Effects of Sequence and Expression of Eight Anthocyanin Biosynthesis Genes on Floral Coloration in Four &lt;i&gt;Dendrobium&lt;/i&gt; Hybrids

Kriangphan, Nattaphan; Vuttipongchaikij, Supachai; Kittiwongwattana, Chokchai; Suttangkakul, Anongpat; Pinmanee, Phitsanu; Sakulsathaporn, Arpakorn; Suwimon, Rawat; Suputtitada, Saowanee; Chanvivattana, Yindee; Apisitwanich, Somsak

doi:10.2503/hortj.mi-020

Cited by 18 publications

(12 citation statements)

References 42 publications

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“…It implied that the expression of Unigene0031269, Unigene0022913, Unigene0032398, Unigene0036679, Unigene0026448, and Unigene0047139 have a positive role in chlorophyll accumulation. According to the public database, Unigene0031269, Unigene0022913, Unigene0032398, Unigene0036679, Unigene0026448, and Unigene0047139 were annotated as Mg-protoporphyrinogen IX monomethylester cyclase gene ( CRD1 ), divinyl reductase gene ( DVR ), protochlorophyllide oxidoreductase gene ( POR ) [ 22 ], chlorophyllide a oxygenase gene ( CAO ), protoheme ferro-lyase gene ( HEMH ), and pheophorbide a oxygenase gene ( PAO ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Leaf in Tree Peony Reveals Differentially Expressed Pigments Genes

et al. 2017

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Tree peony (Paeonia suffruticosa Andrews) is an important traditional flower in China. Besides its beautiful flower, the leaf of tree peony has also good ornamental value owing to its leaf color change in spring. So far, the molecular mechanism of leaf color change in tree peony is unclear. In this study, the pigment level and transcriptome of three different color stages of tree peony leaf were analyzed. The purplish red leaf was rich in anthocyanin, while yellowish green leaf was rich in chlorophyll and carotenoid. Transcriptome analysis revealed that 4302 differentially expressed genes (DEGs) were upregulated, and 4225 were downregulated in the purplish red leaf vs. yellowish green leaf. Among these DEGs, eight genes were predicted to participate in anthocyanin biosynthesis, eight genes were predicted involved in porphyrin and chlorophyll metabolism, and 10 genes were predicted to participate in carotenoid metabolism. In addition, 27 MYBs, 20 bHLHs, 36 WD40 genes were also identified from DEGs. Anthocyanidin synthase (ANS) is the key gene that controls the anthocyanin level in tree peony leaf. Protochlorophyllide oxido-reductase (POR) is the key gene which regulated the chlorophyll content in tree peony leaf.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Leaf in Tree Peony Reveals Differentially Expressed Pigments Genes

et al. 2017

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“…Additionally, the expression of F3′H gradually increased during the opening of Dendrobium ‘Sonia Earsakul’ flowers, and its silencing could block the formation of endogenous anthocyanins [ 46 ]. Similarly, a low expression of F3′H resulted in white petals in Dendrobium hybrids [ 47 ]. F3′5′H is also a key gene for flavonoid biosynthesis in Dendrobium .…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Flavonoid Biosynthesis Mechanisms of Dendrobium Species by Analyzing the Transcriptome and Metabolome

Liu

Zhang

Yuan

2022

IJMS

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Dendrobium huoshanense, Dendrobium officinale, and Dendrobium moniliforme, as precious Chinese medicinal materials, have a variety of medicinal properties. Flavonoids are important medicinal components of Dendrobium, but their accumulation rules and biosynthesis mechanisms remain unclear. To explore the similarities and differences of flavonoid accumulation and biosynthesis in these three Dendrobium species, we performed flavonoid content determination, widely-targeted metabolomics and transcriptome sequencing on 1–4 years old Dendrobium species. The results showed that in different growth years, D. huoshanense stems had the highest flavonoid content in the second year of growth, while D. officinale and D. moniliforme stems had the highest flavonoid content in the third year of growth. A total of 644 differentially accumulated metabolites (DAMs) and 10,426 differentially expressed genes (DEGs) were identified by metabolomic and transcriptomic analysis. It was found that DAMs and DEGs were not only enriched in the general pathway of “flavonoid biosynthesis”, but also in multiple sub-pathways such as “Flavone biosynthesis”, and “Flavonol biosynthesis” and “Isoflavonoid biosynthesis”. According to a combined transcriptome and metabolome analysis, the expression levels of the F3'H gene (LOC110096779) and two F3'5'H genes (LOC110101765 and LOC110103762) may be the main genes responsible for the differences in flavonoid accumulation. As a result of this study, we have not only determined the optimal harvesting period for three Dendrobium plants, but also identified the key genes involved in flavonoid biosynthesis and provided a basis for further study of the molecular mechanism of flavonoid synthesis.

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“…Anthocyanin biosynthetic pathways, key enzymes as well transcription factors that affect anthocyanin content have been intensively elucidated in plants [ 13 ]. In the Orchidaceae, both chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR) have been cloned from Bromheadia finlaysoniana [ 14 ], CHS, DFR and a flavonoid 3′5′-hydroxylase (F3′5′H) from Dendrobium hybrids including ‘Sonia Earsakul’, ‘Sirin classic’, ‘Suree white’ and ‘Jasmine white’ [ 15 ] and CHS, DFR and F3′5′H from Phalaenopsis [ 16 , 17 ]. Anthocyanin synthase (ANS, EC 1.14.11.19) is responsible for converting colorless leucoanthocyanidins to colored anthocyanidins, in particular, via a conserved and well-characterized pathway in a wide range of plant species, including Antirrhinum majus , Arabidopsis thaliana , Malus sieversii , Petunia hybrida , Punica granatum , Vitis vinifera and Zea mays [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Accumulation of Anthocyanins in Dendrobium officinale Stems with Red and Green Peels

Liao

Silva³

et al. 2018

IJMS

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Dendrobium officinale stems, including red and green stems, are widely used as a dietary supplement to develop nutraceutical beverages and food products. However, there is no detailed information on pigment composition of red and green stems. Here, we investigated the content and composition of pigments in red and green stems by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry and assessed the differential accumulation of anthocyanins at the molecular level. The color of peels in red stems was caused by the presence of anthocyanins in epidermal cells unlike the peels of green stems. The glucoside derivatives delphinidin and cyanidin are responsible for the red color. Within the D. officinale anthocyanidin biosynthetic pathway, DoANS and DoUFGT, coding for anthocyanidin synthase and UDP-glucose flavonoid-3-O-glucosyltransferase, respectively, are critical regulatory genes related to the differential accumulation of anthocyanidin. These findings provide a more complete profile of pigments, especially anthocyanin, in D. officinale stems, and lay a foundation for producing functional foods.

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Effects of Sequence and Expression of Eight Anthocyanin Biosynthesis Genes on Floral Coloration in Four <i>Dendrobium</i> Hybrids

Cited by 18 publications

References 42 publications

Transcriptomic Analysis of Leaf in Tree Peony Reveals Differentially Expressed Pigments Genes

Transcriptomic Analysis of Leaf in Tree Peony Reveals Differentially Expressed Pigments Genes

A Comparison of the Flavonoid Biosynthesis Mechanisms of Dendrobium Species by Analyzing the Transcriptome and Metabolome

Differential Accumulation of Anthocyanins in Dendrobium officinale Stems with Red and Green Peels

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