Flavonoids: a colorful model for the regulation and evolution of biochemical pathways

Koes, Ronald; Verweij, Walter; Quattrocchio, Francesca

doi:10.1016/j.tplants.2005.03.002

Cited by 1,391 publications

(1,181 citation statements)

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“…Further physiological and molecular investigations of the identified mutant allele will help to elucidate the control of developmental transitions in facultative long-day petunia. Anthocyanin biosynthesis and regulation have been intensively studied in petunia (Winkel-Shirley 2001;Koes et al 2005;Ambawat et al 2013). Genetic analyses of flower color mutants have been instrumental to the identification of several structural and regulatory genes in the species (Koes et al 2005;Ambawat et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Anthocyanin biosynthesis and regulation have been intensively studied in petunia (Winkel-Shirley 2001;Koes et al 2005;Ambawat et al 2013). Genetic analyses of flower color mutants have been instrumental to the identification of several structural and regulatory genes in the species (Koes et al 2005;Ambawat et al 2013). In a large number of higher plant species, the activity of chalcone synthase displays gene redundancy and it is encoded by at least nine genes in Medicago truncatula, three in grapevine, two in maize and only one in Antirrhinum (Desnos et al 2001;Koes et al 2005;Abe and Morita 2010).…”

Section: Discussionmentioning

confidence: 99%

“…1d, Table S4). All classes of transcription factor families demonstrated to be involved in the regulation of the pathway in Arabidopsis and maize were found in petunia, namely; WRYK, WD-40, MADS, MYB, HLH and Zinc finger (Koes et al 2005;Ambawat et al 2013) (Fig. 1d, Table S4).…”

Section: Data Mining For Photoperception Light-signaling and Anthocymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Anthocyanin biosynthesis occurs via a branch of the flavonoid pathway and consists of a well-known sequence of enzymatic steps, studied in Antirrhinum majus, A. thaliana, Petunia and Zea mays (Koes et al 2005). The activity of anthocyanin biosynthetic genes is largely regulated at the transcriptional level via the function of several classes of transcriptional regulators, such as: WD40, helix-loop-helix (HLH), R2R3 and R3 MYB, WRYK, Zn finger and MADSbox (Koes et al 2005;Ambawat et al 2013). In several plant species, light has been demonstrated to be an important environmental factor controlling anthocyanin accumulation, directly via transcriptional control of biosynthetic genes and transcription factors or indirectly via developmental regulation (Albert et al 2009;Lopez et al 2012;Petroni and Tonelli 2011;Huang et al 2012;Ambawat et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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A reverse genetics approach identifies novel mutants in light responses and anthocyanin metabolism in petunia

Berenschot

Quecini

2013

Physiol Mol Biol Plants

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Flower color and plant architecture are important commercially valuable features for ornamental petunias (Petunia x hybrida Vilm.). Photoperception and light signaling are the major environmental factors controlling anthocyanin and chlorophyll biosynthesis and shadeavoidance responses in higher plants. The genetic regulators of these processes were investigated in petunia by in silico analyses and the sequence information was used to devise a reverse genetics approach to probe mutant populations. Petunia orthologs of photoreceptor, light-signaling components and anthocyanin metabolism genes were identified and investigated for functional conservation by phylogenetic and protein motif analyses. The expression profiles of photoreceptor gene families and of transcription factors regulating anthocyanin biosynthesis were obtained by bioinformatic tools. Two mutant populations, generated by an alkalyting agent and by gamma irradiation, were screened using a phenotype-independent, sequence-based method by high-throughput PCR-based assay. The strategy allowed the identification of novel mutant alleles for anthocyanin biosynthesis (CHALCONE SYNTHASE ) and regulation (PH4), and for light signaling (CONSTANS ) genes.

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