Flavonoids composition and antioxidant potential assessment of extracts from Gannanzao Navel Orange (<i>Citrus sinensis</i> Osbeck Cv. Gannanzao) peel

Long, Xiaoyan; Zeng, Xiaogang; Yan, Huiting; Xu, Min; Zeng, Qingting; Xu, Changwen; Xu, Qingmei; Liang, Yan; Zhang, Jun

doi:10.1080/14786419.2019.1593162

Cited by 38 publications

(23 citation statements)

References 13 publications

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“…This trend was in accordance with the previous finding by Anagnostopoulou et al ., showing that the ethyl acetate extract accumulated the largest amount of TPC in comparison with the other extraction solvents, including diethyl ether, n ‐butanol, and water. In agreement with the present results, the ethyl acetate extract of Gannanzao navel orange peel exhibited the highest TPC (83.36 GAE mg g −1 DW) when compared with 95% ethanol, petroleum ether, and water extracts . The TPC of 80% methanol / water extract of C. sinensis var.…”

Section: Resultssupporting

confidence: 91%

“…The peel extracts of Gannanzao navel orange ( Citrus sinensis Osbeck cv. Gannanzao), a bud mutation of Newhall, exhibited similar antioxidant capacities to the present study, with IC 50 being 38.33, 202.33, 586.08, and 378.93 μg mL −1 for the ethyl acetate, petroleum ether, water and 95% ethanol extracts, respectively, in the DPPH assay . Ghasemi et al .…”

Section: Resultssupporting

confidence: 83%

“…Gannanzao), a bud mutation of Newhall, exhibited similar antioxidant capacities to the present study, with IC 50 being 38.33, 202.33, 586.08, and 378.93 μg mL −1 for the ethyl acetate, petroleum ether, water and 95% ethanol extracts, respectively, in the DPPH assay. 32 Ghasemi et al reported that the methanolic extract of Washington navel orange peel (Citrus sinensis var. Washington), a cultivar close to Newhall navel orange, had antioxidant activity to scavenge DPPH radical with IC 50 being 1100 μg mL −1 , 33 which was lower than EE (304.50 μg mL −1 ), especially EtOAcE (IC 50 23.70 μg mL −1 ) in the present study.…”

Section: Antioxidant Testmentioning

confidence: 99%

“…In agreement with the present results, the ethyl acetate extract of Gannanzao navel orange peel exhibited the highest TPC (83.36 GAE mg g −1 DW) when compared with 95% ethanol, petroleum ether, and water extracts. 32 The TPC of 80% methanol / water extract of C. sinensis var. Washington Navel peel was 18.71 GAE mg −1 DW, 4 which was less than the present result of TPC in EE (30.62 GAE mg g −1 DW).…”

Section: Tpc and Tfc Analysismentioning

confidence: 99%

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Chemical composition, antioxidant, antibacterial, and tyrosinase inhibition activity of extracts from Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) peel

et al. 2020

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BACKGROUND Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) is the major navel orange cultivar planted in China. Almost all Newhall navel orange peels produced in that country are currently discarded, which is not only harmful to the environment but also a waste of resources. It is therefore necessary to carry out research to explore the utilization potential of this resource. RESULTS A 95% ethanol extract (EE) of Newhall orange peel was prepared and fractionated into three subextracts: petroleum ether extract (PEE), ethyl acetate extract (EtOAcE), and water extract (WE) by simple liquid / liquid extractions. These four extracts were then subjected to antioxidant, antibacterial, and tyrosinase inhibition assays. Interestingly, EtOAcE was significantly superior to all other three extracts, exhibiting the best biological effects. The total polyphenol content (TPC), total flavonoid content (TFC), and primary individual flavonoids of these four extracts were analyzed and compared. This was followed by principal component analysis (PCA) and the Pearson's correlation test. The result indicates that the primary bioactive compounds responsible for the biological effects of the EtOAcE are sinensetin, 4′,5,6,7‐tetramethoxyflavone, nobiletin, 3,3′,4′,5,6,7‐hexamethoxyflavone, and narirutin. In view of its easy preparation and potent biological effects, EtOAcE might demonstrate excellent application potential in various industrial areas. CONCLUSIONS This study successfully identified EtOAcE as a potent naturally occurring antioxidant, antibacterial, and tyrosinase inhibitory agent, which might add value to the utilization of Newhall navel orange peel in the food, cosmetic, and pharmaceutical industries. © 2020 Society of Chemical Industry

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 83%

Section: Antioxidant Testmentioning

confidence: 99%

Section: Tpc and Tfc Analysismentioning

confidence: 99%

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Chemical composition, antioxidant, antibacterial, and tyrosinase inhibition activity of extracts from Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) peel

et al. 2020

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“…Given that Isoori belongs to flavonoids that have been reported to have antioxidant activity 38,39 , its effects on the oxidative stress indicators like glutathione synthetase (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and ascorbic acid (VC) levels were also investigated. Compared to the vehicle solution, Isoori effectively increased the concentrations of GSH, SOD, and VC in paws injected with formalin, but suppressed formalin stimulated MDA production in a dose-dependent manner ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Pathway-specific enzymes from bamboo and crop leaves biosynthesize anti-nociceptive C-glycosylated flavones

et al. 2020

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C-glycosylated flavones (CGFs) are promising candidates as anti-nociceptive compounds. The leaves of bamboo and related crops in the grass family are a largely unexploited bioresource with a wide array of CGFs. We report here pathway-specific enzymes including Cglycosyltransferases (CGTs) and P450 hydroxylases from cereal crops and bamboo species accumulating abundant CGFs. Mining of CGTs and engineering of P450s that decorate the flavonoid skeleton allowed the production of desired CGFs (with yield of 20-40 mg/L) in an Escherichia coli cell factory. We further explored the antinociceptive activity of major CGFs in mice models and identified isoorientin as the most potent, with both neuroanalgesic and antiinflammatory effects superior to clinical drugs such as rotundine and aspirin. Our discovery of the pain-alleviating flavonoids elicited from bamboo and crop leaves establishes this previously underutilized source, and sheds light on the pathway and pharmacological mechanisms of the compounds.

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High‐performance liquid chromatography micro‐fraction bioactive evaluation combined with countercurrent chromatographic separation of antioxidants from Citrus peel and their tyrosinase inhibition activities

Fang

Lin

Chen

et al. 2023

J of Separation Science

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In the present study, high-performance liquid chromatography micro-fraction bioactive evaluation and high speed countercurrent chromatography were performed on screening, identification and isolation of antioxidants from Citrus peel. Three compounds were screened as antioxidants and tyrosinase inhibitors using 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) radical cation scavenging assay and tyrosinase activity test, then they were identified as eriocitrin, narirutin and hesperidin. Moreover, the solvent system ethyl acetate-n-butanolwater (6:4:10, v/v/v) was used for separation of ethyl acetate extract of Citrus peel by high speed countercurrent chromatography. In total, 0.45 mg of eriocitrin with 87.10% purity, 2.04 mg of narirutin with 95.19% purity and 1.35 mg of hesperidin with 95.19% purity were obtained from 20 mg of ethyl acetate extract of Citrus peel in a single run and then each component was subjected to 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation scavenging assay and tyrosinase inhibition assay. Eriocitrin showed great antioxidant activity (the half-maximum concentration: 3.65 µM) and tyrosinase inhibition activity (the half-maximum concentration: 115.67 µM), while narirutin and hesperidin exhibited moderate activity. Tyrosinase inhibition activity for eriocitrin in vitro was reported for the first time. Furthermore, molecular docking between eriocitrin and mushroom tyrosinase was also studied.

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Flavonoids composition and antioxidant potential assessment of extracts from Gannanzao Navel Orange (Citrus sinensis Osbeck Cv. Gannanzao) peel

Cited by 38 publications

References 13 publications

Chemical composition, antioxidant, antibacterial, and tyrosinase inhibition activity of extracts from Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) peel

Chemical composition, antioxidant, antibacterial, and tyrosinase inhibition activity of extracts from Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) peel

Pathway-specific enzymes from bamboo and crop leaves biosynthesize anti-nociceptive C-glycosylated flavones

High‐performance liquid chromatography micro‐fraction bioactive evaluation combined with countercurrent chromatographic separation of antioxidants from Citrus peel and their tyrosinase inhibition activities

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