Colouring up Plant Biotechnology

Martin, Cathie; Zhang, Yang; Tomlinson, Laurence; Kallam, Kalyani; Luo, Jie; Jones, Jonathan D. G.; Granell, Antonio; Orzáez, Diego; Butelli, Eugenio

doi:10.1002/9781118299753.ch5

Cited by 4 publications

(4 citation statements)

References 45 publications

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“…We used the promoter of the PLI gene, which is induced by light and is active mainly in tomato skin (Estornell et al ., ), to drive the expression of the MYB transcriptional factor Rosea 1 . (Martin et al ., ) We expressed PLI:Ros1 together with 35S:Del in tomato using a binary vector that carried both gene constructs (Martin et al ., ). The new PLI:Ros1/35S:Del (PRD) lines accumulated high levels of anthocyanins in fruit skin, with much less anthocyanin in the fruit flesh compared with previously reported E8:Del/Ros1 lines (Butelli et al ., ).…”

Section: Resultsmentioning

confidence: 99%

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Accumulation of anthocyanins in tomato skin extends shelf life

et al. 2013

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SummaryShelf life is one of the most important traits for the tomato (Solanum lycopersicum) industry. Two key factors, post-harvest over-ripening and susceptibility to post-harvest pathogen infection, determine tomato shelf life.Anthocyanins accumulate in the skin of Aft/Aft atv/atv tomatoes, the result of introgressing alleles affecting anthocyanin biosynthesis in fruit from two wild relatives of tomato, which results in extended fruit shelf life. Compared with ordinary, anthocyanin-less tomatoes, the fruits of Aft/ Aft atv/atv keep longer during storage and are less susceptible to Botrytis cinerea, a major tomato pathogen, post-harvest.Using genetically modified tomatoes over-producing anthocyanins, we confirmed that skinspecific accumulation of anthocyanins in tomato is sufficient to reduce the susceptibility of fruit to Botrytis cinerea.Our data indicate that accumulation of anthocyanins in tomato fruit, achieved either by traditional breeding or genetic engineering can be an effective way to extend tomato shelf life.

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Section: Resultsmentioning

confidence: 99%

“…Using Gateway recombination, the PLI promoter was introduced into pDONR 207 to create pENTR‐PLI. The PLI promoter was then inserted into the binary vector pJAM1890 (GATEWAY:Ros1/35S:Del) (Martin et al ., ) to create pPLI:Ros1/35S:Del (pPRD). pPRD was transferred to Agrobacterium tumefaciens strain AGL1 by triparental mating.…”

Section: Methodsmentioning

confidence: 99%

Accumulation of anthocyanins in tomato skin extends shelf life

et al. 2013

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“…To this end, pX11-Mt was crossed with a 35S::Del/PNH::Ros1 tomato line (in the cv. MicroTom background), which accumulates anthocyanins in fruit and vegetative tissues (25). The cross resulted in generation of plants that produce both anthocyanins and betalains, as could be seen by their distinct fruit pigmentation (Fig.…”

Section: Fig 1 Representation Of the Betalain Biosynthetic Pathway mentioning

confidence: 99%

Engineered gray mold resistance, antioxidant capacity, and pigmentation in betalain-producing crops and ornamentals

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Grossman

Vela‐Corcía

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

129

111

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Betalains are tyrosine-derived red-violet and yellow plant pigments known for their antioxidant activity, health-promoting properties, and wide use as food colorants and dietary supplements. By coexpressing three genes of the recently elucidated betalain biosynthetic pathway, we demonstrate the heterologous production of these pigments in a variety of plants, including three major food crops: tomato, potato, and eggplant, and the economically important ornamental petunia. Combinatorial expression of betalain-related genes also allowed the engineering of tobacco plants and cell cultures to produce a palette of unique colors. Furthermore, betalain-producing tobacco plants exhibited significantly increased resistance toward gray mold (), a pathogen responsible for major losses in agricultural produce. Heterologous production of betalains is thus anticipated to enable biofortification of essential foods, development of new ornamental varieties, and innovative sources for commercial betalain production, as well as utilization of these pigments in crop protection.

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“…Most higher plants can synthesize anthocyanins, but the exact nature of the anthocyanins formed can differ widely in plant species due to the activity of specific enzymes that add sugars, methyl groups, and acyl residues to the basic anthocyanidin structure [ 6 ]. Anthocyanin biosynthesis is generally catalyzed by a total of nine conserved enzymes: phenylalanine ammonium-lyase (PAL), cinnamate 4-hydroxylase (C4H), p -coumaroyl 4-CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS).…”

Section: Introductionmentioning

confidence: 99%

Regulation of Anthocyanin Biosynthesis by Drought and UV-B Radiation in Wild Tomato (Solanum peruvianum) Fruit

et al. 2022

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Anthocyanins are plant pigments derived from the phenylpropanoid pathway which are produced in many different species, contributing to defense against stresses by their antioxidant properties. Cultivated tomatoes cannot synthesize flavonoids; however, wild tomatoes such as Solanum chilense and Solanum lycopersicoides have anthocyanin pigmented skin. Other wild tomato species such as Solanum peruvianum have been poorly studied concerning anthocyanin accumulation in the fruit. This research is the first to address the regulation of anthocyanin biosynthesis mediated by drought stress and light radiation in S. peruvianum fruit. Transcript accumulation of SpAN2, encoding for a key MYB type transcription factor for the regulation of anthocyanin biosynthesis, was induced in the fruit of plants exposed to drought treatment. In addition, fruit peel accumulates a greater anthocyanin content in water deficit-treated plants. The expression of SpAN2 was also regulated according to sunlight exposure, reaching a higher expression during maximal daily UV radiation and under controlled UV-B treatments. Similar results were observed for the expression of the late flavonoid biosynthetic gene dihydroflavonol 4-reductase (SpDFR). These results suggest that SpAN2 and SpDFR are involved in anthocyanin biosynthesis under drought stress and UV radiation in S. peruvianum.

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Colouring up Plant Biotechnology

Cited by 4 publications

References 45 publications

Accumulation of anthocyanins in tomato skin extends shelf life

Accumulation of anthocyanins in tomato skin extends shelf life

Engineered gray mold resistance, antioxidant capacity, and pigmentation in betalain-producing crops and ornamentals

Regulation of Anthocyanin Biosynthesis by Drought and UV-B Radiation in Wild Tomato (Solanum peruvianum) Fruit

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