Flavone production in transformed root cultures of Scutellaria baicalensis Georgi

Nishikawa, Kaneyasu; Furukawa, Hiroko; Fujioka, Toshihiro; Fujii, Hiroko; Mihashi, Kunihide; Shimomura, Koichiro; Ishimaru, Kanji

doi:10.1016/s0031-9422(99)00306-4

Cited by 46 publications

(30 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The roots produced by subsequent transfer of T-DNA have been recognized as potential alternative sources of many secondary compounds in medicinally important plants as the genetic transformation does not affect the natural root synthetic capacities (Flores and Medina-Bolivar 1995). The potential of hairy root of Scutellaria baicalensis have been investigated several time with respect to production of root specific flavones and flavonoids (Nishikawa et al 1999, Zhou et al 1997, Kuzovkina et al 2001 but no data available on morphology and growth characteristics of hairy roots, time course of accumulation of biologically active compounds (baicalin and wogonin) and establishment of hairy root liquid culture. The aim of the present investigation was the transformation of Scutellaria baicalensis by four different Agrobacterium rhizogenes strains, establishment of hairy roots cultures, and stable integration of T L -DNA and T R -DNA and expression in the plant genome.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

Tiwari¹,

Trivedi²,

Guang³

et al. 2008

Biol. plant.

View full text Add to dashboard Cite

Using different explants of in vitro seed grown Scutellaria baicalensis Georgi plantlets, hairy roots were induced following inoculation of Agrobacterium rhizogenes strains A 4 GUS, R1000 LBA 9402 and ATCC11325. The A 4 GUS proved to be more competent than other strains and the highest transformation rates were observed in cotyledonary leaf explant (42.6 %). The transformed roots appeared after 15 -20 d of incubation on hormone free Murashige and Skoog medium. Growth of hairy roots was assessed on the basis of total root elongation, lateral root density and biomass accumulation. Maximum growth rate was recorded in root:medium ratio 1:100 (m/v). Hairy root lines were further established in Gamborg B 5 medium and the biomass increase was maximum from 15 to 30 d. PCR, Southern hybridization and RT-PCR confirmed integration and expression of left and right termini-linked Ri T-DNA fragment of the Ri plasmid from A 4 GUS into the genome of Scutellaria baicalensis hairy roots. GUS assay was also performed for further integration and expression. All the clones showed higher growth rate them non-transformed root and accumulated considerable amounts of the root-specific flavonoids. Baicalin content was 14.1 -30.0 % of dry root mass which was significantly higher then that of control field grown roots (18 %). The wogonin content varies from 0.08 to 0.18 % among the hairy root clones which was also higher than in non-transformed roots (0.07 %).

show abstract

Section: Introductionmentioning

confidence: 99%

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

Tiwari¹,

Trivedi²,

Guang³

et al. 2008

Biol. plant.

View full text Add to dashboard Cite

show abstract

“…[4 -7] The chemical structure of new compounds from Scutellaria was elucidated based mainly on NMR spectroscopy evidences. [8,9] Baicalin, a polyphenol with three OH groups ( Fig. 1), has strong antioxidative and antiradical properties and can form complexes with metal ions (for example, with Fe 2+ ).…”

Section: Introductionmentioning

confidence: 99%

Solid‐state NMR studies and DFT calculations of flavonoids: baicalein, baicalin and wogonoside

Wolniak¹,

Oszmiański²,

Wawer³

2007

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

Three flavonoids of pharmaceutical importance-baicalein, baicalin, and wogonoside-were isolated from a Chinese medicinal plant Scutellaria baicalensis Georgi and studied by 13C NMR in solution and solid state. Two-dimensional (2D) NMR spectroscopy in the liquid phase and dipolar dephasing (DD) experiments in magic-angle spinning (MAS) spectra enabled the assignment of 13C resonances. The cross-polarization (CP) time constants T(CH) and relaxation times T(H) (1rho) were obtained from the variable-contact time experiments. The principal elements of the 13C chemical shift tensor were determined in the spectra recorded under slow sample spinning (2 kHz) using phase-adjusted spinning sideband (PASS)-2D NMR technique, and were verified by density functional theory gauge-independent atomic orbital (DFT GIAO) calculations of shielding constants. Analysis of the 13C delta(ii) and comparison with shielding parameters calculated for different conformers of compounds 1-3 enabled the selection of the most reliable geometry in the solid phase. In all three compounds, an intramolecular hydrogen bond C5--OH...=C4 is formed; the existence of baicalein and baicalin with 'anticlockwise' orientation of OH groups is more probable.

show abstract

“…bicolor using modifi ed MS medium & TDZ also evaluated effects of TDZ treatments for different durations of time in MS medium (containing MS salts, B5 vitamin and 3% sucrose), to induce regeneration. Similarly, shoot and callus culture techniques have also been used to observe phenolic compound in S. orientalis, S. baicalensis, S. iyoensis, S. taurica, S. laterifl ora, S. ventenatii, S. pontica, S. taurica, S. galericulata, S. integrifolia and S. alpina (Nishikawa et al 1999, Hirotani et al 1998, Zobayed et al 2004and Joshee et al 2007.…”

Section: Abstract R E G U L a R A R T I C L Ementioning

confidence: 99%

“…Aerial parts of S. orientalis are collected (Anonymous 2015) in Turkey and their extracts are used for treatment stres and many disorders in folk medicine. Scutellaria species contain sesquiterpenoids, monoterpenoids and phenylpropane derivatives iridoid glycosides (Hernandez, 1999) and glucuronide or aglucuronide type phenolic compounds (Nishikawa et al, 1999). All species of Scutellaria are rich in scutellarin, that is known to have anti cancer properties and induce apoptosis of breast and ovarian tumor cells in vitro and is long time used in treatment of cardiovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Endemic Scutellaria orientalis L. subsp. Bicolor Using Modified MS Medium and TDZ

Özdemir

Yildirim

Kahriz

2015

Emir. J. Food Agric

View full text Add to dashboard Cite

Flavone production in transformed root cultures of Scutellaria baicalensis Georgi

Cited by 46 publications

References 8 publications

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

Solid‐state NMR studies and DFT calculations of flavonoids: baicalein, baicalin and wogonoside

Micropropagation of Endemic Scutellaria orientalis L. subsp. Bicolor Using Modified MS Medium and TDZ

Contact Info

Product

Resources

About