Flexivirga lutea sp. nov., isolated from the faeces of a crested ibis, Nipponia nippon, and emended description of the genus Flexivirga

Abstract

Flexivirga lutea sp. nov., isolated from the faeces of a crested ibis, Nipponia nippon, and emended description of the genus Flexivirga , was isolated from the faeces of a crested ibis, Nipponia nippon. The phylogenetic analysis based on the 16S rRNA gene sequences showed that the closest relative of TBS-100T was Flexivirga alba DSM 24460 T with 97.11 % sequence similarity, and that strain TBS-100 T belonged to the genus Flexivirga. The optimum growth conditions for strain TBS-100 T were 30 C, at a pH of 7 and in the presence of 0 % (w/v) NaCl. The primary cellular fatty acids of strain TBS-100 T were anteiso-C 17 : 0 and iso-C 17 : 0 . The predominant isoprenoid quinones were MK-8 (H 4 ) (70.2 %) and MK-8 (H 6 ) (29.7 %). The polar lipids were diphosphatidylglycerol, phosphatidylinositol, seven unidentified lipids and an unidentified phospholipid. The whole-cell sugars of strain TBS-100 T were ribose, glucose, galactose, rhamnose and mannose. The peptidoglycan contained alanine, lysine, glutamic acid, glycine and aspartic acid. The DNA G+C content was 64.8 mol%. The phenotypic, phylogenetic and genotypic analyses indicated that strain TBS-100 T represents a novel species of the genus Flexivirga for which the name Flexivirga lutea sp. nov. is proposed. The type strain is TBS-100 T (=KCTC 39625

Endophytic Bacterium Flexivirga meconopsidis sp. nov. with Plant Growth-Promoting Function, Isolated from the Seeds of Meconopsis integrifolia

“…Genomic ANI and dDDH analyses, together with physiological and biochemical analyses, confirmed that strain Q11 T is a novel species of the genus Flexivirga. Compared with the reported strains of the genus Flexivirga [1][2][3][4][5][6], strain Q11 T has the abilities of nitrogen fixation (181.80 IU/L), IAA production (3.26 mg/L in 2 days), and plant growth promotion, this last function being described for the first time for the genus Flexivirga. Previous research showed that endophytes promote plant growth through nitrogen fixation, phosphorus dissolution, and growth hormone production [12,79].…”

“…The genus Flexivirga, a member of the Phylum Actinobacteria, was first described by Anzai et al [1]. The List of Prokaryotic Names with Standing in Nomenclature (https://lp sn.dsmz.de/search?word=Flexivirga, accessed on 8 October 2023) contains six species with legitimately recognized names, i.e., Flexivirga alba [1], Flexivirga endophytica [2], Flexivirga lutea [3], Flexivirga oryzae [4], Flexivirga caeni [5], and Flexivirga aerilata [6], which were isolated from the leaves of basil [2], the feces of ibis [3], the soil of a rice paddy [4], activated sludge [5], and an automobile air conditioning system [6], respectively. All members of the genus Flexivirga are described as Gram-positive bacteria, aerobic, non-motile, and with a high G + C content in their DNA (~67 mol%).…”