Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source

Kissenkötter, Jonas; Böhlken-Fascher, Susanne; Wahed, Ahmed Abd El

doi:10.3390/foods9101392

Cited by 3 publications

(2 citation statements)

References 27 publications

(28 reference statements)

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“…In this study, we found that only one SP used 4th GS with Oxford Nanopore technologies, and, accordingly, the full DNA barcodes were used (Ho et al., 2020). Until now, the available studies applying the Oxford Nanopore Technologies to food matrices especially rely on experimental mixtures to test the method efficiency (Kissenkotter et al., 2020; Rodríguez et al., 2023). No SPs used instead 3rd GS technologies.…”

Section: Resultsmentioning

confidence: 99%

Is the metabarcoding ripe enough to be applied to the authentication of foodstuff of animal origin? A systematic review

Giusti,

Malloggi,

Magagna

et al. 2023

Comp Rev Food Sci Food Safe

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Food authentication using molecular techniques is of great importance to fight food fraud. Metabarcoding, based on the next‐generation sequencing (NGS) technologies, allowing large‐scale taxonomic identification of complex samples via massive parallel sequencing of fragments (called DNA barcodes) simultaneously, has become increasingly popular in many scientific fields. A systematic review to answer the question “Is the metabarcoding ripe enough to be applied to the authentication of foodstuff of animal origin?” is presented. The inclusion criteria were focused on the selection of scientific papers (SPs) only applying metabarcoding to foodstuff of animal origin collected on the market. The 23 included SPs were first analyzed with respect to the metabarcoding phases: library preparation (target genes, primer pairs, and fragment length), sequencing (NGS platforms), and final data analysis (bioinformatic pipelines). Given the importance of primer selection, the taxonomic coverage of the used primers was also evaluated. In addition, the SPs were scored based on the use of quality control measures (procedural blanks, positive controls, replicates, curated databases, and thresholds to filter the data). A lack of standardized protocols, especially with respect to the target barcode/s and the universal primer/s, and the infrequent application of the quality control measures, leads to answer that metabarcoding is not ripe enough for authenticating foodstuff of animal origin. However, the observed trend of the SP quality improvement over the years is encouraging. Concluding, a proper protocol standardization would allow a wider use of metabarcoding by both official and private laboratories, enabling this method to become the primary for the authentication of foodstuffs of animal origin.

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Section: Resultsmentioning

confidence: 99%

Is the metabarcoding ripe enough to be applied to the authentication of foodstuff of animal origin? A systematic review

Giusti,

Malloggi,

Magagna

et al. 2023

Comp Rev Food Sci Food Safe

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“…This technique has been used extensively for microbial sequencing but less so for meat species identification. For this reason, Kissenkötter, Böhlken‐Fascher, and El Wahed (2020a) combined this technology with an offline BLAST search tool to identify horse, chicken, turkey, beef, sheep, duck, rabbit, goat, and donkey in meat products. In their research, the accuracy, sensitivity, and speed of the nanopore sequencing technology were established within the first hour of base calling.…”

Section: Dna‐based Methods For Game Species Identificationmentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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Disposable and instrument-free nucleic acid lateral flow cassette for rapid and on-site identification of adulterated goat milk

Wang,

Zhang,

Xiao

et al. 2024

Talanta

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Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source

Cited by 3 publications

References 27 publications

Is the metabarcoding ripe enough to be applied to the authentication of foodstuff of animal origin? A systematic review

Is the metabarcoding ripe enough to be applied to the authentication of foodstuff of animal origin? A systematic review

A systematic review of DNA‐based methods in authentication of game and less common meat species

Disposable and instrument-free nucleic acid lateral flow cassette for rapid and on-site identification of adulterated goat milk

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