Flow Cytometric Analysis of T cell Subset in Bursa of Fabricius in Broiler Chicken (Gallus domesticus)

Kempashi, Jayachandra; Kannan, T.; Basha, Sabiha Hayath; Raja, Aarti; Ramesh, Geetha

doi:10.20546/ijcmas.2017.602.171

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…CD3+ is the prominent T cell produces the antibody clone with high reactivity (Nagel et al, 2014). CD4+ and CD8+ potentially play as cell-mediated immunity during the antibody synthesis and support to destruct the infected cells (Kempashi et al, 2017). The shrinkage of T cells subset in the lymphoid tissue dramatically changes the circulatory phenomenon not only in the haematological aspects but also the antibody synthesis (Yang et al, 2018).…”

Section: Dmentioning

confidence: 99%

Recent Update: Effects of Aflatoxin in Broiler Chickens

Kurniasih¹,

Prakoso²

2019

jwpr

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Aflatoxin is a worldwide problem in poultry industries as it is known to contaminate poultry feed. Aflatoxin induces stress and increases mortality rate during infection in poultry, especially broiler chickens. The objectives of this study was to observe the pathological effects due to aflatoxicosis in broiler chickens. A total of 120 chickens were divided into four groups, group A fed with a basal diet without aflatoxin contamination, group B with aflatoxin (> 1 ppb < 50 ppb), group C with aflatoxin (> 51 ppb < 100 ppb), group D with aflatoxin (> 101 ppb < 150 ppb). The data were collected and analysed on day 7, 14, and 21. The results showed that diet with high aflatoxin contamination in group D impaired the physical and laboratory performances of the chickens, increased the risk of residue in the poultry's final products. The varying doses of aflatoxin contamination in the chicken feed causes the problems on the broiler chickens with different level of severity.

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Section: Dmentioning

confidence: 99%

Recent Update: Effects of Aflatoxin in Broiler Chickens

Kurniasih¹,

Prakoso²

2019

jwpr

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“…Poultry aflatoxicosis reduces humoral and cellular immune response and responds poorly to vaccination. Representation regarding CD4+ and CD8+ lymphocytes is used as the indicator of cell-mediated immunity (CMI) [ 56 ]. CD4+lymphocytes were the prominent lymphocytes that produce a signal to the B cells in the BF to promote antibody production [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

The Role ofSauropus androgynus(L.) Merr. Leaf Powder in the Broiler Chickens Fed a Diet Naturally Contaminated with Aflatoxin

Prakoso

Puspitasari

Rini

et al. 2018

Journal of Toxicology

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Aflatoxin (AF) is the secondary metabolite of Aspergillus flavus and commonly contaminates feed during storage. AF causes lowered growth rate, stress, and increased mortality in the poultry, especially for broiler industries. The aims of this study are to determine the effects of Sauropus androgynus (L.) Merr. leaf powder (SAP) in the chickens fed a diet naturally contaminated with AF. A total of 108 chickens are divided into 6 group: group I fed with basal diet (AF not detectable); group II fed with basal diet (AF not detectable) + 5% SAP; group III with AF (>1 ppb <50 ppb); group IV with AF (>1 ppb <50 ppb) + 5% SAP; group V with AF (>51 ppb <100 ppb) + 5% SAP; group VI with AF (>101 ppb <150 ppb) + 5% SAP. The data of the body weight, feed intake and efficiency, the relative weight of liver, kidney, spleen, bursa of Fabricius (BF), histopathology, haematological profile, haemagglutination inhibition (HI) titer, AF residue, and immunohistochemistry are collected on days 7, 14, and 21. All the data were analysed using SPSS 16. The supplementation of 5% SAP in the chickens fed a diet naturally contaminated with AF showed the potential effects of the body weight performance, haematological profile protection, increase in the cellular and humoral immune responses, reduction of AF residue in the organ, protection of liver, kidney, spleen, and BF histopathology, and increase in the immune-expression of CD4+/CD8+ lymphocytes ratio (P < 0.05). It shows that 5% SAP can be used as the alternative herbal supplementation to depress the impacts of aflatoxicosis in the broiler chickens.

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