Flexible Octopus‐Shaped Hydrogel Particles for Specific Cell Capture

Chen, Lynna; An, Harry Z.; Haghgooie, Ramin; Shank, Aaron T.; Martel, Joseph M.; Toner, Mehmet; Doyle, Patrick S.

doi:10.1002/smll.201600163

Cited by 33 publications

(38 citation statements)

References 61 publications

(94 reference statements)

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“…The particle synthesis closely followed the previously reported protocol [30]. The functionalization process was identical for both particles synthesized by degassed mold lithography and stop flow lithography.…”

Section: Functionalization Of Hydrogel Microparticlementioning

confidence: 99%

“…Using functionalized polyethylene glycol (PEG) hydrogel microparticles for CTC capture is a promising technique in the affinity-based approaches [30,31]. These microparticles are comprised of a network of cross-linked polyethylene glycol diacrylate (PEGDA), which is biologically inert, and provide a water-like environment.…”

Section: Introductionmentioning

confidence: 99%

“…This platform enables control over the particle shape at high throughput synthesis efficiency. Chen et al reported that a multi-armed shape hydrogel microparticle could provide a flexible structure with increased surface area for CTC capture [30].…”

Section: Introductionmentioning

confidence: 99%

“…To increase the porosity of a PEG hydrogel, a low content of cross-linking monomer is used with a high content of porogen in the prepolymer [36][37][38]. This synthesis condition is challenging to apply using the previously described SFL fabrication technique [30], as SFL has a short UV exposure time to facilitate its high throughput and microfluidic system stability, which produces particles with poor network integrity and, occasionally, even failure in the formation of particle synthesis. Increasing the UV intensity to offset the reduced exposure time produces particles that lack uniformity and compromises the SFL system with increases in microfluidic malfunctions (i.e., device clogging) [39,40].…”

Section: Introductionmentioning

confidence: 99%

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Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

Lee

Maeng

Kim

et al. 2020

JCM

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Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs.

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Section: Functionalization Of Hydrogel Microparticlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

Lee

Maeng

Kim

et al. 2020

JCM

View full text Add to dashboard Cite

show abstract

“…[1][2][3][4] Owing to this merit of FL, new applications have emerged for in vitro diagnostics, [5][6][7][8][9][10][11][12][13] selfassembly of microparticles, 14,15 tissue engineering, [16][17][18] and drug delivery. 19 Materials that enable microfluidic devices to perform FL must satisfy two major requirements.…”

mentioning

confidence: 99%

Flow lithography in ultraviolet-curable polydimethylsiloxane microfluidic chips

Kim

Seo

et al. 2017

Biomicrofluidics

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Flow Lithography (FL) is the technique used for the synthesis of hydrogel microparticles with various complex shapes and distinct chemical compositions by combining microfluidics with photolithography. Although polydimethylsiloxane (PDMS) has been used most widely as almost the sole material for FL, PDMS microfluidic chips have limitations: (1) undesired shrinkage due to the thermal expansion of masters used for replica molding and (2) interfacial delamination between two thermally cured PDMS layers. Here, we propose the utilization of ultraviolet (UV)-curable PDMS (X-34-4184) for FL as an excellent alternative material of the conventional PDMS. Our proposed utilization of the UV-curable PDMS offers three key advantages, observed in our study: (1) UV-curable PDMS exhibited almost the same oxygen permeability as the conventional PDMS. (2) The almost complete absence of shrinkage facilitated the fabrication of more precise reverse duplication of microstructures. (3) UV-cured PDMS microfluidic chips were capable of much stronger interfacial bonding so that the burst pressure increased to $0.9 MPa. Owing to these benefits, we demonstrated a substantial improvement of productivity in synthesizing polyethylene glycol diacrylate microparticles via stop flow lithography, by applying a flow time (40 ms) an order of magnitude shorter. Our results suggest that UV-cured PDMS chips can be used as a general platform for various types of flow lithography and also be employed readily in other applications where very precise replication of structures on micro-or submicrometer scales and/or strong interfacial bonding are desirable. Published by AIP Publishing. [http://dx

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Advanced Bioresponsive Multitasking Hydrogels in the New Era of Biomedicine

Niazi

Alizadeh

Zarebkohan

et al. 2021

Adv Funct Materials

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Advanced forms of hydrogels have many inherently desirable properties and can be designed with different structures and functions. In particular, bioresponsive multifunctional hydrogels can carry out sophisticated biological functions. These include in situ single-cell approaches, capturing, analysis, and release of living cells, biomimetics of cell, tissue, and tumor-specific niches. They can allow in vivo cell manipulation and act as novel drug delivery systems, allowing diagnostic, therapeutic, vaccination, and immunotherapy methods. In the present review of multitasking hydrogels, new approaches and devices classified into point-of-care testing (POCT), microarrays, single-cell/rare cell approaches, artificial membranes, biomimetic modeling systems, nanodoctors, and microneedle patches are summarized. The potentials and application of each format are critically discussed, and some limitations are highlighted. Finally, how hydrogels can enable an "all-in-one platform" to play a key role in cancer therapy, regenerative medicine, and the treatment of inflammatory, degenerative, genetic, and metabolic diseases is being looked forward to.

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Flexible Octopus‐Shaped Hydrogel Particles for Specific Cell Capture

Cited by 33 publications

References 61 publications

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

Flow lithography in ultraviolet-curable polydimethylsiloxane microfluidic chips

Advanced Bioresponsive Multitasking Hydrogels in the New Era of Biomedicine

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