Flow cytometric DNA analysis of cirrhotic liver cells in patients with hepatocellular carcinoma can provide a new prognostic factor

Ruá, Savino; Comino, Alberto; Fruttero, A; Torchio, Pierfederico; Bouzari, Hedayat; Taraglio, Stefano; Torchio, Bruno; Capussotti, Lorenzo

doi:10.1002/(sici)1097-0142(19960915)78:6<1195::aid-cncr5>3.0.co;2-9

Cited by 30 publications

(6 citation statements)

References 27 publications

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“…Moreover, active cirrhosis was significantly (P<0.05) associated with a higher PI (S + G2M phases) than diploid cases with inactive cirrhosis and my due to liver response to cell injury (Pahlavan et al, 2006;Schaffner 1991). The value of detecting these cellular DNA changes in liver cirrhosis by FCM is of prognostic value in regards to early detection of preneoplastic changes in liver cirrhosis were found to be at increased risk of developing HCC (Clemente et al, 2007;Ruà et al, 1996;Tarao et al, 1994b). The presence of an aneuploid DNA pattern had been reported by Hoso and Nakanuma (1991), to a precancerous change in cases with atypical adenomatous hyperplasia, of the liver.…”

Section: Dna Ploidy and Liver Cell Dysplasia In Liver Biopsies From Psupporting

confidence: 89%

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Tabll¹,

Ismail²

2011

Liver Biopsy

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Section: Dna Ploidy and Liver Cell Dysplasia In Liver Biopsies From Psupporting

confidence: 89%

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Tabll¹,

Ismail²

2011

Liver Biopsy

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“…Polyploid hepatocytes isolated within 5 days of partial hepatectomy are less proliferative than diploid hepatocytes ( Gorla et al, 2001 ), suggesting that partial hepatectomy-induced polyploid hampers cell proliferation. The notion that diploid cells proliferate faster than polyploid hepatocytes is also supported by several studies which observed that diploid hepatocytes are enriched in patients and rats with various disease states ( Anti et al, 1994 ; Rua et al, 1996 ; Gandillet et al, 2003 ).…”

Section: Regenerationmentioning

confidence: 80%

Cellular polyploidy in organ homeostasis and regeneration

et al. 2022

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Polyploid cells, which contain more than one set of chromosome pairs, are very common in nature. Polyploidy can provide cells with several potential benefits over their diploid counterparts, including an increase in cell size, contributing to organ growth and tissue homeostasis and improving cellular robustness via increased tolerance to genomic stress and apoptotic signals. Here, we focus on why polyploidy in the cell occurs and which stress responses and molecular signals trigger cells to become polyploid. Moreover, we discuss its crucial roles in cell growth and tissue regeneration in the heart, liver and other tissues.

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“…The prognostic importance of the mitotic index and tumor stage is appreciated in a number of malignancies ( 52 , 53 ). In addition, its value is similar to that of techniques such as nuclear antigen of cell proliferation ( 54 ) or DNA analysis ( 55 , 56 ). In the context of HCC, there is evidence that a high mitotic index is an unfavorable prognostic indicator ( 24 , 25 , 57 , 58 ), as well as an independent predictor of short-term survival in patients who undergo hepatectomy with curative intent ( 23 ).…”

Section: Discussionmentioning

confidence: 96%

Inositol 1,4,5‑trisphosphate receptor type 3 is involved in resistance to apoptosis and maintenance of human hepatocellular carcinoma

et al. 2021

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The expression of the inositol 1,4,5-trisphosphate receptor type 3 (ITRP3) in hepatocytes is a common event in the pathogenesis of hepatocellular carcinoma (HCC), regardless of the type of underlying liver disease. However, it is not known whether ITPR3 expression in hepatocytes is involved in tumor maintenance. The aim of the present study was to determine whether there is an association between ITPR3 expression and clinical and morphological parameters using HCC samples obtained from liver explants from patients (n=53) with different etiologies of underlying chronic liver disease (CLD). ITPR3 expression, mitosis and apoptosis were analyzed in human liver samples by immunohistochemistry. Clinical and event-free survival data were combined to assess the relationship between ITPR3 and liver cancer growth in patients. RNA sequencing analysis was performed to identify apoptotic genes altered by ITPR3 expression in a liver tumor cell line. ITPR3 was highly expressed in HCC tumor cells relative to adjacent CLD tissue and healthy livers. There was an inverse correlation between ITPR3 expression and mitotic and apoptotic indices in HCC, suggesting that ITPR3 contributed to the maintenance of HCC by promoting resistance to apoptosis. This was confirmed by the upregulation of CTSB, CHOP and GADD45, genes involved in the apoptotic pathway in HCC. The expression of ITPR3 in the liver may be a promising prognostic marker of HCC

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Flow cytometric DNA analysis of cirrhotic liver cells in patients with hepatocellular carcinoma can provide a new prognostic factor

Abstract: The results of this study suggest that S-phase fraction of cirrhotic liver parenchyma may be employed as a new parameter in the prognostic evaluation of HCC patients.

Cited by 30 publications

References 27 publications

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Cellular polyploidy in organ homeostasis and regeneration

Inositol 1,4,5‑trisphosphate receptor type 3 is involved in resistance to apoptosis and maintenance of human hepatocellular carcinoma

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