Flocculation performance and mechanism of fungal pellets on harvesting of microalgal biomass

Pei, Xuanyuan; Ren, Hongyan; Liu, Bingfeng

doi:10.1016/j.biortech.2020.124463

Cited by 59 publications

(15 citation statements)

References 45 publications

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“…circinelloides , achieving an efficiency of up to 99.7%. A similar result was found by Pei et al, 2021 [ 37 ] in a consortium composed of Scenedesmus sp. and Aspergillus niger , with a harvesting efficiency of 99.4%.…”

Section: Resultssupporting

confidence: 90%

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

et al. 2022

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In this study, a lichen-like structure was obtained through the production of a unique biomass, formed by algae cells of Scenedesmus obliquus adhering to the mycelium of filamentous fungal Mucor circinelloides. This structure was composed in two steps; in the first one, microalgal cells and spores were incubated separately, and in the second one, after 72 h of growth, isolated, mature mycelium was harvested and added to cell culture. For spores’ incubation, a culture medium containing only 2 g·L−1 of glucose and minerals was used. This culture medium, with low sugar content, provided a fungal biomass to the anchorage of microalgae cells. WC medium was used without and with sugarcane molasses supplementation for microalgae cells’ incubation. The lichen-type structure that was formed resulted in 99.7% efficiency in the recovery of microalgae cells and in up to 80% efficiency in the recovery of algae biomass in the lichen biomass composition. In addition, the resulting consortium attained a satisfactory lipid accumulation value (38.2 wt%) with a balanced fatty acid composition of 52.7% saturated plus monounsaturated fatty acids and 47.4% polyunsaturated fatty acids. Since fungal species are easy to recover, unlike microalgae, the lichen-like structure produced indicates an efficient low-cost bioremediation and harvesting alternative; in addition, it provides an oleaginous biomass for various industrial applications.

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Section: Resultssupporting

confidence: 90%

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

et al. 2022

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“…Meanwhile, mid-sized, loose, rough pellets (formed at 100 rpm) resulted in both internal and surface adsorption and therefore better harvesting. Similar effects of agitation on pellet morphology and its consequent harvesting efficiency have been well documented in other harvesting studies employing both pellet-assisted and spore-assisted methods (Bhattacharya et al 2017;Zhao et al 2019;Pei et al 2021). It is possible that agitation may affect pellet morphology and microalgal flocculation and harvesting similarly when filaments in suspension are used.…”

Section: Harvesting Efficiencysupporting

confidence: 69%

“…It is worth mentioning that temperature has a marked effect on harvesting efficiency. In general, temperature is directly correlated to harvesting efficiency, but only up to a maximal temperature specific to the fungal strain in question, after which it seems that heat stress damages the cells impeding harvesting (Bhattacharya et al 2017;Khothari et al 2017;Pei et al 2021). Despite the possible harvesting efficiency gains of increasing temperature, this would necessitate energy inputs resulting in increased operational costs.…”

Section: Harvesting Efficiencymentioning

confidence: 99%

“…Fungi employ, often simultaneously, several different microalgal harvesting mechanisms such as self-pelletization, excretion of EPS, attraction, entrapment (Egede et al 2016;Magdouli et al 2016), and attachment to the fungal cell wall (Wrede et al 2014;Du et al 2018). However, fungi are more attractive bioflocculants than bacteria because of their higher harvesting efficiencies (Zhou et al 2012;Li et al 2020;Nazari et al 2020), greater potential to improve total biomass yields and value due to their nutrient, enzyme, and biochemical content (Egede et al 2016;Li et al 2020), and larger, denser flocs (Nguyen et al 2019;Zhao et al 2019;Kim et al 2020;Pei et al 2021). Bacteria-microalgae flocs tend to be < 5 mm and less dense (Lee et al 2013;Nguyen et al 2019;Kim et al 2020;Pei et al 2021), while fungi-microalgae flocs have more densely packed structure and may grow to be > 5 mm (Zhao et al 2019;Kim et al 2020) improving settling and dewatering of the biomass (Benjamin and Lawler 2013).…”

Section: Introductionmentioning

confidence: 99%

“…However, fungi are more attractive bioflocculants than bacteria because of their higher harvesting efficiencies (Zhou et al 2012;Li et al 2020;Nazari et al 2020), greater potential to improve total biomass yields and value due to their nutrient, enzyme, and biochemical content (Egede et al 2016;Li et al 2020), and larger, denser flocs (Nguyen et al 2019;Zhao et al 2019;Kim et al 2020;Pei et al 2021). Bacteria-microalgae flocs tend to be < 5 mm and less dense (Lee et al 2013;Nguyen et al 2019;Kim et al 2020;Pei et al 2021), while fungi-microalgae flocs have more densely packed structure and may grow to be > 5 mm (Zhao et al 2019;Kim et al 2020) improving settling and dewatering of the biomass (Benjamin and Lawler 2013). As fungi can potentially add to the nutrient and biochemical value of the total biomass, there is no need to remove the fungal biomass after harvesting and therefore no contamination issues unlike bacteria (Egede et al 2016;Chu et al 2021a).…”

Section: Introductionmentioning

confidence: 99%

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Bioflocculation of Euglena gracilis via direct application of fungal filaments: a rapid harvesting method

et al. 2021

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The high cost and environmental impact of traditional microalgal harvesting methods limit commercialization of microalgal biomass. Fungal bioflocculation of microalgae is a promising low-cost, eco-friendly method but the range of fungal and microalgal species tested to date is narrow. Here, eight non-pathogenic, filamentous fungi were screened for their ability to self-pelletize and flocculate Euglena gracilis (ca.50 µm motile microalga) in suspension. Self-pelletization was tested under various rotational speeds, and species which formed pellets (Ø > 0.5 cm) were selected for harvesting tests. Filaments of each species were combined with E. gracilis at various ratios based on dry weight. Harvesting efficiency was determined by measuring the change in cell counts over time, and settling of the flocs was evaluated by batch settling tests. Three fungal species, Ganoderma lucidum, Pleurotus ostreatus, and Penicillium restrictum, were able to reliably flocculate and harvest 62–75% of the microalgae while leaving it unharmed. The results demonstrated that self-pelletization, harvesting, and settling were dependent on the fungal species. The fungi to algae ratio also had significant but contrasting effects on harvesting and settling. In balancing the needs to both harvest and settle the biomass, the optimal fungi to algae ratio was 1:2. The application of fungal filaments to microalgae in suspension produced readily settling flocs and was less time-consuming than other commonly used methods. This method is especially attractive for harvesting microalgal biomass for low-value products where speed, low cost, and cell integrity is vital.

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Fungal Exopolymeric Substances and Their Applications

Topal

2022

Fungal Biopolymers and Biocomposites

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Flocculation performance and mechanism of fungal pellets on harvesting of microalgal biomass

Cited by 59 publications

References 45 publications

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

Bioflocculation of Euglena gracilis via direct application of fungal filaments: a rapid harvesting method

Fungal Exopolymeric Substances and Their Applications

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