Micropropagation of Endemic Scutellaria orientalis L. subsp. Bicolor Using  Modified MS Medium  and  TDZ

Özdemir, Fethi Ahmet; Yildirim, Mehmet Uğur; Kahriz, Parisa Pourali

doi:10.9755/ejfa.2015-04-148

Cited by 3 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the statement of Guo et al [ 14 ] that TDZ induced shoot regeneration in many plant species of the genus Scutellaria , ( Table 1 ), such an effect was not recorded. Similarly, in a study of Ozdemir et al [ 15 ], TDZ effectively stimulated the formation of callus tissue on various types of explants derived from seedlings of this species. However, on some of them, although shoot buds were clearly visible on the explants, they did not show any shoot regeneration.…”

Section: Resultsmentioning

confidence: 65%

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Gawroński

Dyduch‐Siemińska

2022

Genes

View full text Add to dashboard Cite

The in vitro culture technique can be used for micropropagation of medicinal plants as well as for creating genotypes with an improved profile of phytochemical compounds. For this purpose, somaclonal variability may be used for the induction of genetic diversity among regenerants. The paper presents a protocol for obtaining Scutellaria baicalensis regenerants by indirect organogenesis and the assessment of their genetic variability with the use of start codon-targeted markers. The most intense process of indirect shoot organogenesis was observed on Murashige and Skoog medium supplemented with kinetin and 6-Benzylaminopurine (0.5 mg × dm−3 each)—7.4 shoot per explant on average. The callogenesis process occurred on the medium supplemented with TDZ, while the medium supplemented with GA3 allowed for direct shoot organogenesis and was used for the micropropagation of regenerants. In the analysis of plantlets obtained by indirect organogenesis, 11 ScoT markers generated a total of 130 amplicons, 45 of which were polymorphic. This analysis showed genetic diversity of regenerants in relation to the donor plant as well as within them, with mean similarity among the analyzed genotypes at the level of 0.90. This study confirms that the use of in vitro cultures allows for the possibility to generate genetic variability in Scutellaria baicalensis, which can be effectively revealed with the use of the SCoT marker.

show abstract

Section: Resultsmentioning

confidence: 65%

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Gawroński

Dyduch‐Siemińska

2022

Genes

View full text Add to dashboard Cite

show abstract

“…Gharari et al [18] pointed out the high effectiveness of BAP in combinations with various concentrations of TDZ, IBA, and NAA in the regeneration of Scutellaria araxensis, concluding that the best effect in indirect shoot organogenesis was achieved with the combination of BAP and TDZ. According to a study by Ozdemir et al [14] on S. orientalis subsp. bicolor, the callus could be regenerated in the presence of BAP alone or in combination with NAA.…”

Section: In Vitro Culture Analysismentioning

confidence: 99%

“…These techniques also contribute to the conservation of endangered and endemic medicinal plant species [13]. So far, research on the in vitro micropropagation of the Scutellaria genus has focused on species, such as S. orientalis [14,15], S. alpine [16,17], S. araxiensis [18], S. barbata, and S. racemosa [19]. They indicate the possibility of regeneration by both indirect and direct organogenesis.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

Dyduch-Siemińska,

Gawroński

2024

Applied Sciences

View full text Add to dashboard Cite

The efficiency and method of regeneration in in vitro culture conditions depend primarily on the plant growth regulators (PGRs) used. Even growth regulators belonging to one group may have different effects, stimulating the process of direct or indirect organogenesis, thus possibly disturbing the genetic stability among regenerants. The main aim of this study was to identify the genetic stability of Scutellaria baicalensis regenerates obtained by in vitro culture method using start codon targeted (ScoT) markers. S.baicalensis nodal explants were regenerated on MS medium supplemented with kinetin (KIN) at concentrations of 0.25, 0.5, 1.0, and 2.0 mg × dm−3 or benzylaminopurine (BAP)—0.25, 0.5, 1.0, and 2.0 mg × dm−3. The effects of the number of propagated shoots, length, number of nodes, and fresh mass of regenerants were assessed. Moreover, the genetic stability of the regenerants was analyzed using start codon targeted (SCoT) markers. Direct shoot organogenesis was observed on an MS medium containing kinetin, while indirect shoot induction occurred on an MS medium supplemented with BAP. The highest average number of shoots (3.6) was achieved for the MS + KIN medium at a concentration of 0.25 and 5.8 for the MS + BAP 1.0 medium. The average length and average number of nodes were the highest on the MS + BAP 0.25 medium (50.0 and 6.0, respectively), while the lowest values of these features were observed on the MS + KIN 2.0 medium (40.3 and 4.9, respectively). A total of 111 amplified bands were exhibited by SCoT primers. Three of the analyzed primers revealed four unique genotype-specific markers. The average percentage of polymorphism obtained was 36.7%. The analysis of genetic similarity revealed a high level of genetic similarity between the donor plant and regenerants obtained on MS “0” (medium without the addition of phytohormones). A slightly lower value of genetic similarity was observed for regenerants obtained by direct organogenesis (MS + KIN medium at all concentrations). Indirect shoot organogenesis observed on the MS + BAP medium (all concentrations) resulted in the highest differentiation, both in relation to the donor plant and MS “0” regenerants. The results of our work indicate that, in the case of S. baicalensis, the maintenance of genetic stability depends primarily on the presence of the cytokinin type in the medium.

show abstract

“…Türkiye'de şeker pancarı ıslahında; bitki doku kültürü çalışmalarıyla genetik materyalin klonla [19][20][21][22][23][24][25][26][27], hücre kültürüyle [28], kallus kültürüyle [15], ovül kültürüyle [21,29] ve protoplast kültürüyle [30], çoğaltılmasına yönelik bir takım çalışmalar yürütülmüştür. Bitki doku kültürü çalışmalarıyla; aynı genetik yapıya sahip birçok birey; hızlı ve sağlıklı olarak çoğaltılabilmektedir [15,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37]. Ancak, ıslah programına dâhil edilen önemli kültür bitkilerine yakın akraba doğal türlerinin bitki doku kültürü teknikleriyle çoğaltılmasına yönelik en uygun koşulların belirlenmesi ve tespiti için yeni çalışmalara ihtiyaç duyulmaktadır.…”

Section: Introductionunclassified

“…2,3,5 triiyodobenzoik asit (0,5 mg/1) ile N6-benzil-ammopurin (1 mg/L)'den oluşan hormon kombinasyonunun 21 ± 1 günlük fidelerde içsel bitkisel hormon dengesini değiştirmek için en iyi kombinasyon olduğunu belirtmişlerdir[43]. Doku kültürü çalışmalarında, gelişen sürgünlerin in-vitro ortamda köklendirilmesine yönelik, çoğunlukla oksin grubu hormonların kullanıldığı kültür ortamları kullanılmaktadır[32,33,36]. Gürel ve ark.…”

unclassified

Kıyı Pancarı (Beta vulgaris subsp. maritima (L.) Arcang) Türünün In-vitro Şartlarda Mikro Çoğaltımı

KOLÇAK>¹,

Sarihan

2022

Uşak Üniversitesi Fen Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

Bu çalışmada kıyı pancarı (Beta vulgaris subsp. maritima (L.) Arcang) bitkisinin in-vitro koşullarda farklı eksplantlarının değişik bitki büyüme düzenleyicisi (Kinetin ve Indol bütirik asit) kombinasyonlarında rejenerasyon yeteneklerinin belirlenmesi amaçlanmıştır. Kinetin (KIN) ve Indol-3-bütirik asit (İBA)’in farklı dozları (0; 0,5; 1,0; 1,5 ve 2,0 mg/L) ile hazırlanan besi ortamlarında hipokotil, boğum ve kotiledon eksplantlarının rejenerasyon yetenekleri belirlenmiştir. Çalışmada kallus oluşturma oranı (%), sürgün oluşturma oranı (%), köklenme oranı (%), eksplant başına sürgün sayısı (adet), kök sayısı (adet) ve en uzun kök uzunluğu (mm) gibi karakterlerde ölçümler yapılmıştır. Elde edilen sonuçlara göre en iyi sürgün oluşturma oranı (%100) boğum eksplantlarından elde edilmiştir. Bu eksplantın tüm uygulamalarında aynı sonuç elde edilmiştir. Bu eksplantın sürgünlerinin kök oluşum oranı kinetinsiz ortamda, 0,5, 1,5 ve 2,0 mg/L İBA dozlarının uygulandığı ortamlarda %100 olarak gerçekleşmiştir. Elde edilen sonuçlara göre boğum eksplantında 0,0 mg/L KİN + 1,5 mg/L İBA dozunun bu bitki türünün in-vitro çoğaltılmasında en etkili şekilde kullanılabilecek bitki büyüme düzenleyicileri kombinasyonu olduğu belirlenmiştir. Bu bitki türünün hızlı çoğaltılmasında boğum eksplantının çok uygun bir şekilde bitkinin hızlı çoğaltılmasında kullanılabileceği tespit edilmiştir.

show abstract

Micropropagation of Endemic Scutellaria orientalis L. subsp. Bicolor Using Modified MS Medium and TDZ

Cited by 3 publications

References 15 publications

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

Kıyı Pancarı (Beta vulgaris subsp. maritima (L.) Arcang) Türünün In-vitro Şartlarda Mikro Çoğaltımı

Contact Info

Product

Resources

About