Flavonoid biosynthesis in flower petals of five lines of lisianthus (Eustoma grandiflorum Grise.)

Davies, Kevin; Bradley, Jenna; Schwinn, Kathy E.; Markham, Kenneth R.; Podivinsky, Ellen

doi:10.1016/0168-9452(93)90080-j

Cited by 56 publications

(37 citation statements)

References 22 publications

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“…In Japanese morning glory, c and ca mutations conferring acyanic flowers were frameshift mutations caused by a 2-bp deletion and 7-bp insertions in the genes encoding the R2R3-MYB and WDR transcriptional regulators, respectively (Morita et al, 2006), and a-3 and Sp-1, which cause unstable acyanic flowers, resulted from a transposon insertion in the DFR gene and CHI gene, respectively (Iida et al, 1999). Davies et al (1993) analyzed the expression of some anthocyanin biosynthetic genes in the flowers of five lisianthus lines, including two acyanic flower lines. It was reported that DFR gene expression was decreased in one acyanic flower line and that neither of the two acyanic flower lines had ANS activity.…”

Section: Discussionmentioning

confidence: 99%

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Shimizu

Ohnishi

Morikawa

et al. 2011

J. Japan. Soc. Hort. Sci.

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We studied the genomic sequence of the anthocyanidin synthase (ANS) gene in acyanic flower lines of lisianthus and detected a 94-bp deletion of the sequence in comparison with that of cyanic flower lines. Cross-pollination was made between acyanic and cyanic flower lines to yield F 1 progeny, followed by its self-pollination. The segregated individuals between cyanic and acyanic flowers among the F 2 population were of 24 and 8, respectively (3 : 1), which suggested that one gene of the F 1 hybrid was unequivocally involved in anthocyanin production. The homozygous ANS genes that have a set of 94-bp deletion co-segregated a recessive phenotype of white color in the F 2 population. Genomic PCR of the ANS genes of the 14 independent lisianthus lines of acyanic flowers, such as white, yellow, pale yellow, and pale green flowers, revealed that all possessed a homozygous 94-bp deletion. The data support that the acyanic flower colors are associated with a 94-bp deletion in the ANS gene as a result of the loss of anthocyanin accumulation.

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Section: Discussionmentioning

confidence: 99%

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Shimizu

Ohnishi

Morikawa

et al. 2011

J. Japan. Soc. Hort. Sci.

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“…Common regulatory factors controlling the expression of genes in the anthocyanin biosynthesis pathway are the key for colorations in flowers and other specific tissues (Davies et al, 1993). Analysis of white flowers in Sim carnation and gentians in comparison to their colored-flower counterparts suggested that mutations in regulatory proteins could result in the failure to induce a set of anthocyanin production genes during flower development (Mato et al, 2000;Nakatsuka et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of Sequence and Expression of Eight Anthocyanin Biosynthesis Genes on Floral Coloration in Four Dendrobium Hybrids

et al. 2015

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Understanding the control of anthocyanin biosynthesis is beneficial to genetic improvement for floral production in Dendrobium orchids. Full-length cDNA of CHS, CHI1, CHI2, F3H, DFR, ANS, F3'5'H, and FLS was isolated from Dendrobium hybrids with purple, peach, white and greenish white flowers. Analysis of the deduced amino acid sequences and gene expression levels of the eight genes suggested potential causes of color variation among the hybrids. Peach hybrid (SC) was likely due to changes in anthocyanin production from cyanidin to pelargonidin through mutations in F3'H, and the low color intensity was likely derived from the low expression levels of CHI1 and CHI2. In addition, white hybrid (RW) was likely caused by several mutations in F3H and/or high expression levels of FLS, an enzyme that converts color flavonoid intermediates into colorless flavonols. Simultaneous loss of F3H, DFR, and ANS expression observed in another white hybrid (JW) indicated that an alteration of anthocyanin regulatory controls was likely the cause of white coloration. Furthermore, analysis of hybrid mutants bearing pale and dark flowers demonstrated the influence of the expression of anthocyanin genes on the intensity of flower colors. Data obtained from this work could contribute to new strategies for future orchid breeding.

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“…Group III includes F3H (flavanone 3-hydroxylase), F3'5'H,, ANS (anthocyanidin synthase), , and 5AT (anthocyanin 5-aromatic acyltransferase), which are all expressed in the late developmental stages. Davies et al (1993) observed that PAL (phenylalanine ammonia-lyase), CHS, and CHI are expressed at all stages of lisianthus flower development, while DFR is expressed only at the late stages. Noda et al (2004) also reported that lisianthus CHS, CHI, and F3H were coordinately expressed during all stages of floral development, whereas F3'5'H, ANS, and DFR were expressed in late stages and FLS (flavonol synthase) was expressed in early stages.…”

Section: Transgenic Complementation Of I Nil With Lisianthusmentioning

confidence: 98%

Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H

et al. 2015

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A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar 'Violet', which lacks a functional F3'H gene. In addition, the transgenic 'Violet' plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

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Flavonoid biosynthesis in flower petals of five lines of lisianthus (Eustoma grandiflorum Grise.)

Cited by 56 publications

References 22 publications

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Effects of Sequence and Expression of Eight Anthocyanin Biosynthesis Genes on Floral Coloration in Four <i>Dendrobium</i> Hybrids

Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>

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Flavonoid biosynthesis in flower petals of five lines of lisianthus (Eustoma grandiflorum Grise.)

Cited by 56 publications

References 22 publications

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Effects of Sequence and Expression of Eight Anthocyanin Biosynthesis Genes on Floral Coloration in Four <i>Dendrobium</i> Hybrids

Cloning of the Flavonoid 3&apos;-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3&apos;H</i>) and Complementation of an F3&apos;H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3&apos;H</i>

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Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>