Flea-Borne Rickettsioses in the North of Caldas Province, Colombia

Hidalgo, Marylin; Montoya, Viviana; Martínez, Alejandra; Mercado, Marcela; Ossa, Alberto de la; Vélez, Carolina; Estrada, Gloria Inés; Pérez, Jorge Enrique; Faccini-Martínez, Álvaro A.; Labruna, Marcelo B.; Valbuena, Gustavo

doi:10.1089/vbz.2012.1173

Cited by 36 publications

(33 citation statements)

References 20 publications

(20 reference statements)

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“…25 The finding is in agreement with the rickettsial infection identified in fleas of the same region in this study. The absence of R. typhi in our sample is probably because of the very small number of X. cheopis (the main vector for this bacterium) 41 collected in this study.…”

Section: Discussionsupporting

confidence: 92%

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Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Ramírez-Hernández

Montoya

Martínez

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Rickettsioses caused by Rickettsia felis are an emergent global threat. Historically, the northern region of the province of Caldas in Colombia has reported murine typhus cases, and recently, serological studies confirmed high seroprevalence for both R. felis and R. typhi. In the present study, fleas from seven municipalities were collected from dogs, cats, and mice. DNA was extracted and amplified by polymerase chain reaction (PCR) to identify gltA, ompB, and 17kD genes. Positive samples were sequenced to identify the species of Rickettsia. Of 1,341 fleas, Ctenocephalides felis was the most prevalent (76.7%). Positive PCR results in the three genes were evidenced in C. felis (minimum infection rates; 5.3%), C. canis (9.2%), and Pulex irritans (10.0%). Basic Local Alignment Search Tool (BLAST) analyses of sequences showed high identity values ( 98%) with R. felis, and all were highly related by phylogenetic analyses. This work shows the first detection of R. felis in fleas collected from animals in Colombia.

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Section: Discussionsupporting

confidence: 92%

“…A prospective arm of this study also corroborated different human infections with the aforementioned flea-borne rickettsial species. 25 The aim of this work was to detect, by molecular methods, the presence of Rickettsia species in fleas collected from animals in the urban area of seven municipalities from Caldas Province, Colombia.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Ramírez-Hernández

Montoya

Martínez

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…Older participants (aged 60+) had a 1.323 times lower odds of exposure to R. felis ( t = -2.095, P = 0.040; OR: 0.756; CI: 0.582–0.982), 1.202 times lower to R. typhi ( t = -1.93, P = 0.058; OR: 0.834, CI: 0.693–1.003), and 1.330 times lower to either R. felis or R. typhi (t = -2.147; P = 0.034; OR: 0.752; CI: 0.579–0.975) exposures, which is consistent with the findings of Hidalgo et al in a similar study in Spain [21]. In our study, actively working older participants spent less time (17.5 h) in private practice compared with their younger and middle-aged counterparts (22 and 24 h, respectively).…”

Section: Discussionsupporting

confidence: 91%

Serological evidence of exposure to Rickettsia felis and Rickettsia typhi in Australian veterinarians

et al. 2017

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Background Rickettsia felis and Rickettsia typhi are emerging arthropod-borne zoonoses causing fever and flu-like symptoms. Seroprevalence and risk factors associated with exposure to these organisms was explored in Australian veterinarians.MethodsOne hundred and thirty-one veterinarians from across Australia were recruited to participate in a cross-sectional survey. Veterinarians provided a single blood sample and answered a questionnaire on potential risk factors influencing their exposure to R. felis and R. typhi. Indirect microimmunofluorescence antibody testing (IFAT) was used to identify evidence of serological exposure of the participants to R. felis and R. typhi. Results were analyzed and a logistical regression model performed to predict risk factors associated with seropositivity.ResultsIn total, 16.0% of participants were seropositive to R. felis, 4.6% to R. typhi and 35.1% seropositive to both, where cross-reactivity of the IFAT between R. felis and R. typhi precluded a definitive diagnosis. Veterinarians residing within the south-eastern states of Victoria and Tasmania were at a higher risk of exposure to R. felis or generalised R. felis or R. typhi exposure. Older veterinarians and those that recommended flea treatment to their clients were found to be significantly protected from exposure.ConclusionsThe high exposure to R. felis amongst veterinary professionals suggests that flea-borne spotted fever is an important cause of undifferentiated fever conditions that may not be adequately recognized in Australia.

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“…Previous serosurveys have shown similar numbers of apparently healthy people in Colombia (18%), Spain (7%), Senegal (4%), and Kenya (3%) [7],[8] are seropositive. There were no differences in age or complete blood count (CBC) parameters between sero-positive and sero-negative people.…”

Section: Resultsmentioning

confidence: 94%

First report of Rickettsia felisin China

Zhang

Kelly

et al. 2014

BMC Infect Dis

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BackgroundRickettsia felis is a recently described flea-borne spotted fever group Rickettsia that is an emerging human pathogen. Although there is information on the organism from around the world, there is no information on the organism in China.MethodsWe used a commercial ELISA to detect antibodies reactive against R. felis in blood samples and developed a PCR to detect the gltA of the organism in blood samples and external parasites.ResultsWe found reactive antibodies in people (16%; 28/180), dogs (47%; 128/271) and cats (21%; 19/90) and positive PCRs with DNA from people (0.1%; 1/822), dogs (0.8%; 8/1,059), mice (10%; 1/10), ticks (Rhipicephalus sanguineus; 10%; 15/146), lice (Linognathus setosus; 16%; 6/37), fleas (Ctenocephalides felis felis; 95%; 57/60) and mosquitoes (Anopheles sinensis, Culex pipiens pallens; 6%; 25/428), but not from cats (0/135) or canine fecal swabs (0/43).ConclusionsThis is the first report of R. felis in China where there is serological and/ or PCR evidence of the organism in previously reported [people, dogs, cats, ticks (Rhipicephalus sanguineus), fleas (Ctenocephalides felis felis) and mosquitoes (Anopheles sinensis, Culex pipiens pallens)] and novel species [mice and lice (Linognathus setosus)].Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-014-0682-1) contains supplementary material, which is available to authorized users.

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Flea-Borne Rickettsioses in the North of Caldas Province, Colombia

Cited by 36 publications

References 20 publications

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Serological evidence of exposure to Rickettsia felis and Rickettsia typhi in Australian veterinarians

First report of Rickettsia felisin China

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