Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1

Lucidi, Cynthia de A.; Takahira, Regina Kiomi; Gerlach, John; Davis, John M.; Schwartz, Kenneth A.; Scott, Michael A.

doi:10.1111/j.1939-165x.2011.00374.x

Cited by 8 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…a,5, [14][15][16] Recently, flow cytometry with the anti-DEA 1.X polyclonal antibody was used experimentally to type erythrocytes for DEA 1.1, but DEA 1.1 and 1.2 expression levels or their variation among dogs were not examined. 17 Originally, DEA 1.1 typing was done with a polyclonal DEA 1.1 antiserum derived by alloimmunizing dogs with different blood types; this reagent was a weak agglutinin and required canine antiglobulin (Coombs') reagent to better visualize the agglutination reaction in the tube or microtiter assay. 5,6,10,13 Two murine monoclonal anti-DEA 1.1 antibodies, introduced in the 1990s, are used in typing cards, b gel columns, c and immunochromatographic strips.…”

mentioning

confidence: 99%

“…5,6,10,13 Two murine monoclonal anti-DEA 1.1 antibodies, introduced in the 1990s, are used in typing cards, b gel columns, c and immunochromatographic strips. d, 13,[17][18][19][20][21] It has been suspected that DEA 1.2+ blood gives a weakly positive DEA 1.1 result. 13 Because these monoclonal antibodies were never properly evaluated against dogs which tested DEA 1.1+ or DEA 1.2+, we will refer to them as anti-DEA 1 rather than anti-DEA 1.1 antibodies.…”

mentioning

confidence: 99%

See 1 more Smart Citation

DEA1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques

Acierno

Raj

Giger

2014

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundThe Dog erythrocyte antigen (DEA) 1 blood group system was thought to contain types DEA 1.1 and 1.2 (and possibly 1.3 [A3]). However, DEA 1.2+ dogs are very rare and newer typing methods reveal varying degrees of DEA 1 positivity.ObjectivesTo assess if variation in DEA 1 positivity is because of quantitative differences in surface antigen expression. To determine expression patterns in dogs over time and effects of blood storage (4°C). To evaluate DEA 1.2+ samples by DEA 1 typing methods.AnimalsAnticoagulated blood samples from 66 dogs in a research colony and from a hospital, and 9 previously typed DEA 1.2+ dogs from an animal blood bank.MethodsResearch study: Samples were analyzed by flow cytometry and immunochromatographic strip using a monoclonal anti‐DEA 1 antibody.ResultsTwenty dogs were DEA 1−, whereas 46 dogs were weakly to strongly DEA 1+. Antigen quantification revealed excellent correlation between strip and flow cytometry (r = 0.929). Both methods reclassified DEA 1.2+ samples as weakly to moderately DEA 1+, but they were not retyped with the polyclonal anti‐DEA 1.1/1.X antibodies. Dogs and blood samples retained their relative DEA 1 antigen densities over time.Conclusions and Clinical ImportanceThe blood group system DEA 1 is a continuum from negative to strongly positive antigen expression. Previously typed DEA 1.2+ appears to be DEA 1+. These findings further the understanding of the DEA 1 system and suggest that all alleles within the DEA 1 system have a similarly based epitope recognized by the monoclonal antibody.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

DEA1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques

Acierno

Raj

Giger

2014

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…In the dog studies by Slichter et al, 15 donors and recipients expressed the dog erythrocyte antigen 1.1 (Oral communication, Dr. Sherrill Slichter, January 2019). But this model has limits since dog platelets do not express dog erythrocyte antigen 1.1 . Finally, it must be noted that healthy, animal facility–housed, sterile chow–fed, experimental animals likely have a different immune response compared to patients receiving immunosuppressive treatment for hematologic cancers …”

mentioning

confidence: 99%

“…But this model has limits since dog platelets do not express dog erythrocyte antigen 1.1. 20 Finally, it must be noted that healthy, animal facility-housed, sterile chow-fed, experimental animals likely have a different immune response compared to patients receiving immunosuppressive treatment for hematologic cancers. [21][22][23] Fortunately, we may receive more data soon to help us find answers: The recently published EFFIPAP trial 24 collected data on HLA alloimmunization, and these are currently undergoing analysis.…”

mentioning

confidence: 99%

Pathogen reduction and HLA alloimmunization: more questions than answers

Stolla

2019

Transfusion

View full text Add to dashboard Cite

“…This method has also been used in veterinary hematology to detect DEA 1 from antibodies using this methodology. Lucidi et al (2011) researched DEA 1 in platelets but did not identify it while Polak et al (2015) used the technique to research DEA 1 in erythrocytes using this methodology.…”

mentioning

confidence: 99%

Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion

Santos

Moroz

Santos

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

The shortage of blood donor dogs in veterinary emergencies can lead to blood transfusions between animals whose blood type has not been identified. The antibody profile serves as a warning sign for animals that require a second blood transfusion, which is only advisable from compatible donor dogs. This article focuses on the determination of anti-DEA 1 antibodies using the flow cytometry technique in dogs that have undergone a transfusion using DEA 1-positive blood, compared to results obtained from crossmatching. Blood from 18 DEA 1-positive donors ranked according to the chromatographic technique was used to transfuse thirty-three animals with unknown blood types and which demonstrated negative crossmatching to donors. On post-transfusion days 7, 14, 21 and 28, 45% and 27% of the animals tested positive for the anti-DEA 1 antibody, through crossmatching and flow cytometry, respectively. Detecting antibodies using the flow cytometric technique has high specificity and sensitivity, while crossmatching methods are highly sensitive but manifest low specificity. Following the blood transfusion, animals that did not present as positive through crossmatching or flow cytometry were considered different from all other DEA 1-positive blood groups.A escassez de cães doadores de sangue em situações de emergência na Medicina Veterinária pode levar à realização de transfusões de sangue entre animais que não tiveram seu tipo sanguíneo previamente determinado. O padrão de anticorpos serve como um sinal de alerta para animais que serão submetidos a uma segunda transfusão sanguínea, sendo esta recomendável somente se o sangue for proveniente de cães doadores compatíveis. Este artigo aborda a determinação de anticorpos anti-AEC 1 pela técnica de citometria de fluxo em cães que receberam uma transfusão utilizando sangue do grupo AEC 1 positivo, comparando-se os resultados com aqueles obtidos a partir de reação cruzada. Foi utilizado o sangue de 18 animais doadores do tipo AEC 1 positivo classificados por técnica cromatográfica, a fim de transfundir trinta e três animais com tipos sanguíneos desconhecidos e que demonstraram reação cruzada negativa aos doadores. Nos dias 7, 14, 21 e 28 pós-transfusão, 45% e 27% dos animais mostraramse positivos para os anticorpos anti-AEC 1, respectivamente, pela reação cruzada e através de citometria de fluxo. A pesquisa de anticorpos com o emprego da técnica de citometria de fluxo tem alta especificidade e sensibilidade, enquanto a reação cruzada, altamente sensível, tem baixa especificidade. Animais que não apresentaram positividade após a transfusão de sangue na reação cruzada e na citometria de fluxo foram considerados como pertencentes a grupos sanguíneos diferentes do AEC 1 positivo. Palavras-chave: Isoanticorpos. Anticorpos caninos. AEC 1. Transfusão sanguínea em cães.

show abstract

Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1

Cited by 8 publications

References 31 publications

DEA1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques

DEA1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques

Pathogen reduction and HLA alloimmunization: more questions than answers

Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion

Contact Info

Product

Resources

About