Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

Zhang, Zhiguo; Wang, Meilin; Xing, Shu; Zhang, Chen

doi:10.1016/j.fshw.2021.11.016

Cited by 21 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Afterward, the PVDF membranes were then incubated with diluted secondary antibodies for 1–2 h at room temperature. β-Actin was used as an internal reference protein, and PVDF membranes were developed and fixed to observe target protein expression changes ( 22 , 23 ).…”

Section: Methodsmentioning

confidence: 99%

“…Later, the protein transfer to the PVDF membrane was accomplished using an ice bath, the proteins were blocked by BSA for 1 h at room temperature, and the primary antibodies (Bcl-2, Bax, caspase-3) were added proportionally and incubated overnight at 4 • C. Afterward, the PVDF membranes were then incubated with diluted secondary antibodies for 1-2 h at room temperature. β-Actin was used as an internal reference protein, and PVDF membranes were developed and fixed to observe target protein expression changes (22,23).…”

Section: Western Blotmentioning

confidence: 99%

See 1 more Smart Citation

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

Feng

Zhou²,

Zhou³

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC50) were detected using the CCK-8 method, and the level of reactive oxygen species (ROS) was detected by using a DCFH-DA fluorescence probe. Finally, a Seahorse XFe24 energy analyzer (Agilent, United States) was used to detect cell mitochondrial pressure and glycolytic pressure. The results showed that TG could reduce the survival rate of HepG2 cells and that the IC50 level was 35.28 μg/ml. With increasing TG concentration, the level of ROS showed a concentration-dependent upward trend. Energy metabolism showed that each dose group of TG could significantly decline the mitochondrial respiratory and glycolytic functions of HepG2 cells. In conclusion, TG could significantly inhibit the mitochondrial respiration and glycolysis functions of HepG2 cells, increase the level of ROS, and inhibit cell proliferation. Thus, this experiment pointed out that Cistanche deserticola can be used as a source of anti-cancer foods or drugs in the future. However, further studies on its mechanisms and clinical applications are needed.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Western Blotmentioning

confidence: 99%

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

Feng

Zhou²,

Zhou³

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…Further, the fresh medium containing concentration-gradient apigenin and caspase inhibitor Z-VAD-FMK was added for 48 h followed by the addition of fresh medium containing MTT (5 mg/ml). Then, DMSO (150 μL) was added after 4 h and absorbance values were measured at 490nm and 630nm [20].…”

Section: Mtt Assay and Ldh Assaymentioning

confidence: 99%

The modulatory effect of oolong tea polyphenols on intestinal flora and hypothalamus gene expression in a circadian rhythm disturbance mouse model

Liang¹,

Zhong²,

Ma³

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

Apigenin, a natural flavonoid has been reported against a variety of cancer types. However, it is unclear whether apigenin can promote autophagy and ferroptosis in Ishikawa cells. There are few reports on the mechanism of apigenin on autophagy and ferroptosis of endometrial cancer Ishikawa cells. We found that iron accumulation, lipid peroxidation, GSH consumption, p62, HMOX1, and ferritin were increased, while, SLC7A11 and GPX4 were decreased. Fer-1, an iron-death inhibitor could reverse the effects of apigenin in Ishikawa cells. On the other hand, apigenin could promote autophagy via up-regulating Beclin 1, ULK1, ATG5, ATG13, and LC3B and down-regulating AMPK, mTOR, P70S6K, and ATG4. Furthermore, apigenin could inhibit tumor tissue proliferation and restrict tumor growth via ferroptosis in vivo.

show abstract

“…Rosa rugosa Thunb., a traditional Chinese medicinal material to beautify skin and relieve skin aging, which can also be used to treat diarrhea, dysmenorrhea, cardiovascular, and cerebrovascular diseases [7]. Rosa rugosa Thunb.…”

Section: Introductionmentioning

confidence: 99%

“…Searching for natural antioxidants and tyrosinase inhibitors has been a hot topic in cosmetic industry research. Rosa rugosa Thunb., a traditional Chinese medicinal material to beautify skin and relieve skin aging, which can also be used to treat diarrhea, dysmenorrhea, cardiovascular, and cerebrovascular diseases [7]. Rosa rugosa Thunb.…”

Section: Introductionmentioning

confidence: 99%

High‐resolution monophenolase/diphenolase/radical scavenging profiling for rapid screening of natural whitening candidates from Rosa rugosa cv. ‘Plena’

Wen

Quan

Qiu

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

Antioxidants and tyrosinase inhibitory components were successfully screened and separated from Rosa rugosa cv. 'Plena' by high-performance liquid chromatography microfractionation bioactive screening combined with several separation and purification methods. Ethyl acetate extract of Rosa rugosa cv.'Plena' showed high antioxidant activity and tyrosinase inhibitory activity. Highspeed countercurrent chromatography, silica gel column chromatography, and semi-preparative high-performance liquid chromatography were used for the preparative separation of four bioactive components from ethyl acetate extract. Two tyrosinase-inhibiting active substances, flavogallonic acid, and N 1 -N 5 -N 10tri-4-p-coumaroylspermidine, were isolated from Rosa rugosa cv. 'Plena', and they showed great monophenolase inhibition activity (half-maximal inhibitory concentration: 664.60 and 23.77 μg/ml, respectively) and excellent diphenolase inhibition activity (half-maximal inhibitory concentration: 23 614.61 and 16.80 μg/ml, respectively). Meanwhile, gallic acid, flavogallonic acid, and ellagic acid were shown to have excellent 1,1-diphenyl-2-picryl-hydrazyl antioxidant activity (half maximal inhibitory concentration: 6.66, 20.17, and 13.45 μg/ml), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant activity (half maximal inhibitory concentration: 3.53, 3.83, and 2.78 μg/ml). Molecular docking revealed that flavogallonic acid and N 1 -N 5 -N 10 -tri-4-p-coumaroylspermidine had a strong binding affinity (-9.3 and -10 kcal/mol, respectively) to tyrosinase through hydrogen bonding and hydrophobic interactions.

show abstract

Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

Cited by 21 publications

References 36 publications

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

The modulatory effect of oolong tea polyphenols on intestinal flora and hypothalamus gene expression in a circadian rhythm disturbance mouse model

High‐resolution monophenolase/diphenolase/radical scavenging profiling for rapid screening of natural whitening candidates from Rosa rugosa cv. ‘Plena’

Contact Info

Product

Resources

About