Flipping interferometry and its application for quantitative phase microscopy in a micro-channel

Roitshtain, Darina; Turko, Nir A.; Javidi, Bahram; Shaked, Natan T.

doi:10.1364/ol.41.002354

Cited by 70 publications

(28 citation statements)

References 15 publications

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“…Using the calibration factor obtained with the ARP sample we additionally obtained the quantitative phase shift induced by different parts of the HeLa cell. The maximum value of π/2 radians is in agreement with the literature [21,22], which is in the order of a few radians for this kind of cells.…”

Section: Hela Cell Experimentssupporting

confidence: 91%

Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

Tormo¹,

Khalenkow²,

Skirtach³

et al. 2019

OSA Continuum

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Raman spectroscopy probes the chemical composition of biological samples with sub-micron resolution, rendering it a powerful tool in the diagnosis of several diseases and the study unstained biological samples. However, the weak Raman signal leads to long acquisition times, unsuited to study dynamical processes or large samples. Quantitative phase microscopy can speed up the diagnosis, provide complementary data, and potentially link the Raman fingerprint of the sample with corresponding refractive indices. Here we demonstrate a 4π microscope that records both the Raman and quantitative phase information from the same sample spot.

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Section: Hela Cell Experimentssupporting

confidence: 91%

Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

Tormo¹,

Khalenkow²,

Skirtach³

et al. 2019

OSA Continuum

View full text Add to dashboard Cite

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“…Other CIM have been developed as well, such as the flipping interferometry unit proposed by Roitshtain et al [30]. This unit, as well as other units [31,32] produces holograms by means of interfering the sample beam with itself by overlaying an empty section of the image over the sample region of the image.…”

Section: Compact Interferometric Modules (Cim)mentioning

confidence: 99%

Label-Free Quantitative Imaging of Sperm for In-Vitro Fertilization Using Interferometric Phase MicroscopyIVF, IMSI, ICSI, IPM, Holography, Imaging, Sperm

Mirsky¹,

Barnea²,

Shaked³

2016

JFIV Reprod Med Genet

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Accurate morphological evaluation via imaging of sperm cells is a critical step in both routine sperm analysis and sperm cell selection for in vitro fertilization. In this paper, we review the significant advantages of interferometric phase microscopy (IPM), also called digital holographic microscopy, for label-free sperm imaging in fertility clinics. We first review the current state of the art of label-free sperm cell imaging for in vitro fertilization, including brightfield, Zernike phase contrast, differential interference contrast (DIC), Hoffman modulation contrast and polarized light microscopy, as well as intracytoplasmic morphologically selected sperm injection (IMSI). Using experimental demonstrations, we compare the limitations of these techniques for label-free sperm imaging. IPM and its advantages are then discussed in detail. These include the ability to receive quantitative data regarding the 3D structure of sperm cells, as well as data concerning sperm biomass and morphology without labeling. The current gap to clinical use is discussed, as well as the possible solution of employing compact and simple holographic modules, which may allow holographic imaging to reach the clinic in the near future.

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“…In the dynamic measurements, different environmental effects between the beams, namely, the individual mechanical vibration of optical components, inevitably trigger some remnant time-dependent noises which reduce the quality of * m-dashtdar@sbu.ac.ir retrieved images. Recently, many efforts have been devoted toward developing new settings with the higher phase sensitivity such as self-reference or common-path interferometers (CPIs), through which the phase stability of the system is significantly enhanced as the two overlapping beams travel along nearly equivalent paths [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]. Despite having the several advantages including simplicity and phase stability, many of the CPIs suffer from functional challenges which are imposed by loss of flux, inflexible off-axis angle, small field of view (FOV) limitation and precise optical alignment.…”

Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging based on Fresnel diffraction from a phase plate

Ebrahimi

Dashtdar

2019

Applied Physics Letters

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The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods are the subjects of growing interest. Here we introduce Fresnel diffraction from a phase step to the realm of quantitative phase imaging. By employing Fresnel diffraction of a divergent (or convergent) beam of light from a plane-parallel phase plate, we provide a viable, simple and compact platform for three-dimensional imaging of micron-sized specimens. The recorded diffraction pattern of the outgoing light from an imaging system in the vicinity of the plate edge can be served as a hologram, which would be analyzed via Fourier transform method to measure the sample phase information. The period of diffraction fringes is adjustable simply by rotating the plate without the reduction of both field of view and fringe contrast. The high stability of the presented method is affirmatively confirmed through comparison the result with that of conventional Mach-Zehnder based digital holographic method. Quantitative phase measurements on silica microspheres, onion skin and red blood cells ensure the validity of the method and its ability for monitoring nanometer-scale fluctuations of living cells, particularly in real-time.

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Flipping interferometry and its application for quantitative phase microscopy in a micro-channel

Cited by 70 publications

References 15 publications

Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

Label-Free Quantitative Imaging of Sperm for In-Vitro Fertilization Using Interferometric Phase MicroscopyIVF, IMSI, ICSI, IPM, Holography, Imaging, Sperm

Quantitative phase imaging based on Fresnel diffraction from a phase plate

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