin/ T-cell factor (TCF)-mediated transcription (canonical Wnt signaling). Here, we show that Wnt signaling can cause chromosomal instability (CIN). As an indicator of CIN, we scored anaphase bridge index (ABI) in mouse polyps and ES cells where Wnt signaling was activated by Apc or b-catenin mutations. We found three to nine times higher ABI than in wild-type controls. Furthermore, karyotype analysis confirmed that the Wnt signalactivated ES cells produced new chromosomal aberrations at higher rates; hence CIN. Consistently, expression of dominant-negative TCFs in these cells reduced their ABI. We also found that Wnt signal activation increased phosphorylation of Cdc2 (Cdk1) that inhibited its activity, and suppressed apoptosis upon exposure of the cells to nocodazole or colcemid. The data suggest that Wnt signaling stimulates the cells to escape from mitotic arrest and apoptosis, resulting in CIN. In human gastric cancer tissues with nuclear b-catenin, ABI was significantly higher than in those without. These results collectively indicate that b-catenin/TCF-mediated transcription itself increases CIN through dysregulation of G2/M progression.

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Section: Es Cells With High Abi Produce New Chromosomal Aberrationssupporting

confidence: 93%

Section: Es Cells With High Abi Produce New Chromosomal Aberrationsmentioning

confidence: 99%

Chromosomal instability by β-catenin/TCF transcription in APC or β-catenin mutant cells

Aoki

Sugai

et al. 2006

Oncogene

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“…When all patients were pooled they did not observe any clinical relevance [12]. In addition, Danesi et al suggested that DNA ploidy may act as a prognostic factor only in early-stage tumours (stages I and II) [13].…”

Section: Aneuploidy and Gastric Cancermentioning

confidence: 99%

Clinical impact of aneuploidy on gastric cancer patients

2009

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Gastric cancer is a leading cause of death worldwide. Nowadays, complete surgical resection and TNM at diagnosis are the main prognostic factors. In spite of this, many patients will have a recurrence after surgery and die within a few months or years. That means that we need more accurate prognostic factors to design specific approaches for individual patients. Chromosome instability is a feature of gastric cancer commonly associated to chromosomal aberrations that leads to major modifications of DNA content globally termed as aneuploidy. In this regard, many authors' opinions diverge regarding the clinical impact of aneuploidy. This review will summarise data on the clinical impact of aneuploidy on clinical practice, the biological mechanisms that underlie chromosomal instability that induces aneuploidy and the relevance of specific chromosomal aneuploidy to cancer biology.

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“…Bu araş tır ma la rın ter si ne hiç bir kli ni ko pa to lo jik pa ra met re ile p53 ara sın da iliş ki sap ta ma yan ça-lış ma lar da var dır. [31][32][33][34] Sağ ka lım açı sın dan ya pı lan ça lış ma lar da Le e ve ark. 34 sa de ce dif füz tip kar sinom da, Ro vi el lo ve ark.…”

Section: Re Si̇munclassified

The Correlation of C-Erbb-2 (Her-2/Neu) and p53 Expressions with Prognostic Parameters in Patients with Gastric Carcinoma

Ton¹,

Başaran²,

Kabukçuoğlu³

2011

Turkiye Klinikleri J Med Sci

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ide kanserleri, görülme sıklığı son yıllarda azalmakla birlikte akciğer kanserinden sonra kansere bağlı ölümlerin en sık görüldü-ğü ikinci kanser türü olmaya devam etmektedir.1 Bu açıdan mide karsinomunun erken saptanması ve önlenmesi için yoğun çabalar sarf edilmektedir. Gastrektomi ile birlikte genişletilmiş lenfadenektomi, cerrahi ile Turkiye Klinikleri J Med Sci 2011;31(3) 575Mide Karsinomlarında C-Erbb-2 (Her-2/Neu) ve P53 Ekspresyonlarının Prognostik Parametrelerle İlişkisi Ö ÖZ ZE ET T A Am ma aç ç: : Spe si fik ola rak c-erbB-2 (HER-2/ne u)'yi he def le yen in san mo nok lo nal an ti ko ru Hercep tin, c-erbB-2'yi aşı rı eks pre se eden tü mör le re kar şı in hi bi tör ak ti vi te ser gi ler ve c-erbB-2 po zitif me me kan ser li va ka lar da sağ ka lı ma fay da sağ la ma sı ne de niy le te ra pö tik kul la nım için uy gun bu lun muş tur. Son za man lar da c-erbB-2'nin prog nos tik be lir le yi ci li ği ve gas trik ade no kar si nom larda te da vi nin he de fi ol ma sı ko nu sun da ça lış ma lar ya pıl mak ta dır. Bu ça lış ma nın ama cı mi de kar sinom la rın da c-erbB-2 ve p53 pro te in eks pres yon la rı nın ve prog nos tik pa ra met re ler le iliş ki si nin araş tı rıl ma sı dır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Elli dört gas trik kar si nom va ka sın da c-erbB-2 ve p53 eks presyon la rı im mü no his to kim ya sal yön tem le in ce len di ve prog nos tik pa ra met re ler le iliş ki si araş tı rıl dı. B Bu ul l g gu u l la ar r: : c-erbB-2 ve p53 eks pres yon oran la rı sı ra sıy la %11.1 (6/54) ve %74.1 (40/54) ola rak saptan dı. c-erbB-2 po zi tif tü mör le rin ta ma mı La u ren kla si fi kas yo nu na gö re in tes ti nal tip tey di. c-erbB-2, p53 ve prog nos tik pa ra met re ler ara sın da is ta tis tik sel ba kım dan an lam lı bir iliş ki sap tan ma dı. S So o n nu uç ç: : c-erbB-2 ve p53 pro te in le ri nin mi de kar si nom la rın da be lir li se vi ye ler de eks pre se ol du ğu, ancak prog nos tik pa ra met re ler le ara sın da her han gi bir iliş ki ol ma dı ğı so nu cu na va rıl mış tır.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Mide tümörleri; erbb2 proteini, insan; tümör süpresor proteini p53A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : The mo noc lo nal hu man an ti body Her cep tin, which spe ci fi cally tar gets cerbB-2 (HER-2/ne u), ex hi bits growth-in hi bi tory ac ti vity aga inst c-erbB-2 ove rex pres sing tu mors and is fo und su i tab le for the ra pe u tic use du e to pro ved sur vi val be ne fit in pa ti ents with c-erbB-2-po si ti ve bre ast can cer. Re cently, so me stu di es investigated c-erbB-2 both as a prog nos tic de no mina tor and as a tar get of tre at ment in gas tric ade no car ci no mas. The aim of this study was to in vesti ga te c-erbB-2 and p53 pro te in ex pres si ons and the ir re la ti on to the prog nos tic pa ra me ters in gas tric car ci no mas. Cur rent study aims to in ves ti ga te the cor re la ti on of c-erbB-2 and p53 pro te in ex pressi ons with prog nos tic pa ra me ters.M Ma a t te e r ri i a al l a an...

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Flow cytometric DNA ploidy, p53, PCNA, and c-erbB-2 protein expressions as predictors of survival in surgically resected gastric cancer patients

Cited by 21 publications

References 41 publications

Chromosomal instability by β-catenin/TCF transcription in APC or β-catenin mutant cells

Chromosomal instability by β-catenin/TCF transcription in APC or β-catenin mutant cells

Clinical impact of aneuploidy on gastric cancer patients

The Correlation of C-Erbb-2 (Her-2/Neu) and p53 Expressions with Prognostic Parameters in Patients with Gastric Carcinoma

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