Flow cytometric analysis of organ preservation-induced endothelial cell membrane damage

Ultrastructural Analysis of Human Endothelial Cells after Hypothermic Storage in Organ Preservation Solutions

“…Consistent with the results from in vivo investigations [2][3][4], University of Wisconsin (UW) solution was superior for endothelial preservation in several studies using cultured endothelial cells [5][6][7][8][9][10]. Human umbilical vein endothelial cell (HUVEC) cultures are well characterized [11,12] and represent, together with isolated endothelial cells of different sources, established models for preservation studies [5][6][7][8][9][10][13][14][15][16]. Although cultured cells have limitations due to the exclusion of interactions between different tissue compartments, they provide specific insights into stress responses and mechanisms on a cellular level.…”

“…Therefore, endothelial cells are of primary importance for the assessment of the efficiency of organ storage solutions [1]. Consistent with the results from in vivo investigations [2][3][4], University of Wisconsin (UW) solution was superior for endothelial preservation in several studies using cultured endothelial cells [5][6][7][8][9][10]. Human umbilical vein endothelial cell (HUVEC) cultures are well characterized [11,12] and represent, together with isolated endothelial cells of different sources, established models for preservation studies [5][6][7][8][9][10][13][14][15][16].…”

Dendrimer‐Functionalized Iron Oxide Nanoparticles for Specific Targeting and Imaging of Cancer Cells

“…FDA stains live cells, while PI stains dead cells. The stained cells were quantified by flow cytometry as described by Killinger [52]. Briefly, 6 × 10 4 KB cells per well were seeded into a 24-well plate and incubated with 0-150 lg mL Determination of Binding Affinity by Flow Cytometry: Approximately 1 × 10 5 cells per well were seeded in 24-well plates the day before the experiments.…”

Inflammatory cytokine regulation of TRAIL-mediated apoptosis in thyroid epithelial cells

“…Cell viability was determined by staining with fluorescein diacetate (FDA) and propidium iodide (PI) quantitated by flow cytometry as described by Killinger et al 55 PI positive and FDA negative cells were considered dead. Flow cytometric analysis of caspase-specific proteolytic cleavage of cytokeratin 18 (M30 CytoDEATH 1 monoclonal Ab) and Annexin V binding (Annexin V-FLUOS staining kit) were used as described by the manufacturer to confirm that apoptosis had occurred (both from Roche Molecular Biochemicals).…”