Flavonoid compounds from pollen and stigma of Petunia hybrida: Inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid

Zerback, Rainer; Dressler, K.; Heß, Dieter

doi:10.1016/0168-9452(89)90192-1

Cited by 63 publications

(26 citation statements)

References 27 publications

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“…In addition, polycyclic compounds, such as chalcones, coniferyl alcohol, and flavonoids and their glucosides, also have an inducing activity (26,32,45). Recently, it was suggested that virulence-inducing acetophenones, such as acetosyringone, are restricted to members of the family Solanaceae (33).…”

mentioning

confidence: 99%

Growth inhibition and loss of virulence in cultures of Agrobacterium tumefaciens treated with acetosyringone

1992

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Acetosyringone, a phenolic inducer of the virulence (vir) genes of Agrobacterium tumefaciens, inhibited the growth of the nopaline-type strains T37 and C58 incubated under acidic conditions. In the course of a 6-day incubation with acetosyringone, avirulent clones were produced in different proportions by strains T37 and C58 and also by a spontaneous variant of strain C58, denominated C58F. The proportion of avirulent clones in acetosyringone-treated cultures often exceeded 50%o for strains T37 and C58F and was of the order of 1% for strain C58. Control cultures not exposed to acetosyringone did not yield avirulent clones. Two other vir inducers, sinapinic acid and syringaldehyde, also inhibited growth and promoted accumulation of avirulent clones in cultures of strains C58F and T37. On the other hand, various acetosyringone analogs reported not to induce the vir genes did not act as growth inhibitors. All of the T37 and most of the C58F avirulent clones examined still carried a Ti plasmid. In all instances examined, avirulent clones still carrying a Ti plasmid were mutated in this plasmid. Mutants of strain C58F lacked the capacity to induce a virB::lacZ fusion in the presence of acetosyringone.The causal agent of crown gall, Agrobacterium tumefaciens, infects plant wounds where it detects the presence of specific compounds of plant origin. The virulence (vir) genes present on the Ti plasmids ofA. tumefaciens are then induced. Expression of the vir genes in the bacterium leads to the transfer to host plant cells of a particular region of the Ti plasmids, called the transferred (T-) DNA. This DNA of bacterial origin becomes stably integrated into the nuclear genome of the plant cells.

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confidence: 99%

Growth inhibition and loss of virulence in cultures of Agrobacterium tumefaciens treated with acetosyringone

1992

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“…Pollen flavonoids have been detected in several species, where they impart a distinctive yellow color to pollen and can account for a large percentage (2-4%) of the dry weight (8)(9)(10). Analyses of petunia-pollen extracts have identified the major flavonoids as 3-0-glycosides of kaempferol and quercetin, 4,2',4',6'-tetrahydroxychalcone, and a dihydroflavonol, taxifolin (10)(11)(12). Maize pollen contains at least 10 glycosides of kaempferol, quercetin, and isorhamnetin (13).…”

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confidence: 99%

Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen.

Nagel

Taylor

1992

Proc. Natl. Acad. Sci. U.S.A.

416

373

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Chalcone synthase catalyzes the initial step of that branch of the phenylpropanoid pathway that leads to flavonoids. A lack of chalcone synthase activity has a pleiotropic effect in maize and petunia mutants: pollen fertility as well as flavonoid synthesis is disrupted. Both maize and petunia mutants are self-sterile due to a failure to produce a functional pollen tube. The finding that the mutant pollen is partially functional on wild-type stigmas led to the isolation and identification of kaempferol as a pollen germination-inducing constituent in wild-ype petunia stigma extracts. We show that adding micromolar quantities of kaempferol to the germination medium or to the stigma at pollination is sufficient to restore normal pollen germination and tube growth in vitro and full seed set in vivo. Further we show that the rescue ability resides in particular structural features of a single class of compounds, the flavonol aglycones. This rmding identifies another constituent of plant reproduction and suggests that addition or removal of the flavonol signal during pollen germination and tube growth provides a feasible way to control plant fertility.

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“…The candidates included phenolic compounds such as kaempferol and cinnamaldehydes. The families of kaempferol and hydroxycinnamides are associated with vir gene induction (Zerback et al, 1989;Berthelot et al, 1998;Joubert et al, 2004). Some candidate metabolites had no relationship with the interaction between Agrobacterium and soybeans.…”

Section: Candidate Metabolitesmentioning

confidence: 99%

Relationship of Transformation Efficiency and Metabolites Induced in Korean Soybean Cotyledons Treated with Sonication

Song¹,

Yim²,

Jung³

et al. 2013

Korean Journal of Crop Science

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The interaction between Agrobacterium and soybean has been studied at the transcriptome level but not at the metabolic level. However, it is necessary to investigate the difference in metabolites between susceptible and non-susceptible cultivars for high efficiency transformation. We investigated the difference in metabolites from sonicated soybean cotyledons of Korean cultivars and Bert cultivar. To identify difference in metabolites, sonicated extracts were analysed by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS). The soybean cultivars were classified by susceptibility using green fluorescent protein expression. We found a difference in metabolites between the high susceptible and low susceptible cultivars. The FT-ICR/MS experimental m/z data of different metabolites were compared with theoretical m/z in KNApSAcK database. The candidate list was made using KNApSAcK and focused on phenolic compounds. These candidate metabolites are speculated to influence factors in the interaction. This list of candidates may be useful to investigate the interaction between Agrobacterium and plants to increase transformation efficiency.

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Flavonoid compounds from pollen and stigma of Petunia hybrida: Inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid

Cited by 63 publications

References 27 publications

Growth inhibition and loss of virulence in cultures of Agrobacterium tumefaciens treated with acetosyringone

Growth inhibition and loss of virulence in cultures of Agrobacterium tumefaciens treated with acetosyringone

Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen.

Relationship of Transformation Efficiency and Metabolites Induced in Korean Soybean Cotyledons Treated with Sonication

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