Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism

Li, Xican; Hu, Qian-Nan; Jiang, Shuxia; Li, Fēi; Lin, Jian; Han, Lu; Hong, Yaping; Lu, Wange; Gao, Yaoxiang

doi:10.1016/j.jscs.2014.06.004

Cited by 64 publications

(37 citation statements)

References 17 publications

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“…1) Our recent studies have demonstrated that a phenolic acid ester (chlorogenic acid) and a phenolic ketone (maclurin) could also effectively protect MSCs from damage induced by hydroxyl radical (·OH), which is the most harmful form of ROS. 9,10) As a special phytophenol, sinapine however contains an N atom in the molecule (Fig. 1).…”

Section: Protective Effect Of Sinapine Against Hydroxyl Radical-inducmentioning

confidence: 99%

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

Han

et al. 2016

CHEMICAL & PHARMACEUTICAL BULLETIN

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As a phenolic alkaloid occurring in Cruciferous plants, sinapine was observed to protect mesenchymal stem cells (MSCs) against ·OH-induced damage in this study. It was also found to prevent DNA from damage, to scavenge various free radicals (·OH, ·O 2 , 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt) (ABTS) · , and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·)), and to reduce Cu 2 to Cu . To further explore the mechanism, the end-product of sinapine reaction with DPPH· was determined using HPLC-electrospray ionization ( ·OH-induced oxidative damage is radical-scavenging, which is thought to be via hydrogen atom (H·) transfer (HAT) (or sequential electron (e) proton transfer (SEPT))→RAF pathways.Key words sinapine; mesenchymal stem cell; antioxidant mechanism; Cruciferous plant; ·OH-induced damage; hydrogen atom (H·) transfer (HAT) (or sequential electron (e) proton transfer (SEPT)) radical adduct formation (RAF) Recently, some phytophenols have been reported to suppress reactive oxygen species (ROS)-induced oxidative stress in mesenchymal stem cells (MSCs).1) MSCs are known as multipotent fibroblast cells that give rise to cells of the skeletal connective tissue including osteoblasts, chondrocytes, and adipocytes. However, their clinical applications are limited in their expandability and differentiation capacity, because oxidative stress from ROS impact a number of cellular processes, including cell adhesion, migration, and proliferation.2) Phytophenols refer to the phenolic compounds occurring in medicinal plants (especially Chinese herbal medicines). Phytophenols which can suppress ROS-induced oxidative stress, therefore, may suggest a possible new approach to maintain MSC viability and potency for clinical application.

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Section: Protective Effect Of Sinapine Against Hydroxyl Radical-inducmentioning

confidence: 99%

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

Han

et al. 2016

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…The protective effect of (+) taxifolin against •OH-induced bmMSC damage was investigated based on the method described in [ 16 , 17 ] with slight modifications. Briefly, bmMSCs were seeded at 5000 cells per well into 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells

Xie

Jiang

et al. 2017

Cell Mol Biol Lett

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The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+.Electronic supplementary materialThe online version of this article (10.1186/s11658-017-0066-9) contains supplementary material, which is available to authorized users.

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“…To study the protective effect of YP, DNA (0.15 mg/mL) was subjected to UV-induced hydroxyl radicals (·OH) in the presence or absence of YP (0.05, 0.11, and 0.17 mg/mL) using the method of Li et al [40], with modification. Hydrogen peroxide (H 2 O 2 ; 50 µL; 1.5 × 10 −2 mol·L −1 ) and DNA solution (100 µL) were mixed and irradiated with UV (130 Lux) in the presence or absence of YP for 2 min at room temperature.…”

Section: Protective Effect Against Dna Damagementioning

confidence: 99%

DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)

Zhao

Zhao²,

Ma³

et al. 2020

Molecules

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Yam yellow pigments (YP) are natural pigments formed during the storage of freshly cut yam (Dioscorea opposita) under certain conditions. The interaction of YP with calf thymus DNA (ctDNA) and its protective effect against DNA oxidative damage were investigated using multiple spectroscopic techniques, competitive binding experiments, viscosity measurements, and gel electrophoresis. Results showed that YP participated in intercalative binding with ctDNA. YP exhibited a protective effect against hydroxyl-induced DNA damage, which was attributed to the high hydroxyl radical scavenging activity of YP. Our findings improve our understanding of the mechanism of interaction between YP and ctDNA, and provide a theoretical basis for the application of YP in the food and drug industry.

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Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism

Cited by 64 publications

References 17 publications

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells

DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)

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